专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

2. 发明申请

WO2007017229A3 METHOD FOR CARRYING OUT THE SELECTIVE EVOLUTION OF PROTEINS IN VITRO 审中-公开
标题翻译：过程在蛋白质的不断发展目标锁定在体外
公开(公告)号：WO2007017229A3
公开(公告)日：2007-05-18
申请号：PCT/EP2006007798
申请日：2006-08-07
申请人： GENEART AG , LISS MICHAEL
发明人： LISS MICHAEL
IPC分类号： C12N15/10 , C40B10/00
CPC分类号： C12N15/1058 , C12N15/1055 , C12N15/1075 , C40B10/00
摘要： The invention relates to the production of variants of a protein in an in vitro evolution method, comprising the steps: (A) preparing an in vitro expression system, comprising: (i) a nucleic acid sequence S, which codes for a protein Y to be varied; (ii) a target molecule X 1 capable of binding to protein Y and/or at least one variant Y thereof; (iii) an RNA polymerase (Pol) capable of transcribing the nucleic acid sequence S; (iv) a reverse transcriptase (RT) capable of reverse transcribing transcripts of nucleic acid sequence S, the target molecule X being coupled to Pol and protein Y to RT, or target molecule X being coupled to RT and protein Y being coupled to Pol; (B) incubating the in vitro expression system from (A) under conditions that enable a transcription, a reverse transcription and a translation while forming variants Y' of protein Y and nucleic acid sequences S' coding therefor, and which promote the formation of variants Y' with improved binding properties for target molecule X; (C) isolating and optionally characterizing those variants Y' that have improved binding properties for binding to X and/or isolation nucleic acid sequence variants S' that code for Y'.
摘要翻译：本发明涉及生产在体外进化方法，包括以下步骤的蛋白的变体的：（A）提供一种在体外表达系统包括（i）的核酸序列S这对于一个被改变蛋白质Y代码，（II）目标分子X _{1 其能够与蛋白质Y和/或至少一种变体y以它们的结合，（ⅲ）RNA聚合酶（POL），其能够转录的核酸序列S，（ⅳ）反转录酶（RT），其能够，S转录的核酸序列的反向，其中任目标分子X耦合到Pol和蛋白质y以RT，或目标分子X到的转录物 RT和条件转录，逆转录和翻译，以形成下孵育选自（a）在体外表达系统中的蛋白Y被耦合至波尔，（B）变体ÿ^{Protei的 NS Y和对编码核酸序列小号^{允许和变体ÿ^{的形成的青睐与目标分子X分离和任选地表征那些变体改善的结合性质，（C） ÿ^{，它具有改进的结合特性的结合X，和/或用于分离ÿ^{'的核酸序列编码变体取值^。}}}}}}

IPRDB

热门服务

关于我们

友情链接

联系方式