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    • 2. 发明申请
    • METHODS FOR FORMING PATTERNED STRUCTURES
    • 形成图案化结构的方法
    • WO2008022097A3
    • 2008-07-03
    • PCT/US2007075856
    • 2007-08-14
    • API NANOFABRICATION AND RES CODENG XUEDONGWANG JIAN JIMCHEN LEINIKOLOV ANGUEL NO'BRIEN NADALIU XIAOMING
    • DENG XUEDONGWANG JIAN JIMCHEN LEINIKOLOV ANGUEL NO'BRIEN NADALIU XIAOMING
    • H01L21/3205H01L21/4763
    • G02B5/1857
    • In general, in a first aspect, the invention features a method that includes forming a first layer comprising a first material over a surface of a second layer, wherein forming the first layer includes sequentially forming a plurality of monolayers of the second material over the surface of the second layer, the second layer comprises a plurality of rows of a second material extending along a first direction and spaced from one another in a second direction orthogonal to the first direction, and the first layer conforms to the surface of the second layer. The method further includes removing portions of the first layer to produce a plurality of rows of the first material extending along the first direction and spaced from one another in the second direction and removing portions of a third layer comprising a third material, where the portions correspond to intervals between the second plurality of rows so that removing the portions forms a plurality of rows of the third material extending along the first direction and spaced apart from one another. The first and second materials are different.
    • 通常,在第一方面,本发明的特征在于一种方法,该方法包括在第二层的表面上方形成包含第一材料的第一层,其中形成第一层包括在表面上顺序地形成多个单层的第二材料 第二层包括沿第一方向延伸且在与第一方向正交的第二方向上彼此间隔开的多行第二材料,并且第一层符合第二层的表面。 该方法进一步包括去除第一层的多个部分以产生沿着第一方向延伸且在第二方向上彼此间隔开的多行第一材料并且去除包括第三材料的第三层的部分,其中这些部分对应于 到第二多个行之间的间隔,使得去除这些部分形成沿着第一方向延伸并且彼此间隔开的多行第三材料。 第一种和第二种材料是不同的。
    • 4. 发明申请
    • TRANSGENIC ANIMAL MODEL FOR SPLICEOSOME-MEDIATED RNA TRANS-SPLICING
    • 用于SPLICEOSOME介导RNA转移分离的转基因动物模型
    • WO03093414A2
    • 2003-11-13
    • PCT/US0313161
    • 2003-04-30
    • INTRONN INCPUTTARAJU MADAIAHMITCHEL LLOYDENGELHARDT JOHN FBLANCO MARIANO GARCIALIU XIAOMING
    • PUTTARAJU MADAIAHMITCHEL LLOYDENGELHARDT JOHN FBLANCO MARIANO GARCIALIU XIAOMING
    • A01K67/027C12N15/09C12N15/85C12Q1/68C12N
    • C12N15/8509A01K67/0275A01K2217/05A01K2227/105A01K2267/0393C12N2800/30
    • The present invention relates to development of an animal model system for in vivo testing of spliceosome-mediated RNA trans-splicing reactions. The present invention provides transgenic animals, and methods for generating such animals, that have been genetically engineered to expresses a target precursor messenger RNA molecule (target pre-mRNA) that serves as a substrate for a trans-splicing reaction. Specifically, the transgenic animals contain at least one transgene capable of expressing a target pre-mRNA molecule. The invention provides methods, based on utilization of the transgenic animals, for assessing the specificity and efficiency of a pre-trans-splicing molecule (PTM) designed to interact with a target pre-mRNA and mediate a trans-splicing reaction resulting in the generation of a novel chimeric RNA molecule. The present invention further relates to the transgenic expression of PTM molecules in animals to determine gene function, i.e, functional genetics. The present invention is based on the successful generation of a transgenic animal expressing a target pre-mRNA and, moreover, the use of that animal to detect accurate in vivo trans-splicing reactions in the presence of a PTM.
    • 本发明涉及用于体内测试剪接体介导的RNA反式剪接反应的动物模型系统的开发。 本发明提供了转基因动物,以及用于产生这样的动物的方法,其被遗传工程化以表达用作转拼反应的底物的靶前体信使RNA分子(靶前体mRNA)。 具体地,转基因动物含有能够表达靶mRNA前分子的至少一个转基因。 本发明提供了基于转基因动物利用的方法,用于评估设计成与靶前体mRNA相互作用的转录前分子(PTM)的特异性和效率,并介导导致产生转录的反式剪接反应 的新型嵌合RNA分子。 本发明还涉及PTM分子在动物中的转基因表达以确定基因功能,即功能遗传学。 本发明基于成功生成表达靶mRNA的转基因动物,此外,该技术在PTM存在下使用该动物检测精确的体内转拼反应。