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1. 发明申请

WO0116810A3 A COMPUTER-BASED METHOD FOR MACROMOLECULAR ENGINEERING AND DESIGN 审中-公开
标题翻译：一种用于大分子工程和设计的基于计算机的方法
公开(公告)号：WO0116810A3
公开(公告)日：2002-05-02
申请号：PCT/EP0008504
申请日：2000-08-31
申请人： EUROPEAN MOLECULAR BIOLOGY LAB EMBL , LACROIX EMMANUEL , SERRANO LUIS
发明人： LACROIX EMMANUEL , SERRANO LUIS
IPC分类号： C07K1/00 , G06F17/50
CPC分类号： C07K1/00 , G06F19/16
摘要： The present invention relates to a system and method for engineering and designing a macromolecule. An experimentally determined or de novo atomic structure that corresponds to the macromolecule is identified. The atomic structure is composed of building blocks. When the macromolecule is a peptide or a protein, the building blocks are amino acid residues. A target subset of the building blocks in the atomic structure to be optimized is identified. The coordinates of those building blocks that are not in the target subset are fixed. For each building block in the target subset, a large number of potential conformers is sampled. Each conformer to be sampled is substituted into the atomic structure and tested against an energy function that includes the equivalent energy of the conformer in a reference state and comprises at least one entropic term. Combinations of conformers that best satisfy an interaction energy function are identified.
摘要翻译：本发明涉及一种用于工程设计大分子的系统和方法。确定对应于大分子的实验确定的或从未确定的原子结构。原子结构由构建块组成。当大分子是肽或蛋白质时，构建单元是氨基酸残基。识别要优化的原子结构中的构建块的目标子集。不在目标子集中的构件块的坐标是固定的。对于目标子集中的每个构建块，对大量潜在构象进行采样。要采样的每个构象异构体被替换为原子结构，并针对包括构象异构体在参考状态中的等效能量的能量函数进行测试，并且包括至少一个熵项。确定最能满足相互作用能量函数的构象异构体的组合。

2. 发明申请

WO2004067736A3 CUSTOM-MADE MEGANUCLEASE AND USE THEREOF 审中-公开
标题翻译：自定义MEGANUCLEASE及其使用
公开(公告)号：WO2004067736A3
公开(公告)日：2004-11-25
申请号：PCT/IB2004000827
申请日：2004-01-28
申请人： CELLECTIS , ARNOULD SYLVAIN , BRUNEAU SYLVIA , CABANIOLS JEAN-PIERRE , CHAMES PATRICK , CHOULIKA ANDRE , DUCHATEAU PHILIPPE , EPINAT JEAN-CHARLES , GOUBLE AGNES , LACROIX EMMANUEL , PAQUES FREDERIC , PEREZ-MICHAUT CHRISTOPHE , SMITH JULIANNE , SOURDIVE DAVID
发明人： ARNOULD SYLVAIN , BRUNEAU SYLVIA , CABANIOLS JEAN-PIERRE , CHAMES PATRICK , CHOULIKA ANDRE , DUCHATEAU PHILIPPE , EPINAT JEAN-CHARLES , GOUBLE AGNES , LACROIX EMMANUEL , PAQUES FREDERIC , PEREZ-MICHAUT CHRISTOPHE , SMITH JULIANNE , SOURDIVE DAVID
IPC分类号： A01K67/027 , A61K38/00 , A61K38/46 , A61K48/00 , C12N9/22 , C12N15/55 , C12N15/85 , C12N15/90 , A01H1/00 , C12N15/10
CPC分类号： C12N15/907 , A01K67/0275 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/105 , A01K2267/03 , A01K2267/0337 , A61K38/00 , A61K38/1709 , A61K38/465 , A61K48/00 , A61K48/0058 , C07K14/435 , C07K2319/81 , C12N7/00 , C12N9/22 , C12N15/1058 , C12N15/8509 , C12N15/86 , C12N15/90 , C12N2730/10143 , C12N2799/022 , C12N2800/80 , C12N2830/002 , C12N2830/55 , C12N2840/20 , C12N2840/44 , C12Y301/00 , C12Y301/21004
摘要： New rare-cutting endonucleases, also called custom-made meganucleases, which recognize and cleave a specific nucleotide sequence, derived polynucleotide sequences, recombinant vector cell, animal, or plant comprising said polynucleotide sequences, process for producing said rare-cutting endonucleases and any use thereof, more particularly, for genetic engineering, antiviral therapy and gene therapy.
摘要翻译：新的稀释内切核酸酶，也称为定制的大范围核酸酶，其识别和切割特定的核苷酸序列，衍生的多核苷酸序列，包含所述多核苷酸序列的重组载体细胞，动物或植物，用于产生所述稀少内切核酸酶的方法和任何用途更具体地，涉及基因工程，抗病毒治疗和基因治疗。

3. 发明申请

WO2004067753A3 USE OF MEGANUCLEASES FOR INDUCING HOMOLOGOUS RECOMBINATION EX VIVO AND IN TOTO IN VERTEBRATE SOMATIC TISSUES AND APPLICATION THEREOF. 审中-公开
标题翻译：使用大分子诱导体外同源重组和在脊椎动物体细胞组织中的多巴胺及其应用。
公开(公告)号：WO2004067753A3
公开(公告)日：2004-09-16
申请号：PCT/IB2004000848
申请日：2004-01-28
申请人： CELLECTIS , ARNOULD SYLVAIN , BRUNEAU SYLVIA , CABANIOLS JEAN-PIERRE , CHAMES PATRICK , CHOULIKA ANDRE , DUCHATEAU PHILIPPE , EPINAT JEAN-CHARLES , GOUBLE AGNES , LACROIX EMMANUEL , PAQUES FREDERIC , PEREZ-MICHAUT CHRISTOPHE , SMITH JULIANNE , SOURDIVE DAVID
发明人： ARNOULD SYLVAIN , BRUNEAU SYLVIA , CABANIOLS JEAN-PIERRE , CHAMES PATRICK , CHOULIKA ANDRE , DUCHATEAU PHILIPPE , EPINAT JEAN-CHARLES , GOUBLE AGNES , LACROIX EMMANUEL , PAQUES FREDERIC , PEREZ-MICHAUT CHRISTOPHE , SMITH JULIANNE , SOURDIVE DAVID
IPC分类号： A01K67/027 , A61K38/00 , A61K38/46 , A61K48/00 , C12N9/22 , C12N15/55 , C12N15/85 , C12N15/90
CPC分类号： C12N15/907 , A01K67/0275 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/105 , A01K2267/03 , A01K2267/0337 , A61K38/00 , A61K38/1709 , A61K38/465 , A61K48/00 , A61K48/0058 , C07K14/435 , C07K2319/81 , C12N7/00 , C12N9/22 , C12N15/1058 , C12N15/8509 , C12N15/86 , C12N15/90 , C12N2730/10143 , C12N2799/022 , C12N2800/80 , C12N2830/002 , C12N2830/55 , C12N2840/20 , C12N2840/44 , C12Y301/00 , C12Y301/21004
摘要： Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and to its application for genome engineering and gene therapy.
摘要翻译：使用大范围核酸酶在体外和体内诱导同源重组以及在脊椎动物体细胞组织中的同源重组以及其在基因组工程和基因治疗中的应用。

4. 发明申请

WO2004015134A3 METHOD OF DETERMINING THE CLEAVAGE ACTIVITY OF AN ENDONUCLEASE 审中-公开
标题翻译：测定内切核酸酶切割活性的方法
公开(公告)号：WO2004015134A3
公开(公告)日：2004-04-15
申请号：PCT/FR0302476
申请日：2003-08-06
申请人： CELLECTIS , ARNOULD SYLVAIN , CHAMES PATRICK , CHOULIKA ANDRE , LACROIX EMMANUEL
发明人： ARNOULD SYLVAIN , CHAMES PATRICK , CHOULIKA ANDRE , LACROIX EMMANUEL
IPC分类号： C12Q1/44 , C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6809 , C12Q1/44 , C12Q1/6823 , G01N2333/922 , C12Q2521/319 , C12Q2521/301
摘要： The invention relates to a method of testing the cleavage activity of an endonuclease in relation to a DNA substrate. The inventive method is based on the discrimination of the cleaved DNA substrate and the intact substrate. The aforementioned discrimination can be performed by means of selective degradation or depletion of the intact substrate or by selective amplification of the cleaved substrate. Said method can be applied to the screening and the selection of an endonuclease or a cleavage site for an endonuclease.
摘要翻译：本发明涉及测试内切核酸酶相对于DNA底物的切割活性的方法。本发明的方法基于对切割的DNA底物和完整底物的区分。上述鉴别可以通过完整底物的选择性降解或消耗或通过选择性扩增解离的底物来进行。所述方法可以应用于内切核酸酶或内切核酸酶切割位点的筛选和选择。

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