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1. 发明申请

WO2009072715A1 MICRO-SCALED ANIMAL CELL INCUBATOR AND PRODUCTION METHOD THEREOF 审中-公开
标题翻译：微量级动物细胞培养物及其生产方法
公开(公告)号：WO2009072715A1
公开(公告)日：2009-06-11
申请号：PCT/KR2008/003741
申请日：2008-06-27
申请人： ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTE , CHOI, Yo Han , CHUNG, Kwang Hyo , LEE, Dae Sik , KIM, Sang Hee , JEONG, Min Suk , JUN, Chi Hoon , JUNG, Moon Youn , PARK, Seon Hee
发明人： CHOI, Yo Han , CHUNG, Kwang Hyo , LEE, Dae Sik , KIM, Sang Hee , JEONG, Min Suk , JUN, Chi Hoon , JUNG, Moon Youn , PARK, Seon Hee
IPC分类号： C12M3/00
CPC分类号： C12M23/20 , B01L3/5027 , B01L7/00 , C12M23/16 , C12M41/12 , Y10T29/49124
摘要： There is provided a cell culture technology for culturing an animal cell in a separate microstructure. The micro-scaled animal cell incubator includes a lower glass substrate having fine hot wires processed with a metal and formed in an upper surface thereof; a first PDMS film attached onto the lower glass substrate to form two or more liquid and solid storage spaces in a position corresponding to the fine hot wires of the lower glass substrate and gas flow channels coupled respectively to the liquid and solid storage spaces to allow generated gases to flow therethrough; a gas-permeable PDMS thin film attached onto the gas flow channels of the PDMS film to pass the generated gases therethrough; a second PDMS film attached onto the PDMS thin film and having a culture medium storage space for storing a culture medium; and an upper glass substrate attached onto the second PDMS film and having fine hot wires formed in a lower surface thereof, the fine hot wires being covered by the PDMS film. The micro-scaled animal cell incubator may be useful to control a temperature of a culture medium to a suitable temperature level, as well as to self-supply gases required for the animal cell culture in a microstructure.
摘要翻译：提供了用于在单独的微结构中培养动物细胞的细胞培养技术。微型动物细胞培养箱包括具有用金属加工并形成在其上表面的细微热丝的下玻璃基板; 附着在下玻璃基板上的第一PDMS膜，以形成两个或更多个液体和固体存储空间，该液体和固体储存空间位于对应于分别与液体和固体储存空间相连的下部玻璃基板和气体流动通道的细微热线的位置，以允许产生气体流过其中; 透气的PDMS薄膜，附着在PDMS薄膜的气流通道上，以使产生的气体通过; 附着在PDMS薄膜上并具有用于储存培养基的培养基存储空间的第二PDMS膜; 以及附着在第二PDMS膜上并具有形成在其下表面上的精细热丝的上玻璃基板，细的热丝被PDMS膜覆盖。微量级动物细胞培养箱可用于将培养基的温度控制到合适的温度水平，以及自动供应在微结构中动物细胞培养所需的气体。

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