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    • 2. 发明申请
    • MONOCLONAL ANTIBODY RECOGNIZING SERUM AMYLOID A
    • 单克隆抗体识别血清AMYLOID A
    • WO1997008335A1
    • 1997-03-06
    • PCT/JP1996002342
    • 1996-08-22
    • EIKEN KAGAKU KABUSHIKI KAISHAHIRANO, NorihitoYAMADA, Michiko
    • EIKEN KAGAKU KABUSHIKI KAISHA
    • C12P21/08
    • C07K16/18G01N33/6896
    • A monoclonal antibody which enables immunological assay of human serum amyloid A (SAA) which serves as a marker for inflammation, etc. on the basis of the agglutination reaction thereof; an immunological assay reagent containing the monoclonal antibody; and an immunological assay method with the use of the monoclonal antibody. The immunological assay method with the use of the agglutination reaction of SAA has an improved specificity and the reagent has stabilized qualities. Thus, it has become possible to immunologically assay SAA based on its agglutination reaction by using the monoclonal antibody, different from the conventional methods with the use of polyclonal antibodies which can scarcely give a high specificity and the uniform qualities of which can be hardly maintained.
    • 基于其凝集反应,能够进行作为炎症的标记物的人血清淀粉样蛋白A(SAA)的免疫学测定的单克隆抗体; 含有单克隆抗体的免疫测定试剂; 以及使用单克隆抗体的免疫测定方法。 使用SAA的凝集反应的免疫学测定方法具有改进的特异性,并且试剂具有稳定的特性。 因此,通过使用与常规方法不同的单克隆抗体,基于其凝集反应可以免疫测定SAA,使用几乎不能提供高特异性的多克隆抗体,并且几乎不能保持其均一的质量。
    • 4. 发明申请
    • ANTIBODY THAT RECOGNIZES SERUM AMYLOID A
    • 识别血清AMYLOID A的抗体
    • WO1997006184A1
    • 1997-02-20
    • PCT/JP1996002219
    • 1996-08-07
    • EIKEN KAGAKU KABUSHIKI KAISHAEITOKU, HiromiMAEKAWA, HiroakiNEMOTO, JiroWADA, Atsufumi
    • EIKEN KAGAKU KABUSHIKI KAISHA
    • C07K16/18
    • C07K16/18G01N33/68
    • An antibody useful in the immunoassay on the basis of the agglutination of human serum amyloid A (SAA) which serves as a marker of inflammation, etc., and the use of this antibody. The antibody is a novel one which recognizes SAA and also has the activity of binding to SAA contained in the gel filtration fractions of molecular weights of 10 to 40 kD. Also, a reagent and method for the immunoassay of SAA and a method for purifying SAA by using this antibody are provided. This novel antibody having an excellent binding activity enables the assay of SAA based on the immunological agglutination thereof. This antibody is excellent in the titer against SAA and contributes not only to the specific assay of SAA at a high sensitivity but also to the widening of the assay scope.
    • 基于作为炎症等的标记物的人血清淀粉样蛋白A(SAA)的凝集,以及使用该抗体,可用于免疫测定的抗体。 该抗体是识别SAA的新型抗体,并且还具有与分子量为10〜40kD的凝胶过滤馏分中所含的与SAA结合的活性。 另外,提供了SAA的免疫测定试剂和方法以及使用该抗体来纯化SAA的方法。 具有优异结合活性的这种新型抗体能够基于其免疫凝集来测定SAA。 该抗体对SAA的滴度优异,并且不仅对SAA的特异性测定具有高灵敏度,而且还有助于测定范围的扩大。