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    • 2. 发明申请
    • ASSAY
    • WO2013005003A1
    • 2013-01-10
    • PCT/GB2012/051458
    • 2012-06-22
    • DUPONT NUTRITION BIOSCIENCES APSISAKSEN, Mai FaurschouKELLETT-SMITH, Anja HemmingsenMCLENNAN, NeilGILBERT, CeinwenKYNEB, Majbritt Hauge
    • ISAKSEN, Mai FaurschouKELLETT-SMITH, Anja HemmingsenMCLENNAN, NeilGILBERT, CeinwenKYNEB, Majbritt Hauge
    • G01N33/558G01N33/573C12Q1/42
    • G01N33/573C12Q1/00C12Q1/34C12Q1/42G01N33/558G01N2333/914G01N2333/916G01N2333/924
    • The present invention discloses an assay device (1) for detecting active enzyme in a sample. Said assay device (1) comprises the following components:- (a) a placement region (10) onto which the sample can be placed; (b) a matrix (20) operably connected to said placement region (10) such that the sample when present (such as placed) on said placement region (10) can migrate along said matrix (20); (c) at least one distinct capture location (30) on said matrix (20), wherein each distinct capture location (30) is distanced away from the placement region (10), and wherein the sample can migrate across said distinct capture location (30); (d) capture means (40) being present at or defining each distinct capture location (30), wherein said capture means (40) are capable of binding to said enzyme such that at least a portion of said sample of said enzyme is retained at at least one distinct capture location (30); and (e) selective indication means (50), or at least a component thereof, to provide selective indication of the presence of active enzyme bound to said capture means (40).
    • 本发明公开了一种用于检测样品中活性酶的测定装置(1)。 所述测定装置(1)包括以下组件: - (a)放置区域(10),所述样品可置于其上; (b)可操作地连接到所述放置区域(10)的矩阵(20),使得当所述放置区域(10)上存在的样品(例如放置)可以沿着所述基质(20)迁移; (c)所述矩阵(20)上的至少一个不同的捕获位置(30),其中每个不同的捕获位置(30)远离所述放置区域(10)远离,并且其中所述样品可以迁移穿过所述不同的捕获位置 30); (d)存在于或限定每个不同捕获位置(30)的捕获装置(40),其中所述捕获装置(40)能够结合所述酶,使得所述酶的所述样品的至少一部分保留在 至少一个不同的捕获位置(30); (e)选择性指示装置(50)或其至少一个组分,以提供与所述捕获装置(40)结合的活性酶的存在的选择性指示。