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    • 7. 发明申请
    • PROTEASES WITH MODIFIED PROPEPTIDE REGIONS
    • 具有改性的优势地区的保护
    • WO2016205710A1
    • 2016-12-22
    • PCT/US2016/038177
    • 2016-06-17
    • DANISCO US INC.
    • BABE, Lilia MariaFIORESI, Carol MarieGOEDEGEBUUR, FritsMULDER, Harm
    • C12N9/54C12N15/67
    • C12N9/54C07K2319/02C07K2319/50C12Y304/21062
    • The present invention provides methods and compositions for the production of mature proteases in bacterial host cells. The compositions include polynucleotides encoding serine protease sequences with modified or heterologous propeptide regions; polypeptides comprising serine proteases with modified or heterologous propeptide regions; expression cassettes, DNA constructs, vectors, and chromosomes comprising such polynucleotides; and bacterial host cells comprising such polynucleotides. The methods include methods for enhancing the production of mature proteases in bacterial host cells (e.g. Bacillus sp. host cells). The produced proteases find use in the industrial production of enzymes, suitable for use in various industries, including but not limited to the cleaning, animal feed and textile processing industry.
    • 本发明提供了用于在细菌宿主细胞中生产成熟蛋白酶的方法和组合物。 组合物包括编码具有修饰或异源前肽区域的丝氨酸蛋白酶序列的多核苷酸; 包含具有修饰或异源前肽区域的丝氨酸蛋白酶的多肽; 表达盒,DNA构建体,载体和包含这种多核苷酸的染色体; 和包含这种多核苷酸的细菌宿主细胞。 所述方法包括用于增强细菌宿主细胞(例如芽孢杆菌宿主细胞)中成熟蛋白酶的产生的方法。 产生的蛋白酶可用于工业生产酶,适用于各种行业,包括但不限于清洁,动物饲料和纺织加工业。