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1. 发明申请

WO0112833A3 PROCESS FOR THE BIOLOGICAL PRODUCTION OF 1,3-PROPANEDIOL 审中-公开
标题翻译： 1,3-丙二醇生物生产工艺
公开(公告)号：WO0112833A3
公开(公告)日：2001-08-30
申请号：PCT/US0022874
申请日：2000-08-18
申请人： DU PONT , GENENCOR INT , EMPTAGE MARK , HAYNIE SHARON , LAFFEND LISA , PUCCI JEFF , WHITED GREG
发明人： EMPTAGE MARK , HAYNIE SHARON , LAFFEND LISA , PUCCI JEFF , WHITED GREG
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/04 , C12N15/52 , C12P7/18 , C12R1/01 , C12R1/19 , C12N15/70
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3 , and orfZ , all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae . In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase ( dhaT ). The dramatically improved process relies on the presence in E. Coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
摘要翻译：本发明提供了从单一微生物中的可发酵碳源生物制备1,3-丙二醇的改进方法。在本发明的一个方面，通过使用E，实现了将葡萄糖转化成1,3-丙二醇的改进方法。大肠杆菌用肺炎克雷伯杆菌dha调节基因dhaR或orfY，dhaT，orfX，orfW，dhaB1，dhaB2，dhaB3，和/ 所有这些基因排列在与野生型肺炎克雷伯杆菌中相同的遗传组织中。在本发明的另一方面，使用重组体E从葡萄糖生产1,3-丙二醇的改进方法。含有编码G3PDH，G3P磷酸酶，脱水酶和脱水酶再活化因子的基因组，与使用重组E的相同方法相比。含有编码G3PDH，G3P磷酸酶，脱水酶，脱水酶活化因子和1,3-丙二醇氧化还原酶（dhaT）的基因的大肠杆菌。显着改进的过程依赖于在E中的存在。大肠杆菌编码非常特异的催化活性的基因，足以将3-羟基丙醛转化成1,3-丙二醇。

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