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1. 发明申请

WO2007062936A3 DNA BINDING SITE OF A TRANSCRIPTIONAL ACTIVATOR USEFUL IN GENE EXPRESSION 审中-公开
标题翻译：用于基因表达的转录激活因子的DNA结合位点
公开(公告)号：WO2007062936A3
公开(公告)日：2007-07-26
申请号：PCT/EP2006067734
申请日：2006-10-24
申请人： DSM IP ASSETS BV , PARENICOVA LUCIE , PEIJ VAN NOEL NICOLAAS MARIA E
发明人： PARENICOVA LUCIE , PEIJ VAN NOEL NICOLAAS MARIA E
IPC分类号： C12N15/80
CPC分类号： C12N15/80
摘要： We have discovered DNA binding sites which are specifically recognized by PrtT, a transcriptional activator for protease genes. The DNA binding site can be defined structurally by a consensus nucleotide sequence and functionally by PrtT's ability to regulate transcriptional activation through that sequence. Both PrtT and its cognate DNA binding site (i.e., the nucleotide sequence in each promoter that is recognized by PrtT) can be used in a gene expression system. Possession of only a PrtT transcriptional activator is insufficient, its cognate DNA binding site is necessary for recognition by PrtT (i.e., binding to the site and activating transcription under appropriate conditions). A functional site, such as one obtained from a wild-type fungal gene, will confer PrtT- dependent transcriptional activation on 3'-downstream sequences. A mutation of a wild- type promoter that results in a non-functional site will abolish PrtT-dependent transcriptional activation of 3'-downstream sequences. A mutation of a wild-type promoter that results in a more functional site will enhance PrtT-dependent transcriptional activation of 3'-downstream sequences.
摘要翻译：我们已经发现了由蛋白酶基因的转录激活物PrtT特异性识别的DNA结合位点。 DNA结合位点可以通过共有核苷酸序列在结构上定义，并且功能上由PrtT通过该序列调节转录激活的能力。 PrtT及其同源DNA结合位点（即由PrtT识别的每个启动子中的核苷酸序列）均可用于基因表达系统。只有PrtT转录激活物的拥有不足，其同源DNA结合位点对于PrtT识别（即在适当条件下结合位点并激活转录）是必需的。诸如从野生型真菌基因获得的功能位点将赋予3'-下游序列上的PrtT依赖性转录激活。导致非功能位点的野生型启动子的突变将消除3'-下游序列的PrtT依赖性转录激活。导致更多功能位点的野生型启动子的突变将增强3'-下游序列的PrtT依赖性转录激活。

2. 发明申请

WO2006040312A2 FUNGAL TRANSCRIPTIONAL ACTIVATORS USEFUL IN METHODS FOR PRODUCING A POLYPEPTIDE 审中-公开
标题翻译：用于生产多肽的方法中的真菌转录激活剂
公开(公告)号：WO2006040312A2
公开(公告)日：2006-04-20
申请号：PCT/EP2005055145
申请日：2005-10-11
申请人： DSM IP ASSETS BV , PEIJ VAN NOEL NICOLAAS MARIA E , PARENICOVA LUCIE
发明人： PEIJ VAN NOEL NICOLAAS MARIA E , PARENICOVA LUCIE
IPC分类号： C12N15/31 , C07K14/37 , C12N15/67
CPC分类号： C12N15/80
摘要： The present invention relates to functional cDNA and genomic sequences encoding PrtT proteins, which have transcriptional activity on a protease promoter, to PrtT proteins and to their use. The invention further relates to two distinct types of filamentous fungal cells. Filamentous fungal cells are transformed to over-express these PrtT proteins: this type of filamentous fungus will be highly suited as protease producer. Alternatively, the endogenous prtT genes of filamentous fungal cells are inactivated: this type of filamentous fungus is highly suited for the production of any polypeptide native or not which is highly sensitive for protease degradation. The PrtT proteins of the invention provide means for identification of functional homologues in other species.
摘要翻译：本发明涉及编码PrtT蛋白质的功能性cDNA和基因组序列，其对蛋白酶启动子具有转录活性，并且涉及它们的用途。本发明还涉及两种不同类型的丝状真菌细胞。转化丝状真菌细胞以过表达这些PrtT蛋白：这种丝状真菌将非常适合作为蛋白酶生产者。或者，丝状真菌细胞的内源性prtT基因被灭活：这种类型的丝状真菌非常适合于生产对于蛋白酶降解非常敏感的天然或非天然的多肽。本发明的PrtT蛋白提供用于鉴定其他物种中的功能同系物的方法。

3. 发明申请

WO2007063133A3 GENES USEFUL FOR THE INDUSTRIAL PRODUCTION OF CITRIC ACID 审中-公开
标题翻译：有利于工业生产柠檬酸的基因
公开(公告)号：WO2007063133A3
公开(公告)日：2007-09-13
申请号：PCT/EP2006069218
申请日：2006-12-01
申请人： DSM IP ASSETS BV , BAUWELEERS HUGO MARC KAREL , GROESENEKEN DOMINIQUE ROBERT , PEIJ VAN NOEL NICOLAAS MARIA E
发明人： BAUWELEERS HUGO MARC KAREL , GROESENEKEN DOMINIQUE ROBERT , PEIJ VAN NOEL NICOLAAS MARIA E
IPC分类号： C07K14/38 , C12P7/48
CPC分类号： C12P7/48 , C07K14/38
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the (bio)synthesis of citric acid. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of citric acid from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for production of citric acid.
摘要翻译：本发明涉及编码参与柠檬酸（生物）合成的蛋白质的新鉴定的基因。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。本发明还涉及所述多核苷酸和多肽作为生物技术工具在从微生物生产柠檬酸中的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或所述微生物中发酵产物的生产效率。还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。本发明还涉及基因工程微生物及其生产柠檬酸的用途。

4. 发明申请

WO2008053018A2 IMPROVED PRODUCTION OF SECRETED PROTEINS BY FILAMENTOUS FUNGI 审中-公开
标题翻译：通过真菌真菌改良分泌的蛋白质的生产
公开(公告)号：WO2008053018A2
公开(公告)日：2008-05-08
申请号：PCT/EP2007061765
申请日：2007-10-31
申请人： DSM IP ASSETS BV , SAGT CORNELIS MARIA JACOBUS , VERRIPS CORNELIS THEODORUS , MULLER WALRAVEN HENRY , PEIJ VAN NOEL NICOLAAS MARIA E
发明人： SAGT CORNELIS MARIA JACOBUS , VERRIPS CORNELIS THEODORUS , MULLER WALRAVEN HENRY , PEIJ VAN NOEL NICOLAAS MARIA E
IPC分类号： C12N15/09
CPC分类号： C12P21/00 , C07K14/38 , C12N9/0004 , C12N9/10 , C12N9/12 , C12N9/16 , C12N9/2405 , C12N9/90 , C12N15/80
摘要： The present invention relates to a method to improve the secretion of a protein of interest by a filamentous fungal cell comprising inducing a phenotype in the cell selected from the group consisting of a lowered ERAD, an elevated UPR that does not induce an elevated ERAD, wherein ERAD preferably is lowered. The invention further relates to the filamentous fungal cell comprising the phenotype described above. The invention also relates to polynucleotides and polypeptides whose expression can be modulated in the filamentous fungal cell to obtain the above-described phenotype.
摘要翻译：本发明涉及一种改善丝状真菌细胞分泌目的蛋白质的方法，包括诱导细胞中的表型，所述细胞选自降低的ERAD，不诱导升高的ERAD的升高的UPR，其中 ERAD优选降低。本发明还涉及包含上述表型的丝状真菌细胞。本发明还涉及其表达可以在丝状真菌细胞中调节以获得上述表型的多核苷酸和多肽。

5. 发明申请

WO2005100573A9 FUNGAL PROMOTERS FOR EXPRESSING A GENE IN A FUNGAL CELL 审中-公开
标题翻译：用于在真菌细胞中表达基因的真菌促进剂
公开(公告)号：WO2005100573A9
公开(公告)日：2007-11-22
申请号：PCT/EP2005051696
申请日：2005-04-18
申请人： DSM IP ASSETS BV , WENZEL THIBAUT JOSE , PEIJ VAN NOEL NICOLAAS MARIA E , STAM HEIN
发明人： WENZEL THIBAUT JOSE , PEIJ VAN NOEL NICOLAAS MARIA E , STAM HEIN
IPC分类号： C12N15/80 , C07K14/38 , C07K14/47
CPC分类号： C07K14/38
摘要： The present invention relates to isolated fungal promoter DNA sequences, to DNA constructs, vectors, and fungal host cells comprising these promoters in operative association with coding sequences encoding polypeptides. The present invention also relates to methods for expressing a gene and/or producing a polypeptide using the new promoters isolated. The present invention also relates to methods for altering the transcription level and/or regulation of an endogenous gene using the new promoter of the invention.
摘要翻译：本发明涉及分离的真菌启动子DNA序列，DNA构建体，载体和包含这些启动子的真菌宿主细胞，其与编码多肽的编码序列有效结合。本发明还涉及使用分离的新启动子来表达基因和/或产生多肽的方法。本发明还涉及使用本发明的新启动子改变内源基因的转录水平和/或调节的方法。

6. 发明申请

WO2005100573A2 FUNGAL PROMOTERS FOR EXPRESSING A GENE IN A FUNGAL CELL 审中-公开
标题翻译：用于在真菌细胞中表达基因的真菌促进剂
公开(公告)号：WO2005100573A2
公开(公告)日：2005-10-27
申请号：PCT/EP2005051696
申请日：2005-04-18
申请人： DSM IP ASSETS BV , WENZEL THIBAUT JOSE , PEIJ VAN NOEL NICOLAAS MARIA E , STAM HEIN
发明人： WENZEL THIBAUT JOSE , PEIJ VAN NOEL NICOLAAS MARIA E , STAM HEIN
IPC分类号： C07K14/38 , C07K14/47 , C12N15/80
CPC分类号： C07K14/38
摘要： The present invention relates to isolated fungal promoter DNA sequences, to DNA constructs, vectors, and fungal host cells comprising these promoters in operative association with coding sequences encoding polypeptides. The present invention also relates to methods for expressing a gene and/or producing a polypeptide using the new promoters isolated. The present invention also relates to methods for altering the transcription level and/or regulation of an endogenous gene using the new promoter of the invention.
摘要翻译：本发明涉及分离的真菌启动子DNA序列，DNA构建体，载体和包含这些启动子的真菌宿主细胞，其与编码多肽的编码序列有效结合。本发明还涉及使用分离的新启动子来表达基因和/或产生多肽的方法。本发明还涉及使用本发明的新启动子改变内源基因的转录水平和/或调节的方法。

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