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1. 发明申请

WO2006133898A2 IMPROVED BIOSYNTHETIC PRODUCTION OF [5S,6S]-5,6-DIHYDROXYCYCLOHEXA-1,3-DIENE-1-CARBOXYLIC ACID (2,3-TRANS-CHD) 审中-公开
标题翻译： [5S，6S] -5,6-二羟基环己基-1,3-二苯基-1-羧酸（2,3-TRANS-CHD）的改进的生产生产方法
公开(公告)号：WO2006133898A2
公开(公告)日：2006-12-21
申请号：PCT/EP2006/005664
申请日：2006-06-13
申请人： DSM IP ASSETS B.V. , DSM BIOTECH GMBH , BONGAERTS, Johannes, Josef , RAEVEN, Leon, Jean, Renier, Marie , SPRENGER, Georg , KUHM, Andrea , TAKORS, Ralf , BUJNICKI, Robert, Piotr
发明人： BONGAERTS, Johannes, Josef , RAEVEN, Leon, Jean, Renier, Marie , SPRENGER, Georg , KUHM, Andrea , TAKORS, Ralf , BUJNICKI, Robert, Piotr
IPC分类号： C12P7/42
CPC分类号： C12P7/42
摘要： The invention relates to a process for the biosynthetic production of [5S,6S]-5,6-dihydroxy-cyclohexa-1 ,3-diene-1-carboxylic acid (2,3- trans -CHD) in an Escherichia microorganism having increased activity of isochorismate synthase and isochorismatase, and being deficient in activity of 2,3-dihydroxybenzoate synthase, wherein the process is carried out in a chorismate overproducing host microorganism also having decreased glucose dehydrogenase activity, or is carried out in a chorismate overproducing host microorganism naturally lacking glucose dehydrogenase activity. In this improved biosynthetic process, 2,3- trans -CHD is being produced in high yields and at concentrations in the biosynthetic reaction medium of at least 12 g/l. The invention further relates to the use of the supernatant of a process for the biosynthetic production of [5S,6S]-5,6-dihydroxy-cyclohexa-1 ,3-diene-1-carboxylic acid (2,3- trans -CHD) where the concentration of 2,3-trans-CHD in the process has arrived at a concentration of at least 12 g/l as a source for a co-factor for PQQ dependent enzymes.
摘要翻译：本发明涉及在具有增加的大肠杆菌中的[5S，6S] -5,6-二羟基 - 环己-1,3-二烯-1-羧酸（2,3-反式-CHD）的生物合成生产方法等位基因合成酶和异激酶活性，并且缺乏2,3-二羟基苯甲酸合成酶的活性，其中该方法在也具有降低的葡萄糖脱氢酶活性的过度生产的宿主微生物中进行，或者在过量产生宿主微生物的天竺葵中进行缺乏葡萄糖脱氢酶活性。在这种改进的生物合成过程中，以高产率和生物合成反应介质中的浓度产生至少12g / l的2,3-反式CHD。本发明还涉及生物合成生产[5S，6S] -5,6-二羟基 - 环己-1,3-二烯-1-羧酸（2,3-反式-CHD）的方法的上清液的用途），其中该过程中2,3-反式CHD的浓度已达到至少12g / l的浓度作为PQQ依赖性酶辅因子的来源。

2. 发明申请

WO2006128706A1 BIOSYNTHETIC PRODUCTION OF (5S,6S)-6-AMINO-5-HYDROXYCYCLOHEXA-1,3-DIENE-1-CARBOXYLIC ACID (2,3-CHA) 审中-公开
标题翻译：（5S，6S）-6-氨基-5-羟基环己基-1,3-二苯基-1-羧酸（2,3-CHA）的生产生产
公开(公告)号：WO2006128706A1
公开(公告)日：2006-12-07
申请号：PCT/EP2006/005237
申请日：2006-06-01
申请人： DSM IP ASSETS B.V. , BONGAERTS, Johannes Josef , MÜLLER, Michael , RAEVEN, Leon, Jean, Renier, Marie , LORBACH, Volker
发明人： BONGAERTS, Johannes Josef , MÜLLER, Michael , RAEVEN, Leon, Jean, Renier, Marie , LORBACH, Volker
IPC分类号： C12P13/04 , C07C229/48
CPC分类号： C12P13/04
摘要： The invention relates to a process for the biosynthetic production and recovery of [5S,6S]-6-amino-5-hydroxycyclohexa-1 ,3-diene-1-carboxylic acid) catalyzed, at increased enzyme activity, at least by an enzyme belonging to the class of aminodeoxyisochorismate (ADIC) synthases and an enzyme belonging to the class of isochorismatases, wherein (a) the production is carried out fermentatively with a suitable carbon source as starting material in a chorismate overproducing host microorganism, and with an aminodeoxyisochorismate (ADIC) synthase enzyme selected from the group of such ADIC synthase enzymes that are lacking ADIC lyase activity, and (b) the recovery is carried out by means of concentrating the supernatant of the fermentation to a 2,3-CHA concentration of at least 25 g/l, followed by crystallisation from the said concentrated supernatant. In a second embodiment, the present invention relates to the use of [5S,6S]-6-amino-5-hydroxycyclohexa-1 ,3-diene-1-carboxylic acid (2,3-CHA) as a catalytically active product, in particular as a chiral catalyst. Finally, the invention relates to the novel zinc complex of 2,3-CHA.
摘要翻译：本发明涉及至少通过酶催化生物合成生产和回收[5S，6S] -6-氨基-5-羟基环己-1,3- -1,3-二烯-1-羧酸的方法，其以增加的酶活性催化属于类别的氨基脱氧假单胞菌酸（ADIC）合酶和属于等位基因类别的酶，其中（a）以合适的碳源作为原料在发酵过氧化副产物宿主微生物中以及与氨基脱氧异乳清素 ADIC）合酶，其选自缺少ADIC裂解酶活性的ADIC合成酶组，和（b）通过将发酵的上清液浓缩至至少25的2,3-CHA浓度来进行回收 g / l，随后从所述浓缩的上清液中结晶。在第二个实施方案中，本发明涉及[5S，6S] -6-氨基-5-羟基环己-1,3-二烯-1-羧酸（2,3-CHA）作为催化活性产物的用途，特别是作为手性催化剂。最后，本发明涉及2,3-CHA的新型锌络合物。

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