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    • 5. 发明申请
    • MAIZE CYCLO1 GENE AND PROMOTER
    • MAIZE CYCLO1基因和促进剂
    • WO2006076189A2
    • 2006-07-20
    • PCT/US2006/000153
    • 2006-01-06
    • PIONEER HI-BRED INTERNATIONAL, INC.E. I. DU PONT DE NEMOURS & COMPANYDIEHN, ScottLU, Albert L.SIMS, Lynne E.WARD, Kim R.
    • DIEHN, ScottLU, Albert L.SIMS, Lynne E.WARD, Kim R.
    • C12N15/82C07K14/415A01H5/00
    • C12N15/8227C07K14/415C12N15/8279
    • The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Cyclo1. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence. The present invention also relates to isolated nucleic acids encoding plant cyclotides. The invention relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.
    • 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码Cyclo1的基因的根优选启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中,并再生表达核苷酸序列的稳定转化的植物。 本发明还涉及编码植物环肽的分离的核酸。 本发明涉及在正义或反义方向上构建编码全部或部分植物环肽的嵌合基因,其中嵌合基因的表达导致在转化的宿主细胞中产生改变水平的植物环肽。