专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO0120039A3 METHOD OF SEQUENCING A NUCLEIC ACID 审中-公开
标题翻译：测序核酸的方法
公开(公告)号：WO0120039A3
公开(公告)日：2002-03-21
申请号：PCT/US0025290
申请日：2000-09-15
申请人： CURAGEN CORP , ROTHBERG JONATHAN M , BADER JOEL S , DEWELL SCOTT B , MCDADE KEITH , SIMPSON JOHN W , BERKA JAN , COLANGELO CHRISTOPHER M
发明人： ROTHBERG JONATHAN M , BADER JOEL S , DEWELL SCOTT B , MCDADE KEITH , SIMPSON JOHN W , BERKA JAN , COLANGELO CHRISTOPHER M
IPC分类号： G01N33/50 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N21/05 , G01N21/17 , G01N33/15 , G01N33/53 , G01N33/566 , H04N5/225 , B01J19/00
CPC分类号： C12Q1/6837 , C12Q1/6827 , C12Q1/6869 , C12Q1/6874 , C12Q2531/125 , C12Q2535/101 , C12Q2565/301
摘要： Disclosed herein are methods and apparatuses for sequencing a nucleic acid. In one aspect, the method includes annealing a population of circular nucleic acid molecules to a plurality of anchor primers linked to a solid support, and amplifying those members of the population of circular nucleic acid molecules which anneal to the target nucleic acid, and then sequencing the amplified molecules by detecting the presence of a sequence by-product such as pyrophosphate.
摘要翻译：本文公开了用于测序核酸的方法和装置。一方面，该方法包括将多个环状核酸分子退火至与固体支持物连接的多个锚定引物，并扩增与靶核酸退火的环状核酸分子群体的那些成员，然后测序通过检测序列副产物如焦磷酸盐的存在来扩增分子。

2. 发明申请

WO9907896A3 DETECTION AND CONFIRMATION OF NUCLEIC ACID SEQUENCES BY USE OF OLIGONUCLEOTIDES COMPRISING A SUBSEQUENCE HYBRIDIZING EXACTLY TO A KNOWN TERMINAL SEQUENCE AND A SUBSEQUENCE HYBRIDIZING TO AN UNIDENTIFIED SEQUENCE 审中-公开
标题翻译：核苷酸序列的检测和确认通过使用包含随机杂交的寡核苷酸完全杂交到已知的终末序列和随机杂交到未经鉴别的序列
公开(公告)号：WO9907896A3
公开(公告)日：1999-04-29
申请号：PCT/US9816548
申请日：1998-08-07
申请人： CURAGEN CORP , ROTHBERG JONATHAN M , DEEM MICHAEL W , SIMPSON JOHN W
发明人： ROTHBERG JONATHAN M , DEEM MICHAEL W , SIMPSON JOHN W
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/68
CPC分类号： C12N15/1093 , C12Q1/6809 , C12Q1/6813 , C12Q1/6855 , C12Q2525/191 , C12Q2565/125
摘要： The present invention discloses a methodology which is directed to providing positive confirmation that nucleic acids, possessing putatively identified sequences predicted to generate observed GeneCalling signals, are actually present within the sample from which the signal was originally derived. The putatively identified nucleic acid fragment within the sample possesses 3'- and 5'-ends with known terminal subsequences, said method comprising: contacting said nucleic acid fragments in said sample in amplifying conditions with (i) a nucleic acid polymerase; (ii) "regular" primer oligonucleotides having sequences comprising hybridizable portions of said known terminal subsequences; and (iii) a "poisoning" oligonucleotide primer, said poisoning primer having a sequence comprising a first subsequence that is a portion of the sequence of one of said known terminal subsequences and a second subsequence that is a hybridizable portion of said putatively unidentified sequence which is adjacent to said one known terminal subsequence, wherein nucleic acids amplified with said poisoning primer are distinguishable upon detection from nucleic acids amplified with said nucleic acids amplified only with said regular primers; separating the products of the contacting step; and the detecting sequence is confirmed if the nucleic acids amplified with said poisoning primer are detected.
摘要翻译：本发明公开了一种旨在提供肯定确认的方法，核酸具有预测产生观察到的GeneCalling TM信号的推定鉴定序列实际上存在于最初得到信号的样本内。所述样品中的推测鉴定的核酸片段具有已知末端亚序列的3'和5'末端，所述方法包括：使扩增条件下的所述样品中的所述核酸片段与（i）核酸聚合酶; （ii）具有包含所述已知末端子序列的可杂交部分的序列的“规则”引物寡核苷酸; 所述中毒引物具有包含作为所述已知末端子序列之一序列的一部分的第一子序列和作为所述假定未鉴定序列的可杂交部分的第二子序列的序列，与所述一个已知末端子序列相邻，其中用所述中毒引物扩增的核酸在从用所述常规引物扩增的所述核酸扩增的核酸检测时是可区分的; 分离接触步骤的产物; 如果检测到用所述中毒引物扩增的核酸，则确认检测序列。

3. 发明申请

WO2007086935A8 METHODS OF AMPLIFYING AND SEQUENCING NUCLEIC ACIDS 审中-公开
标题翻译：放大和测序核酸的方法
公开(公告)号：WO2007086935A8
公开(公告)日：2008-05-15
申请号：PCT/US2006030235
申请日：2006-08-01
申请人： 454 LIFE SCIENCES CORP , MCDADE KEITH E , FIERRO JOSEPH M , KNIGHT JAMES R , CHARUMILIND JERRY , LEAMON JOHN H , MYERS EUGENE W , SIMPSON JOHN W , VOLKMER GREG A
发明人： MCDADE KEITH E , FIERRO JOSEPH M , KNIGHT JAMES R , CHARUMILIND JERRY , LEAMON JOHN H , MYERS EUGENE W , SIMPSON JOHN W , VOLKMER GREG A
IPC分类号： G06F19/00 , B01L3/00 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/68 , G01N21/25 , G01N21/64
CPC分类号： B01L3/502761 , B01L3/5027 , B01L3/5085 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G06F19/22
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
摘要翻译：本文提供了用于进行快速DNA测序的装置和方法，例如基因组测序。该方法包括以下步骤：制备用于基因组测序的样品DNA，以代表性方式扩增所制备的DNA，并仅在一个引物杂交步骤对经扩增的DNA进行多次测序反应。

4. 发明申请

WO2007086935A3 METHODS OF AMPLIFYING SEQUENCING NUCLEIC ACIDS 审中-公开
标题翻译：放大序列核酸的方法
公开(公告)号：WO2007086935A3
公开(公告)日：2007-11-15
申请号：PCT/US2006030235
申请日：2006-08-01
申请人： 454 LIFE SCIENCES CORP , MCDADE KEITH E , FIERRO JOSEPH M , KNIGHT JAMES R , CHARUMILIND JERRY , LEAMON JOHN H , MYERS EUGENE W , SIMPSON JOHN W , VOLKMER GREG A
发明人： MCDADE KEITH E , FIERRO JOSEPH M , KNIGHT JAMES R , CHARUMILIND JERRY , LEAMON JOHN H , MYERS EUGENE W , SIMPSON JOHN W , VOLKMER GREG A
IPC分类号： G06F19/00 , B01L3/00 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/68 , G01N21/25 , G01N21/64
CPC分类号： B01L3/502761 , B01L3/5027 , B01L3/5085 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G06F19/22
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
摘要翻译：本文提供了用于进行快速DNA测序的装置和方法，例如基因组测序。该方法包括以下步骤：制备用于基因组测序的样品DNA，以代表性方式扩增所制备的DNA，并仅在一个引物杂交步骤对经扩增的DNA进行多次测序反应。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式