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1. 发明申请

WO1997021800A2 RECOMBINANT HELIX MODIFICATION RECOGNITION PROTEINS AND USES THEREOF 审中-公开
标题翻译：重组HELIX修饰鉴定蛋白及其用途
公开(公告)号：WO1997021800A2
公开(公告)日：1997-06-19
申请号：PCT/US1996019018
申请日：1996-11-26
申请人： THOMAS JEFFERSON UNIVERSITY , CORNELL RESEARCH FOUNDATION, INC.
发明人： THOMAS JEFFERSON UNIVERSITY , CORNELL RESEARCH FOUNDATION, INC. , KMIEC, Eric, B. , HOLLOMAN, William, K. , GERHOLD, David
IPC分类号： C12N00/00
CPC分类号： C12Q1/6827 , C07K14/47
摘要： Disclosed are the Hmp class of polypeptides, DNA sequences encoding those polypeptides, and uses thereof, particularly in methods and kits for mismatch (for example, mutation) detection.
摘要翻译：公开了多肽的Hmp类，编码那些多肽的DNA序列及其用途，特别是用于错配（例如突变）检测的方法和试剂盒。

2. 发明申请

WO1998011214A1 MAMMALIAN AND HUMAN REC2 审中-公开
标题翻译：妈妈和人类REC2
公开(公告)号：WO1998011214A1
公开(公告)日：1998-03-19
申请号：PCT/IB1997001217
申请日：1997-09-11
申请人： THOMAS JEFFERSON UNIVERSITY , CORNELL RESEARCH FOUNDATION, INC.
发明人： THOMAS JEFFERSON UNIVERSITY , CORNELL RESEARCH FOUNDATION, INC. , HOLLOMAN, William, K. , RICE, Michael, C. , SMITH, Sheryl, T. , SHU, Zhigang , KMIEC, Eric, B.
IPC分类号： C12N15/12
CPC分类号： C12N9/00 , A01K2217/05
摘要： The invention concerns mammalian recombinase genes (REC2) and their promoters. Over expression of REC2 in a cell is found to facilitate homologous recombination, particularly homologous recombination using a DNA/RNA chimeric oligonucleotide and to sensitize a cell to the apoptotic effects of irradiation. The REC2 promoter, in combination with a strong enhancer, e.g., a SV40 enhancer, was found to be a strong promoter following irridiation of the cells. A radiation induceable promoter can be used to sensitize a cell to radiation treatment by operably linking the radiation induceable promoter to a gene whose expression converts a prodrug to a drug such as a herpes thymidine kinase gene.
摘要翻译：本发明涉及哺乳动物重组酶基因（REC2）及其启动子。发现细胞中REC2的过表达促进同源重组，特别是使用DNA / RNA嵌合寡核苷酸的同源重组，并使细胞对辐照的凋亡作用敏感。发现REC2启动子与强增强子（例如SV40增强子）组合，在细胞灌注后是强启动子。辐射诱导启动子可用于通过将辐射诱导启动子可操作地连接到表达将前药转化为药物如疱疹胸苷激酶基因的基因来使细胞对辐射治疗敏感。

3. 发明申请

WO1996022364A1 COMPOSITIONS AND METHODS TO PROMOTE HOMOLOGOUS RECOMBINATION IN EUKARYOTIC CELLS AND ORGANISMS 审中-公开
标题翻译：促进真核细胞和有机体中的同源重组的组合物和方法
公开(公告)号：WO1996022364A1
公开(公告)日：1996-07-25
申请号：PCT/US1996000265
申请日：1996-01-16
申请人： CORNELL RESEARCH FOUNDATION, INC. , THOMAS JEFFERSON UNIVERSITY
发明人： CORNELL RESEARCH FOUNDATION, INC. , THOMAS JEFFERSON UNIVERSITY , HOLLOMAN, William, K. , KMIEC, Eric, B.
IPC分类号： C12N09/00
CPC分类号： C12N9/00
摘要： The invention concerns genes encoding recombinases that can be used to promote homologous recombination in eukaryotic cells. The application teaches methods by which a recombinase of one species can be used to isolate a homologous recombinase of a different species and methods to identify the isolated homologs. Recombinases from Ustilago maydis and Saccharomyces cerevisiae are specifically included in the invention. The invention encompasses the method of producing an isolated recombinase protein in a prokaryotic cell and recovering the product in an active form. The invention also encompasses a genetically engineered gene which encodes a non-naturally occurring recombinase that causes a greater rate of recombination than does the naturally occurring recombinase. The invention further encompasses the use of recombinase proteins and of recombinase genes to promote homologous recombination, including recombination between a host cell genome and a chimeric oligonucleotide, i.e., an oligonucleotide having both RNA and DNA bases.
摘要翻译：本发明涉及编码可用于促进真核细胞中的同源重组的重组酶的基因。该申请教导了可以使用一种重组酶来分离不同物种的同源重组酶的方法，以及鉴定分离的同系物的方法。来自Ustilago maydis和Saccharomyces cerevisiae的重组酶特异性地包括在本发明中。本发明包括在原核细胞中产生分离的重组酶蛋白并以活性形式回收产物的方法。本发明还包括编码非天然存在的重组酶的遗传工程改造的基因，其导致比天然存在的重组酶更高的重组速率。本发明还包括使用重组酶蛋白和重组酶基因来促进同源重组，包括宿主细胞基因组和嵌合寡核苷酸之间的重组，即具有RNA和DNA碱基的寡核苷酸。

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