专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO0179548A3 METHOD OF DESIGNING ADDRESSABLE ARRAY FOR DETECTION OF NUCLEIC ACID SEQUENCE DIFFERENCES USING LIGASE DETECTION REACTION 审中-公开
标题翻译：设计可寻址阵列的方法，用于检测使用LIGASE检测反应的核酸序列差异
公开(公告)号：WO0179548A3
公开(公告)日：2003-02-06
申请号：PCT/US0110958
申请日：2001-04-04
申请人： CORNELL RES FOUNDATION INC , BARANY FRANCIS , ZIRVI MONIB , GERRY NORMAN P , FAVIS REYNA , KLIMAN RICHARD
发明人： BARANY FRANCIS , ZIRVI MONIB , GERRY NORMAN P , FAVIS REYNA , KLIMAN RICHARD
IPC分类号： G01N33/53 , C12M1/00 , C12N15/09 , C12Q1/25 , G01N33/569 , G01N37/00 , C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6827 , C12Q1/6874 , C12Q1/6886 , C12Q2527/107 , C12Q2521/501 , C12Q2521/319 , C12Q2565/501
摘要： The present invention is directed to a method of designing a plurality of capture oligonucleotide probes for use on a support to which complementary oligonucleotide probes will hybridize with little mismatch, where the plural capture oligonucleotide probes have melting temperatures within a narrow range. The first step of the method involves providing a first set of a plurality of tetramers of four nucleotides linked together, where (1) each tetramer within the set differs from all other tetramers in the set by at least two nucleotide bases, (2) no two tetramers within a set are complementary to one another, (3) no tetramers within a set are palindromic or dinucleotide repeats, and (4) no tetramer within a set has one or less or three or more G or C nucleotides. Groups of 2 to 4 of the tetramers from the first set are linked together to form a collection of multimer units. From the collection of multimer units, all multimer units formed from the same tetramer and all multimer units having a melting temperature in DEG C of less than 4 times the number of tetramers forming a multimer unit are removed to form a modified collection of multimer units. The modified collection of multimer units is arranged in a list in order of melting temperature. The order of the modified collection of multimer units is randomized in 2 DEG C increments of melting temperature.
摘要翻译：本发明涉及一种设计多个捕获寡核苷酸探针的方法，所述多个捕获寡核苷酸探针用于互补寡核苷酸探针与少量错配杂交的载体上，其中多个捕获寡核苷酸探针具有在窄范围内的熔融温度。该方法的第一步包括提供连接在一起的四个核苷酸的多个四聚体的第一组，其中（1）组中的每个四聚体与组中的所有其它四聚体不同，至少两个核苷酸碱基，（2）否一组内的两个四聚体彼此互补，（3）一组内的四聚体没有回归或二核苷酸重复，以及（4）组内的四聚体没有一个或多个或三个或更多个G或C核苷酸。来自第一组的4至4个四聚体的组连接在一起以形成多聚体单元的集合。从多聚体单元的收集中，除去由相同的四聚体形成的所有多聚体单元和具有小于组成多聚体单元的四聚体数目的4倍的熔点的聚合温度的所有多聚体单元以形成多聚体单元的修改的集合。修改的多聚体单元的集合按照熔融温度的顺序排列在列表中。多聚体单元的修改收集的顺序是以2℃的熔融温度增量随机化。

IPRDB

热门服务

关于我们

友情链接

联系方式