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1. 发明申请

WO2011035105A1 REDUCED CODON MUTAGENESIS 审中-公开
标题翻译：减少CODON MUTAGENESIS
公开(公告)号：WO2011035105A1
公开(公告)日：2011-03-24
申请号：PCT/US2010/049251
申请日：2010-09-17
申请人： CODEXIS, INC. , FOX, Richard , GIVER, Lorraine J. , HELD, Daniel , HATTENDORF, Douglas , CHOUDHARY, Trish
发明人： FOX, Richard , GIVER, Lorraine J. , HELD, Daniel , HATTENDORF, Douglas , CHOUDHARY, Trish
IPC分类号： C40B50/06 , C40B40/06 , C12P19/34
CPC分类号： C12N15/102 , C12N15/1027 , C40B40/06 , C40B50/06
摘要： Methods and compositions that reduce complexity of libraries of variant biological molecules, that reduce oversampling of these libraries during screening and that improve screening efficiency are provided. Sets of efficient degenerate codon sets are provided that efficiently encode all, or nearly all canonical amino acids. Degenerate oligonucleotides comprising these codons are provided, as are polynucleotide variants. Variant pooling strategies are used during library construction. Logical filtering is applied to select codon sites for mutagenesis, or to select amino acid sets to be incorporated at such sites. Methods for reducing non-optimal oversampling during screening are provided.
摘要翻译：提供了减少变体生物分子的文库的复杂性的方法和组合物，可以减少筛选过程中这些文库的过采样并提高筛选效率。提供了有效地编码所有或几乎所有规范氨基酸的有效简并密码子集的集合。提供了包含这些密码子的简并寡核苷酸，以及多核苷酸变体。图书馆建设期间采用变式汇总策略。应用逻辑过滤来选择用于诱变的密码子位点，或选择要在这些位点掺入的氨基酸组。提供了在筛选期间减少非最佳过采样的方法。

2. 发明申请

WO2013003290A1 PROTEIN VARIANT GENERATION BY REGION SHUFFLING 审中-公开
标题翻译：蛋白质变异区域产生
公开(公告)号：WO2013003290A1
公开(公告)日：2013-01-03
申请号：PCT/US2012/044070
申请日：2012-06-25
申请人： CODEXIS, INC. , CLARK, Louis , CHOUDHARY, Trish
发明人： CLARK, Louis , CHOUDHARY, Trish
IPC分类号： C40B10/00 , C12N15/11
CPC分类号： C12N15/1034 , C12N9/0004 , C12N9/24 , C12N9/2434 , C12N15/1027 , C12P19/34
摘要： Region shuffling methods for efficiently introducing diversity and exploring sequence space are described. Libraries produced directly from these methods contain high fractions of protein variants harboring multiple beneficial mutations. Typically, the methods produce these variants efficiently without the need for sequencing beneficial mutants identified at intermediate stages of the process.
摘要翻译：描述了有效引入分集和探索序列空间的区域混洗方法。直接从这些方法生产的文库含有高分数的含有多个有益突变的蛋白质变体。通常，所述方法有效地产生这些变体，而不需要测序在该过程的中间阶段鉴定的有益突变体。

3. 发明申请

WO2015026747A1 SEPARATING AND DEMINERALIZING BIOMOLECULE SOLUTIONS BY ELECTRODIALYSIS 审中-公开
标题翻译：电生物分离和分解生物分子解决方案
公开(公告)号：WO2015026747A1
公开(公告)日：2015-02-26
申请号：PCT/US2014/051564
申请日：2014-08-18
申请人： CHOUDHARY, Trish
发明人： CHOUDHARY, Trish
IPC分类号： B01D61/44
CPC分类号： B01D61/44 , A23C9/146 , A23C21/00 , A23J1/205 , B01D61/027 , B01D61/145 , B01D61/147 , B01D61/445 , B01D61/46 , B01D61/48 , B01D61/52 , B01D61/58 , B01D65/08 , B01D2311/04 , B01D2311/2688 , B01D2321/223
摘要： Methods and systems are provided for separation and/or purification of biomolecule(s) from a solution and for demineralization of a solution containing biomolecule(s) and ion(s). The methods and systems described herein include one or more ion exchange membrane(s), (e.g., ion exchange membrane(s) capable of water splitting), in an electrochemical cell, from which bound biomolecule(s) or ion(s) may be recovered from the membrane and separated from other components present in the solution by reversing the polarity of the electrodes. Using methods and systems described herein, biomolecules (e.g., whey proteins and lactose), and ions (e.g., milk salts), are able to be separated and recovered in their native forms with high biological value as premium food grade products. Methods and systems described herein offer a significant advantage over traditional processes used (e.g., in the food and beverage industry), for cost effective and chemical-free processing/extraction of valuable products from complex solutions such as whey.
摘要翻译：提供了用于从溶液中分离和/或纯化生物分子和用于含有生物分子和离子的溶液的软化的方法和系统。本文描述的方法和系统包括一个或多个离子交换膜（例如，能够水分解的离子交换膜），在电化学电池中，结合的生物分子或离子可以通过反转电极的极性从膜中回收并与溶液中存在的其它成分分离。使用本文所述的方法和系统，生物分子（例如乳清蛋白和乳糖）和离子（例如乳盐）能够以具有高生物价值的天然形式分离和回收，作为高级食品级产品。与所使用的传统方法（例如，在食品和饮料工业中）相比，方法和系统具有显着的优点，用于从诸如乳清的复杂溶液中进行成本有效和无化学处理/提取有价值的产物。

4. 发明申请

WO2014015278A1 PRODUCTION OF FATTY ALCOHOLS FROM ENGINEERED MICROORGANISMS 审中-公开
标题翻译：从工程微生物生产脂肪醇
公开(公告)号：WO2014015278A1
公开(公告)日：2014-01-23
申请号：PCT/US2013/051340
申请日：2013-07-19
申请人： CODEXIS, INC.
发明人： VROOM, Jonathan , BALATSKAYA, Svetlana , BARAK, Yoram , CLARK, Louis , CHOUDHARY, Trish , KARLSHOEJ, Kristian , VALLE, Fernando , CHAN, Kaman
IPC分类号： C07C29/00 , C07C31/00 , C07C31/02 , C07C33/00 , C07C33/02 , C07C43/00
CPC分类号： C12P7/64 , A61K8/342 , A61K2800/85 , C11D3/2013 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/1007 , C12N9/1029 , C12N9/16 , C12N9/88 , C12N9/93 , C12P7/04 , C12Y101/01 , C12Y101/011 , C12Y103/01009 , C12Y103/99 , C12Y201/01005 , C12Y203/01029 , C12Y203/01041 , C12Y203/01086 , C12Y203/0118 , C12Y301/02 , C12Y301/02001 , C12Y301/02027 , C12Y402/01059 , C12Y602/01 , C12Y602/01003
摘要： Recombinant microorganisms are provided which have been engineered to produce fatty alcohols. Also provided are recombinant microorganisms which comprise a heterologous polynucleotide encoding a fatty alcohol reductase enzyme and an introduced polynucleotide encoding a β-ketoacyl acyl carrier protein synthase.
摘要翻译：提供了重组微生物，其已被工程化以生产脂肪醇。还提供了包含编码脂肪醇还原酶的异源多核苷酸和编码β-酮酰酰基载体蛋白合酶的引入的多核苷酸的重组微生物。

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