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1. 发明申请

WO1997012045A1 METHOD OF MAKING A PROTEASE DEFICIENT HOST 审中-公开
标题翻译：制备抗体缺陷性宿主的方法
公开(公告)号：WO1997012045A1
公开(公告)日：1997-04-03
申请号：PCT/US1996014947
申请日：1996-09-17
申请人： CHIRON CORPORATION , BISHOP, Robert, J. , INNIS, Michael, A.
发明人： CHIRON CORPORATION
IPC分类号： C12N15/67
CPC分类号： C12N15/67 , C12N9/50
摘要： This invention relates to improved methods of recombinant protein production in a variety of host cells including yeast and bacteria. The host cells herein are deficient in protease activity, but the deficiency is not a result of a defective protease structural gene.
摘要翻译：本发明涉及在多种宿主细胞（包括酵母和细菌）中重组蛋白质生产的改进方法。本文中的宿主细胞缺乏蛋白酶活性，但缺陷不是蛋白酶结构基因缺陷的结果。

2. 发明申请

WO1997012044A3 PICHIA SECRETORY LEADER FOR PROTEIN EXPRESSION 审中-公开
公开(公告)号：WO1997012044A3
公开(公告)日：1997-04-03
申请号：PCT/US1996015329
申请日：1996-09-25
申请人： CHIRON CORPORATION , CRAWFORD, Kenneth , ZAVOR, Isabel , BISHOP, Robert, J. , INNIS, Michael, A.
发明人： CHIRON CORPORATION
IPC分类号： C12N15/81

3. 发明申请

WO1998021232A2 MUTANT FORMS OF FAS LIGAND AND USES THEREOF 审中-公开
标题翻译： FAS配偶的突变形式及其用途
公开(公告)号：WO1998021232A2
公开(公告)日：1998-05-22
申请号：PCT/US1997020864
申请日：1997-11-13
申请人： CHIRON CORPORATION
发明人： CHIRON CORPORATION , CHU, Keting
IPC分类号： C07K14/00
CPC分类号： C07K14/70575 , A61K38/00 , C07K2319/00 , C12N2799/021 , C12N2799/022 , C12N2799/027
摘要： The invention provides for DNA encoding Fas ligand muteins and chimeras and the proteins encoded thereby. The invention further includes the use of DNA and vectors to produce transformed cells expressing the mutant or chimeric Fas ligand. When the Fas ligand of the invention is a non cleavable form, the cells expressing the Fas ligand are useful in vitro for identifying Fas expressing cells and in vitro or in vivo for reducing populations of Fas expressing cells. Thus, in other embodiments, the present invention is also directed to a method for treating a patient, for example a mammal, for autoimmune disease or transplant rejection by administering a Fas ligand therapeutic agent. The therapeutic agent is a polypeptide, a polynucleotide encoding the polypeptide or a small molecule. The polypeptides include full-length Fas ligand polypeptide, or a biologically active variant, derivative, portion, fusion or peptide thereof.
摘要翻译：本发明提供编码Fas配体突变蛋白和嵌合体的DNA及其编码的蛋白质。本发明还包括使用DNA和载体产生表达突变体或嵌合Fas配体的转化细胞。当本发明的Fas配体是不可切割的形式时，表达Fas配体的细胞在体外可用于鉴定Fas表达细胞，并在体外或体内用于减少Fas表达细胞的群体。因此，在其它实施方案中，本发明还涉及通过施用Fas配体治疗剂来治疗患者，例如哺乳动物用于自身免疫疾病或移植排斥的方法。治疗剂是多肽，编码多肽的多核苷酸或小分子。多肽包括全长Fas配体多肽或其生物活性变体，衍生物，部分，融合物或肽。

4. 发明申请

WO1998018550A1 TRANSPONDER TAGGING OF CONSTITUENTS USED IN COMPOUND SYNTHESIS 审中-公开
标题翻译：复合物合成中使用的组分标签
公开(公告)号：WO1998018550A1
公开(公告)日：1998-05-07
申请号：PCT/US1997019705
申请日：1997-10-29
申请人： CHIRON CORPORATION
发明人： CHIRON CORPORATION , GIGER, Rudolf, Karl, A. , MATTES, Henri , BRAY, Andrew, Malcolm , MAEJI, Nobuyoshi, Joe
IPC分类号： B01J19/00
CPC分类号： G06K19/04 , B01J19/0046 , B01J2219/00463 , B01J2219/00504 , B01J2219/00506 , B01J2219/00545 , B01J2219/00547 , B01J2219/00567 , B01J2219/0059 , B01J2219/00592 , B01J2219/00596 , B01J2219/00689 , C40B70/00 , Y10T436/13
摘要： A synthesis member (10) preferably comprises two elements: a crown (12) and a stem (14). The stem (14) includes a tag, such as a transponder (16), that is used to identify the synthesis member (10) and/or the synthetic history of the synthesis member (10) when used in automated synthesis of compound libraries containing large numbers (e.g. 1000 or more) compounds. The crown (12) provides the location at which compounds are synthesized. The combination of a crown (12) for use with compound synthesis and stem allows the tracking of individual synthesis member (10) from a library containing thousands of such synthesis members (10). Because each synthesis member (10) is individually tracked and can be logged into a database and/or process flow control system, the synthesis of several thousand individual compounds in conventional containers, such as round bottom flask is made possible using only traditional compound synthesis approaches.
摘要翻译：合成构件（10）优选地包括两个元件：冠部（12）和杆（14）。当用于自动合成含有化合物的化合物文库时，茎（14）包括标签，例如应答器（16），其用于鉴定合成成员（10）和/或合成成员（10）的合成历史大量（如1000个或更多）化合物。表冠（12）提供化合物合成的位置。与化合物合成和茎一起使用的冠（12）的组合允许从包含数千种这样的合成成员（10）的文库追踪个体合成成员（10）。因为每个合成构件（10）被单独地跟踪并且可以被记录到数据库和/或工艺流程控制系统中，所以可以使用仅传统的复合合成方法在常规容器（例如圆底烧瓶）中合成数千个单独的化合物。

5. 发明申请

WO1998007858A1 HUMAN POLYHOMEOTIC 1(hphl) ACTS AS A TUMOR SUPPRESSOR 审中-公开
标题翻译：人体聚合物1（hphl）ACTS作为肿瘤抑制剂
公开(公告)号：WO1998007858A1
公开(公告)日：1998-02-26
申请号：PCT/US1997014866
申请日：1997-08-22
申请人： CHIRON CORPORATION
发明人： CHIRON CORPORATION , RANDAZZO, Filippo
IPC分类号： C12N15/12
CPC分类号： C07K14/4702 , A61K38/00 , C07K2319/00
摘要： A novel human tumor suppressor gene termed polyhomeotic 1 is disclosed. The human polyhomeotic 1 gene and protein can be used as therapeutic and diagnostic tools for proliferative and developmental disorders. The gene can also be used to identify a p13 region of human chromosome 12.
摘要翻译：公开了一种称为聚合1的新型人肿瘤抑制基因。人类多灶性1基因和蛋白质可用作增殖和发育障碍的治疗和诊断工具。该基因也可用于鉴定人染色体12的p13区域。

6. 发明申请

WO1997044469A2 MULTIPLE EPITOPE FUSION PROTEIN 审中-公开
标题翻译：多功能融合蛋白融合蛋白
公开(公告)号：WO1997044469A2
公开(公告)日：1997-11-27
申请号：PCT/US1997008950
申请日：1997-05-23
申请人： CHIRON CORPORATION
发明人： CHIRON CORPORATION , VALENZUELA, Pablo, D., T. , CHIEN, David, Ying
IPC分类号： C12N15/62
CPC分类号： C07K14/005 , C07K2319/00 , C12N2770/24222
摘要： Multiple copy epitope immunoassays are produced by: (1) identifying nucleotide sequences that encode a plurality of different epitopes; (2) placing the nucleotide sequences into an expression cassette wherein at least two copies of a sequence coding for the same epitope, preferably from different strains of a pathogen, are placed in the cassette; (3) transforming a suitable host with the cassette in order to express the sequences encoding the epitopes; (4) purifying the expressed epitopes; and (5) coating the epitopes on a surface of a substrate. The purified epitopes are encompassed by the general structural formula (A)x-(B)y-(C)z which represents a linear amino acid sequence, B is an amino acid sequence of an epitope or cluster of epitopes and each B contains at least five and not more than 1,000 amino acids, y is an integer of 2 or more, A and C are each independently an amino acid sequence of an epitope or cluster of epitopes not adjacent to B in nature and x and z are each independently an integer of 0 or more wherein at least one of x and z is 1 or more. The epitopes of the invention are more soluble than and are therefore more easily purified than conventional epitopes. Further, the presence of repeating epitope sequences (repeating at least B in the same linear amino acid sequence from different strains of a pathogen) increases the sensitivity and specificity of the assay. Repeated epitope sequences in a single linear antigen also decreases masking problems and makes it possible to include a greater number of epitopes on a unit area of substrate thereby improving sensitivity in the detection of antibodies.
摘要翻译：多拷贝表位免疫测定通过以下方式产生：（1）鉴定编码多个不同表位的核苷酸序列; （2）将核苷酸序列置于表达盒中，其中编码相同表位，优选来自病原体的不同菌株的序列的至少两个拷贝被放置在盒中; （3）用盒子转化合适的宿主以表达编码表位的序列; （4）纯化表达的表位; 和（5）在基材的表面上涂覆表位。纯化的表位被代表线性氨基酸序列的一般结构式（A）x-（B）y-（C）z所涵盖，B是表位或表位簇的氨基酸序列，每个B含有至少5个且不超过1,000个氨基酸，y是2或更大的整数，A和C各自独立地是本质上不与B相邻的表位的表位或簇的氨基酸序列，x和z各自独立地为 0以上的整数，x和z中的至少一个为1以上。本发明的表位比常规表位更可溶，因此更容易纯化。此外，重复表位序列的存在（在来自不同病原体的菌株的同一线性氨基酸序列中至少重复B）增加了测定的灵敏度和特异性。单一线性抗原中的重复表位序列也减少掩蔽问题，并且可以在底物的单位面积上包含更多数目的表位，从而提高抗体检测的灵敏度。

7. 发明申请

WO1997042338A1 CROSSLESS RETROVIRAL VECTORS 审中-公开
标题翻译：无缝回归矢量
公开(公告)号：WO1997042338A1
公开(公告)日：1997-11-13
申请号：PCT/US1997007697
申请日：1997-05-05
申请人： CHIRON CORPORATION
发明人： CHIRON CORPORATION , RESPESS, James, G. , DePOLO, Nicholas, J. , CHADA, Sunil , SAUTER, Sybille , BODNER, Mordechai , DRIVER, David, A.
IPC分类号： C12N15/86
CPC分类号： C12N15/86 , C12N2740/13052
摘要： Retroviral vector constructs are described which have a 5' LTR, a tRNA binding site, a packaging signal, one or more heterologous sequences, an origin of second strand synthesis and a 3' LTR, wherein the vector construct lacks retroviral gag/pol or env coding sequences. In addition, gag/pol, and env expression cassettes are described wherein the expression cassettes lack a consecutive sequence of more than 8 nucleotides in common. The above-described retroviral vector constructs, gap/pol and env expression cassettes may be utilized to construct producer cell lines which preclude the formation of replication competent virus.
摘要翻译：描述了具有5'LTR，tRNA结合位点，包装信号，一个或多个异源序列，第二链合成起始和3'LTR的逆转录病毒载体构建体，其中载体构建体缺乏逆转录病毒gag / pol或env 编码序列。此外，描述了gag / pol和env表达盒，其中表达盒缺少共同超过8个核苷酸的连续序列。上述逆转录病毒载体构建体，gap / pol和env表达盒可用于构建排除形成复制能力的病毒的生产细胞系。

8. 发明申请

WO1997042219A1 MAMMALIAN ADDITIONAL SEX COMBS (MAMMALIAN Asx) ACTS AS A TUMOR SUPPRESSOR 审中-公开
标题翻译： MAMMALIAN ADDITIONAL SEX COMBS（MAMMALIAN ASX）ACTS AS TUMOR SUPPRESSOR
公开(公告)号：WO1997042219A1
公开(公告)日：1997-11-13
申请号：PCT/US1997007805
申请日：1997-05-08
申请人： CHIRON CORPORATION
发明人： CHIRON CORPORATION , RANDAZZO, Filippo
IPC分类号： C07K05/00
CPC分类号： C07K14/47 , A61K38/00 , C07H21/04
摘要： Mammalian Asx gene and amino acid sequences encoded by the mammalian Asx gene, are described. The mammalian Asx gene and gene products are useful for diagnostic and therapeutic applications in proliferative and developmental disorders. Modulators of mammalian Asx can be identified using the disclosed genes. The modulators can be used in the context of cancer therapy or a treatment of a developmental disorder. Asx is also useful for inducing differentiation in a population of progenitor cells.
摘要翻译：描述了由哺乳动物Asx基因编码的哺乳动物Asx基因和氨基酸序列。哺乳动物Asx基因和基因产物可用于增殖和发育障碍中的诊断和治疗应用。使用公开的基因可以鉴定哺乳动物Asx的调节剂。调节剂可用于癌症治疗或发展障碍治疗的上下文中。 Asx也可用于诱导祖细胞群体的分化。

9. 发明申请

WO1997035969A3 PEPTIDE LIGANDS OF THE UROKINASE RECEPTOR 审中-公开
公开(公告)号：WO1997035969A3
公开(公告)日：1997-10-02
申请号：PCT/US1997005199
申请日：1997-03-28
申请人： CHIRON CORPORATION , BRIGHAM AND WOMEN'S HOSPITAL , ROSENBERG, Steven , DOYLE, Michael , CHAPMAN, Harold, A.
发明人： CHIRON CORPORATION , BRIGHAM AND WOMEN'S HOSPITAL
IPC分类号： C12N15/10

10. 发明申请

WO1997030155A1 GENE THERAPY METHOD USING FGF-5 审中-公开
标题翻译：使用FGF-5的基因治疗方法
公开(公告)号：WO1997030155A1
公开(公告)日：1997-08-21
申请号：PCT/US1997002338
申请日：1997-02-12
申请人： CHIRON CORPORATION
发明人： CHIRON CORPORATION , GOSPODAROWICZ, Denis
IPC分类号： C12N15/12
CPC分类号： C07K14/50 , A61K48/00 , C12N2799/021 , C12N2799/027
摘要： A method is described for introducing an FGF-5 nucleic acid sequence into a mammalian host cell. The FGF-5 nucleic acid sequence lacks the signal sequence so that cells that are transformed with the sequence will not become tumirogenic. It is intended that the FGF-5 sequence is introduced into mammalian cells to promote angiogenesis. Preferably, the FGF-5 sequence is introduced into a human patient to treat myocardial ischemia or peripheral vascular disease.
摘要翻译：描述了将FGF-5核酸序列引入哺乳动物宿主细胞的方法。 FGF-5核酸序列缺少信号序列，使得用该序列转化的细胞不会变成肿瘤发生。旨在将FGF-5序列引入哺乳动物细胞以促进血管发生。优选地，将FGF-5序列引入人类患者以治疗心肌缺血或外周血管疾病。

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