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1. 发明申请

WO1996013606A1 SEQUENCE ANALYSIS OF SACCHARIDE MATERIAL 审中-公开
标题翻译：食品材料的序列分析
公开(公告)号：WO1996013606A1
公开(公告)日：1996-05-09
申请号：PCT/GB1995002541
申请日：1995-10-30
申请人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED , GALLAGHER, John, Thomas , TURNBULL, Jeremy, Ewan , HOPWOOD, John, Joseph
发明人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED
IPC分类号： C12Q01/34
CPC分类号： C12Q1/34 , G01N2333/924 , G01N2400/40
摘要： In a method of analysing saccharide material such as glycosaminoglycans (GAG's) to determine the sequence of monosaccharide units in oligosaccharide chains thereof, the saccharide chains are end referenced, e.g. by labelling or tagging at their reducing ends, and the saccharide material is subjected to a controlled partial depolymerisation using a selective scission reagent, for example low pH nitrous acid, which cleaves internal glycosidic linkages in accordance with a known linkage specificity so as to produce a mixed set of saccharide chain fragments having different lengths ranging throughout the full spectrum of possible lengths for the particular glycosidic linkage specificity of the selective scission reagent employed. Samples of the mixed set of saccharide chain fragments are then treated with selected exoenzymes including exoglycosidases that cleave only particular glycosidic linkages at the non-reducing end of saccharide chains and exosulphatases that selectively remove sulphate groups from monosaccharide residues at the non-reducing end of saccharide chains. These exoenzymes are applied to the samples either singly or in combination in accordance with a predetermined strategy. The treated samples are then analysed, conveniently by polyacrylamide gel electrophoresis (PAGE) to detect the chain fragments present which have a reducing end derived from the reducing end of the corresponding chain in the original saccharide material. Using PAGE, preferably gradient PAGE, the different samples are run in parallel tracks of the gel and a migration banding pattern is obtained that provides information which enables the monosaccharide sequence in the original saccharide material to be deduced.
摘要翻译：在分析诸如糖胺聚糖（GAG）等糖类物质以确定其糖链中的单糖单元序列的方法中，糖链为末端参考，例如，通过在其还原末端标记或标记，并且使用选择性切断试剂（例如低pH亚硝酸）对糖类物质进行受控的部分解聚，所述选择性裂解试剂根据已知的连锁特异性切割内部糖苷键，从而产生具有不同长度的混合组的糖链片段可以在所用选择性分裂试剂的特定糖苷键特异性的整个可能长度的全谱范围内。混合的糖链片段的样品然后用选择的外切酶处理，所述外切酶包括外切酶，其仅在糖链的非还原末端处切割特定的糖苷键，并且在糖的非还原末端选择性地从单糖残基中除去硫酸根基团链。这些外酶根据预定的策略单独或组合应用于样品。然后通过聚丙烯酰胺凝胶电泳（PAGE）方便地分析经处理的样品，以检测存在的链段，其具有衍生自原始糖类材料中相应链的还原末端的还原末端。使用PAGE，优选梯度PAGE，不同的样品在凝胶的平行轨道中运行，并且获得提供能够推导原始糖类材料中的单糖序列的信息的迁移条带图案。

2. 发明申请

WO1993019096A1 OLIGOSACCHARIDES HAVING GROWTH FACTOR BINDING AFFINITY 审中-公开
标题翻译：具有生长因子结合亲和力的低聚糖
公开(公告)号：WO1993019096A1
公开(公告)日：1993-09-30
申请号：PCT/GB1993000597
申请日：1993-03-23
申请人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED , GALLAGHER, John, Thomas , TURNBULL, Jeremy, Ewan
发明人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED
IPC分类号： C08B37/10
CPC分类号： C07H3/06 , C08B37/0078
摘要： Oligosaccharides having a high specific binding affinity for FGF growth factors and made up of less than ten disaccharide units in all are disclosed which include sulphated disaccharide units composed of an N-sulphated glucosamine residue and a 2-0-sulphated iduronic acid residue. A method is also disclosed for preparing these oligosaccharides in a purified and relatively homogeneous state from glycosaminoglycans such as heparan sulphate. For the best FGF-binding affinity there are preferably at least four of the sulphated disaccharide units arranged as an internal contiguous sequence. The most favoured structures contain fourteen monosaccharide residues in all, but structures having twelve monosaccharide residues can also have quite high FGF-binding affinity, at least for bFGF. These oligosaccharides can either activate and stimulate FGF activity or inhibit FGF activity, and uses thereof as drugs for therapeutic purposes in medicine are also disclosed.

3. 发明申请

WO1994021689A1 HEPARAN SULPHATE OLIGOSACCHARIDES HAVING HEPATOCYTE GROWTH FACTOR BINDING AFFINITY 审中-公开
标题翻译：具有肝细胞生长因子结合亲和力的HEPARAN SULPHATE OLIGOSACCHARIDES
公开(公告)号：WO1994021689A1
公开(公告)日：1994-09-29
申请号：PCT/GB1994000615
申请日：1994-03-24
申请人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED , LYON, Malcolm , GALLAGHER, John, Thomas
发明人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED
IPC分类号： C08B37/10
CPC分类号： C07H3/06 , C08B37/0078
摘要： Oligosaccharides having a high specific binding affinity for HGF growth factors and made up of relatively low molecular weight sulphated oligosaccharide chains are disclosed. The chains are composed of at least three disaccharide units including one or more internal sequences of an N-sulphated D-glucosamine 6-sulphate residue and an L-iduronic acid residue. A method is also disclosed for preparing these oligosaccharides in a purified and relatively homogeneous state from heparan sulphate. For the best HGF-binding affinity there are preferably at least five disaccharide units. The most favoured structures contain twelve or fourteen monosaccharide residues in all and include a relatively high proportion of 6-0-sulphated hexosamines, e.g. more than 30 % or even 50 %, as compared to oligosaccharide chains of unmodified native heparan sulphate. These oligosaccharides can modulate HGF activity, and uses thereof as drugs for therapeutic purposes in medicine are also disclosed.
摘要翻译：公开了对相对低分子量硫酸化糖链构成的对HGF生长因子具有高特异性结合亲和力的低聚糖。链由至少三个二糖单元组成，包括N-硫酸化D-葡糖胺-6-硫酸酯残基和L-艾杜糖醛酸残基的一个或多个内部序列。还公开了一种制备来自硫酸乙酰肝素的纯化且相对均匀的这些寡糖的方法。为了获得最佳的HGF结合亲和力，优选至少5个二糖单位。最有利的结构总共含有十二个或十四个单糖残基，并且包括相对高比例的6-0-硫酸化己糖胺，例如。与未修饰的天然硫酸乙酰肝素的寡糖链相比，超过30％或甚至50％。这些寡糖可以调节HGF活性，并且也公开了其作为药物治疗用途的药物的用途。

4. 发明申请

WO1998008973A1 GLOBAL AMPLIFICATION OF NUCLEIC ACIDS 审中-公开
标题翻译：核酸的全球放大
公开(公告)号：WO1998008973A1
公开(公告)日：1998-03-05
申请号：PCT/GB1997002320
申请日：1997-08-29
申请人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED , HAMPSON, Ian, Noel , HAMPSON, Lynne
发明人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED
IPC分类号： C12Q01/68
CPC分类号： C12N15/1096 , C12Q1/686 , C12Q2600/158 , C12Q2565/518 , C12Q2525/179
摘要： Nucleic acid starting material composed of a collection of single stranded nucleic acid molecules is anchored and processed by a directional random priming method to produce a mixture of shorter random size DNA molecules well-suited for achieving substantially uniform global PCR amplification. The processing and global amplification method disclosed is especially useful in conjunction with subtractive hybridization procedures applied, for example, to the study of differential gene expression.
摘要翻译：由单链核酸分子的集合组成的核酸起始材料通过定向随机引发方法进行锚定和加工，以产生适合于实现基本上均匀的全局PCR扩增的较短随机大小的DNA分子的混合物。所公开的加工和全局扩增方法特别适用于应用于例如差异基因表达研究的消减杂交程序。

5. 发明申请

WO1996026932A1 2-ARYLBENZAZOLE COMPOUNDS 审中-公开
标题翻译： 2-氨基苯并噻唑化合物
公开(公告)号：WO1996026932A1
公开(公告)日：1996-09-06
申请号：PCT/GB1996000440
申请日：1996-02-28
申请人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED , STEVENS, Malcolm, Francis, Graham , SHI, Dong-Fang , BRADSHAW, Tracey, Dawn , WRIGLEY, Samantha
发明人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED
IPC分类号： C07D277/66
CPC分类号： C07D263/57 , C07D277/66
摘要： There are disclosed herein 2-phenylbenzazole compounds having a 3'-substituent and a 4'-NR R substituent in the phenyl group where R and R are each hydrogen or alkyl, or where the 4'-NR R substituent is N-acyl (or N-benzoyl). There are also disclosed 2-phenylbenzazole compounds in the form of 4'-N sulphamate salts. Such compounds exhibit significant selective cytotoxic activity in respect of tumor cells and provide potentially useful chemotherapeutic agents for selective treatment of a range of cancers.
摘要翻译：本文公开了苯基中具有3'-取代基和4'-NR 5 R 6取代基的2-苯基吲唑化合物，其中R 5和R 6各自为氢或烷基，或者其中 4'-NR 5 R 6取代基是N-酰基（或N-苯甲酰基）。还公开了4'-N氨基磺酸盐形式的2-苯基唑化合物。这样的化合物在肿瘤细胞方面表现出显着的选择性细胞毒性活性，并且提供用于选择性治疗一系列癌症的潜在有用的化学治疗剂。

6. 发明申请

WO1995032297A1 NOVEL CHROMOSOME FRAGMENT AND ITS USE AS A VECTOR 审中-公开
标题翻译：新型色谱片，并作为矢量图使用
公开(公告)号：WO1995032297A1
公开(公告)日：1995-11-30
申请号：PCT/GB1995001195
申请日：1995-05-25
申请人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED , BROWN, William
发明人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED
IPC分类号： C12N15/85
CPC分类号： C12N15/90 , A01K2217/05 , A61K48/00
摘要： The present invention relates to the field of molecular genetics and in particular to artificial chromosomes, to their preparation in particular using telomere-directed chromosome fragmentation techniques and to their use as DNA vectors, for instance for application in gene therapy and animal transgenesis. Vectors of the invention comprise a chromosome fragment which fragment is at least partly responsible for centromere function of the parent chromosome and is capable of replication and segregation during cell cycle, and which is of such a size that it can be resolved using gel electrophoresis. Suitable fragments are derived from the human Y chromosome.
摘要翻译：本发明涉及分子遗传学领域，特别涉及人造染色体，特别是使用端粒指导的染色体断裂技术及其作为DNA载体的用途，例如用于基因治疗和动物转基因中的应用。本发明的载体包含染色体片段，其片段至少部分地负责母体染色体的着丝粒功能，并且能够在细胞周期期间复制和分离，并且其尺寸使得可以使用凝胶电泳解析。合适的片段衍生自人Y染色体。

7. 发明申请

WO1995015341A1 ANTIBODY AGAINST CARCINOEMBRYONIC ANTIGEN (CEA) 审中-公开
标题翻译：抗肿瘤抗原抗体（CEA）
公开(公告)号：WO1995015341A1
公开(公告)日：1995-06-08
申请号：PCT/GB1994002658
申请日：1994-12-05
申请人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED , CHESTER, Kerry, Anne , HAWKINS, Robert, Edward , BEGENT, Richard, Henry, John
发明人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED
IPC分类号： C07K16/30
CPC分类号： B82Y5/00 , A61K47/6853 , A61K47/6899 , A61K51/1048 , A61K2123/00 , C07K16/00 , C07K16/065 , C07K16/3007
摘要： The invention provides an antibody specific for carcinoembryonic antigen (CEA) which has a dissociation constant (Kd) of less than 5.0 nM for said antigen. The antibody is generally a single chain Fv (scFv) antibody. The antibody was initially obtained by screening a bacteriophage library for phage expressing high affinity CEA antibody. The antibody is useful for diagnosis and therapeutic treatment of colorectal tumours.
摘要翻译：本发明提供对于所述抗原具有小于5.0nM的解离常数（Kd）的癌胚抗原（CEA）特异性抗体。抗体通常是单链Fv（scFv）抗体。首先通过筛选表达高亲和力CEA抗体的噬菌体的噬菌体文库来获得抗体。该抗体可用于结肠直肠肿瘤的诊断和治疗。

8. 发明申请

WO1994025429A1 4-AMINO-FLUOROBENZAMIDES AND THEIR USE AS CYTOTOXIC PRODRUGS 审中-公开
标题翻译： 4-氨基荧光素及其作为细胞毒素的用途
公开(公告)号：WO1994025429A1
公开(公告)日：1994-11-10
申请号：PCT/GB1994000941
申请日：1994-05-03
申请人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED , SPRINGER, Caroline, Joy
发明人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED
IPC分类号： C07C237/36
CPC分类号： C07C237/36 , A61K47/6899 , B82Y5/00 , C07C229/60
摘要： The invention provides a compound which is a 3-fluorobenzamide of formula (A) wherein R-NH is the residue of an alpha -amino acid R-NH2 or oligopeptide R-NH2, and M is a nitrogen mustard group of formula (A') wherein Y and L, which may be the same or differnt in a molecule, are leaving groups; or a pharmaceutically acceptable salt thereof. The compounds are useful as prodrugs for treating cancer.
摘要翻译：本发明提供了一种化合物，其是式（A）的3-氟苯甲酰胺，其中R-NH是α-氨基酸R-NH 2或寡肽R-NH 2的残基，M是式（A' ）其中在分子中可以相同或不同的Y和L是离去基团; 或其药学上可接受的盐。该化合物可用作治疗癌症的前药。

9. 发明申请

WO1994023753A1 CANCER THERAPY, USING ANTIBODY CONJUGATES, IN COMBINATION WITH A VASOACTIVE AGENT 审中-公开
标题翻译：癌症治疗，使用抗体联合药物与血液代用品联合使用
公开(公告)号：WO1994023753A1
公开(公告)日：1994-10-27
申请号：PCT/GB1994000831
申请日：1994-04-20
申请人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED , PEDLEY, Rosamund, Barbara , BEGENT, Richard, Henry, John
发明人： CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED
IPC分类号： A61K47/48
CPC分类号： A61K31/35 , A61K47/6899 , B82Y5/00
摘要： The invention provides a two component system for the treatment of cancer comprising: (i) a tumour-directed antibody linked to a toxic agent or linked to an enzyme capable of converting a prodrug to a toxic agent; and (ii) an agent having the ability to restrict blood flow at the site of a tumour. Preferably the agent is a flavonoid derivative such as 5,6 dimethylxanthenone acetic acid or flavone acetic acid.
摘要翻译：本发明提供了用于治疗癌症的双组分系统，其包括：（i）与毒性剂连接或连接到能够将前药转化为毒性剂的酶连接的肿瘤引导的抗体; 和（ii）具有限制在肿瘤部位的血液流动的能力的药剂。优选地，试剂是类黄酮衍生物，例如5,6二甲基呫吨酮乙酸或黄酮乙酸。

10. 发明申请

WO1994003585A1 A METHOD FOR MAINTAINING EMBRYONIC STEM CELLS AND AVIAN FACTOR USEFUL FOR SAME 审中-公开
标题翻译：维持胚胎干细胞的方法和用于其的AVI因子
公开(公告)号：WO1994003585A1
公开(公告)日：1994-02-17
申请号：PCT/AU1993000399
申请日：1993-08-04
申请人： COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ... , CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED , DYER, Shanny, Leigh , JENNINGS, Philip , LOCKETT, Trevor , HEATH, John, Kaye
发明人： COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ... , CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED
IPC分类号： C12N05/06
CPC分类号： C12N15/8775 , C07K14/52 , C12N5/0606 , C12N2502/13
摘要： The present invention provides a method for maintaining in vitro animal embryonic stem (ES) cells without substantial differentiation, said method comprising culturing said ES cells in the presence of a feeder layer comprising chicken embryonic fibroblasts. The present invention also contemplates an isolated chick-derived ES stem cell factor obtainable from chicken embryonic fibroblasts and chick embryonic extracts.
摘要翻译：本发明提供了一种维持体外动物胚胎干细胞（ES）细胞而不显着分化的方法，所述方法包括在包含鸡胚胎成纤维细胞的饲养层存在下培养所述ES细胞。本发明还考虑了可从鸡胚胎成纤维细胞和雏鸡胚胎提取物获得的分离的来自雏鸡的ES干细胞因子。

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