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1. 发明申请

WO2012016929A1 MASS SPECTROMETRIC DIAGNOSIS OF SEPSIS WITHOUT BLOOD CULTURE 审中-公开
标题翻译：没有血液文化的食物的大量光谱诊断
公开(公告)号：WO2012016929A1
公开(公告)日：2012-02-09
申请号：PCT/EP2011/063120
申请日：2011-07-29
申请人： BRUKER DALTONIK GMBH , KOSTRZEWA, Markus , PUSCH, Wolfgang , MAIER, Thomas , MICHELMANN, Karsten , FRANZEN, Jochen
发明人： KOSTRZEWA, Markus , PUSCH, Wolfgang , MAIER, Thomas , MICHELMANN, Karsten , FRANZEN, Jochen
IPC分类号： C12Q1/04 , G01N33/68 , C12M1/34 , C12M3/00
CPC分类号： C12Q1/04 , G01N21/77 , G01N33/6851 , G01N2021/7786 , G01N2800/26
摘要： The invention relates to methods and instruments for the rapid detection and rapid mass spectrometric identification of microbial infective agents in blood or other body fluids. The invention recognizes that blood is not a good environment for the cultivation of microbes and provides a method which (a) largely destroys or dissolves the human particles in body fluids, such as erythrocytes and leukocytes in blood, without impairing the ability of the microbes to reproduce, (b) separates the microbial pathogens from the fluid, (c) cultivates them in a nutrient broth which contains none of the antimicrobial components of the body fluids, (d) separates them from the nutrient broth, and (e) identifies the microbes by a mass spectrum of the microbial proteins. The dissolution of the human particles also releases the microbes nesting in macrophages. The cultivation in an optically clear nutrient broth with optimum composition not only accelerates the propagation of the microbes compared to all other cultivation methods, but also makes it possible to continuously measure their quantitative growth starting from a low microbe density. This firstly allows the mass spectrometric identification to be carried out at the earliest possible time, secondly provides a positive detection of microbes far ahead of their identification, which can be lifesaving for the patient; and thirdly makes it possible to start the determination of resistances early.
摘要翻译：本发明涉及快速检测和快速质谱鉴定血液或其他体液中微生物感染因子的方法和仪器。本发明认识到，血液不是微生物培养的良好环境，并且提供了（a）大大地破坏或溶解体液中的人体颗粒如血液中的红细胞和白细胞的方法，而不会损害微生物的能力（b）将微生物病原体从流体中分离出来，（c）在不含体液的抗微生物成分的营养液中培养它们，（d）将其与营养肉汤分开，以及（e）微生物通过微生物蛋白的质谱。人类颗粒的溶解也释放出嵌入巨噬细胞的微生物。在具有最佳组成的光学透明营养肉汤中培养不仅加速微生物与所有其他培养方法相比的繁殖，而且可以从低微生物密度开始连续测量其定量生长。这首先允许在最早的时候进行质谱鉴定，其次提供远远超出其鉴定的微生物的阳性检测，这可以为患者提供救生。第三，可以提前开始确定电阻。

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