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1. 发明申请

WO2012080844A1 CELL CULTURE MEDIUM 审中-公开
标题翻译：细胞培养液
公开(公告)号：WO2012080844A1
公开(公告)日：2012-06-21
申请号：PCT/IB2011/003265
申请日：2011-12-16
申请人： BIOLAMINA AB , TRYGGVASON, Karl , RODIN, Sergey
发明人： TRYGGVASON, Karl , RODIN, Sergey
IPC分类号： C07K14/78 , C12N5/00
CPC分类号： C07K14/78 , C12N5/0606 , C12N5/0696 , C12N2501/602 , C12N2501/603 , C12N2501/605 , C12N2501/608 , C12N2510/00 , C12N2533/52 , Y10T428/13
摘要： The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11 ), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11 ) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein.
摘要翻译：本公开涉及分离的层粘连蛋白-521，制备重组层粘连蛋白521的方法，表达重组层粘连蛋白-521的宿主细胞和含有层粘连蛋白-51的组合物。层粘连蛋白-521可以维持干细胞的体外多能性，实现自我更新，并能使人类胚胎干细胞的单细胞存活。当多能人胚胎干细胞在用重组层粘连蛋白-51（层粘连蛋白-11）包被的平板上培养时，在不存在分化抑制剂或饲养细胞的情况下，胚胎干细胞增殖并保持其多能性。还已经发现人重组层粘连蛋白-51（层粘连蛋白-11）在完全解离成单细胞悬液后提供干细胞的单细胞存活。含有至多3.9ng / ml的β成纤维细胞生长因子（bFGF）的有用的细胞培养基也在本文中描述。

2. 发明申请

WO2011110886A1 COMPOSITION AND METHOD FOR ENABLING PROLIFERATION OF PLURIPOTENT HUMAN STEM CELLS 审中-公开
标题翻译：促进多能干细胞增殖的组合物和方法
公开(公告)号：WO2011110886A1
公开(公告)日：2011-09-15
申请号：PCT/IB2010/000823
申请日：2010-03-09
申请人： BIOLAMINA AB , TRYGGVASON, Karl , DOMOGATSKAYA, Anna , RODIN, Sergey
发明人： TRYGGVASON, Karl , DOMOGATSKAYA, Anna , RODIN, Sergey
IPC分类号： C12N5/0735
CPC分类号： C12N5/0606 , C12N2500/90 , C12N2500/98 , C12N2533/52
摘要： Compositions and processes for culturing human stem cells in vitro in an undifferentiated state are disclosed. In this regard, human embryonic stem cells proliferated and maintained their pluripotency when cultured on plates coated with recombinant laminin-10 (laminin-511 ).
摘要翻译：公开了以未分化状态体外培养人类干细胞的组合物和方法。就此而言，当在用重组层粘连蛋白-10（层粘连蛋白-511）包被的平板上培养时，人胚胎干细胞增殖并维持其多能性。

3. 发明申请

WO2012117297A1 ISOLATED LAMININ-421 审中-公开
标题翻译：隔离的LAMININ-421
公开(公告)号：WO2012117297A1
公开(公告)日：2012-09-07
申请号：PCT/IB2012/000519
申请日：2012-03-01
申请人： BIOLAMINA AB , TRYGGVASON, Karl , RODIN, Sergey
发明人： TRYGGVASON, Karl , RODIN, Sergey
IPC分类号： C12N15/12 , C07K14/78 , A61K38/39
CPC分类号： C07K14/78 , A61K38/00 , C07K2319/033 , C07K2319/036 , C07K2319/09 , C07K2319/41 , C07K2319/43
摘要： The present disclosure provides isolated laminin-421, methods for making recombinant laminin-421, and host cells that express recombinant laminin-421. The present disclosure also provides nucleic acid sequences encoding full length human laminin β2 chain, expression vectors and host cells thereof.
摘要翻译：本公开提供分离的层粘连蛋白421，用于制备重组层粘连蛋白421的方法和表达重组层粘连蛋白421的宿主细胞。本公开还提供了编码全长人层粘连蛋白β2链，表达载体和宿主细胞的核酸序列。

4. 发明申请

WO2012080842A1 RECOMBINANT LAMININ-521 审中-公开
标题翻译：重组LAMININ-521
公开(公告)号：WO2012080842A1
公开(公告)日：2012-06-21
申请号：PCT/IB2011/003258
申请日：2011-12-16
申请人： BIOLAMINA AB , TRYGGVASON, Karl , RODIN, Sergey
发明人： TRYGGVASON, Karl , RODIN, Sergey
IPC分类号： C07K14/78 , C12N5/00 , A61K38/39
CPC分类号： C07K14/78 , C12N5/0606 , C12N5/0696 , C12N2501/602 , C12N2501/603 , C12N2501/605 , C12N2501/608 , C12N2510/00 , C12N2533/52 , Y10T428/13
摘要： The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with a matrix of recombinant laminin-521 (laminin 11), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein.
摘要翻译：本公开涉及分离的层粘连蛋白-521，制备重组层粘连蛋白521的方法，表达重组层粘连蛋白-521的宿主细胞和含有层粘连蛋白-51的组合物。层粘连蛋白-521可以维持干细胞的体外多能性，实现自我更新，并能使人类胚胎干细胞的单细胞存活。当多能人胚胎干细胞在涂有重组层粘连蛋白-51（层粘连蛋白11）基质的平板上培养时，在不存在分化抑制剂或饲养细胞的情况下，胚胎干细胞增殖并保持其多能性。还已经发现人重组层粘连蛋白-51（层粘连蛋白-11）在完全解离成单细胞悬液后提供干细胞的单细胞存活。含有至多3.9ng / ml的β成纤维细胞生长因子（bFGF）的有用的细胞培养基也在本文中描述。

5. 发明申请

WO2013041961A1 CELL CULTURE SUBSTRATE COMPRISING A LAMININ AND A CADHERIN 审中-公开
标题翻译：细胞培养基底层包含一个层压蛋白和一个CADHERIN
公开(公告)号：WO2013041961A1
公开(公告)日：2013-03-28
申请号：PCT/IB2012/002214
申请日：2012-09-19
申请人： TRYGGVASON, Karl , RODIN, Sergey
发明人： TRYGGVASON, Karl , RODIN, Sergey
IPC分类号： C07K14/78 , C07K14/705 , C12N5/00 , C12M3/00
CPC分类号： C12N5/0607 , C07K14/705 , C07K14/78 , C12M25/00 , C12N5/0068 , C12N5/0606 , C12N5/0696 , C12N2501/115 , C12N2533/52 , C12N2533/90
摘要： The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11 ), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11 ) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein.
摘要翻译：本公开涉及分离的层粘连蛋白-521，制备重组层粘连蛋白521的方法，表达重组层粘连蛋白-521的宿主细胞和含有层粘连蛋白-51的组合物。层粘连蛋白-521可以维持干细胞的体外多能性，实现自我更新，并能使人类胚胎干细胞的单细胞存活。当多能人胚胎干细胞在用重组层粘连蛋白-51（层粘连蛋白-11）包被的平板上培养时，在不存在分化抑制剂或饲养细胞的情况下，胚胎干细胞增殖并保持其多能性。还已经发现人重组层粘连蛋白-51（层粘连蛋白-11）在完全解离成单细胞悬液后提供干细胞的单细胞存活。含有至多3.9ng / ml的β成纤维细胞生长因子（bFGF）的有用的细胞培养基也在本文中描述。

6. 发明申请

WO2011024070A2 COMPOSITION AND METHOD FOR PROLIFERATING UNDIFFERENTIATED STEM CELLS 审中-公开
标题翻译：增殖未分化干细胞的组成和方法
公开(公告)号：WO2011024070A2
公开(公告)日：2011-03-03
申请号：PCT/IB2010/002251
申请日：2010-08-24
申请人： DOMOGATSKAYA, Anna , RODIN, Sergey , TRYGGVASON, Karl
发明人： DOMOGATSKAYA, Anna , RODIN, Sergey , TRYGGVASON, Karl
IPC分类号： C12N5/00
CPC分类号： C12N5/0606 , C12N2533/52
摘要： The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, that both maintain stem cells in vitro pluripotency and enable self-renewal. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511 ) or their functional domains, in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferated and maintained their pluripotency.
摘要翻译：本公开涉及开发诸如细胞外基质的组合物，以及使用它们的方法，其既保持干细胞的体外多能性又能够自我更新。就此而言，已经发现当在涂有重组层粘连蛋白-10（层粘连蛋白-511）或其功能结构域的平板上培养多能小鼠和人类胚胎干细胞时，在不存在分化抑制剂或饲养细胞的情况下，胚胎干细胞增殖并维持它们的多能性。

7. 发明申请

WO2011114237A2 COMPOSITION AND METHOD FOR ENABLING PROLIFERATION OF PLURIPOTENT HUMAN STEM CELLS 审中-公开
标题翻译：用于促进人类干细胞增殖的组合物和方法
公开(公告)号：WO2011114237A2
公开(公告)日：2011-09-22
申请号：PCT/IB2011/000982
申请日：2011-03-17
申请人： TRYGGVASON, Karl , RODIN, Sergey , DOMOGATSKAYA, Anna
发明人： TRYGGVASON, Karl , RODIN, Sergey , DOMOGATSKAYA, Anna
IPC分类号： C12N5/00
CPC分类号： C12N5/0606 , C12N2500/25 , C12N2500/90 , C12N2500/98 , C12N2501/115 , C12N2501/998 , C12N2533/52
摘要： Compositions and processes for culturing human stem cells in vitro in an undifferentiated state are disclosed. In this regard, human embryonic stem cells proliferated and maintained their pluripotency when cultured on plates coated with recombinant laminin-10 (laminin-511 ).
摘要翻译：公开了以未分化状态体外培养人类干细胞的组合物和方法。在这方面，人胚胎干细胞在用重组层粘连蛋白-10（层粘连蛋白-511）包被的平板上培养时增殖并维持其多能性。

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