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    • 2. 发明申请
    • ELISA SIGNAL AMPLIFICATION SYSTEM USING MAGNETIC BEAD MOVEMENT DETECTION
    • 使用磁珠运动检测的ELISA信号放大系统
    • WO2011045570A3
    • 2011-10-27
    • PCT/GB2010001913
    • 2010-10-14
    • CENTRE NAT RECH SCIENTUNIV PORTSMOUTHECOLE NORMALE SUPERIEURE LNANOTECNOLOGIASBENSIMON DAVIDDE FREITAS PAULO JORGE PEIXEIROFIRMAN KEITH
    • BENSIMON DAVIDDE FREITAS PAULO JORGE PEIXEIROFIRMAN KEITH
    • G01N33/53C12Q1/25G01N33/58
    • G01N33/54306G01N33/54326G01N33/54366G01N33/581
    • The invention relates to a detection system for detecting a signal in a sandwich ELISA assay, the detection system comprising an amplification system and a sensing apparatus, the amplification system comprising: a binding molecule releasably attached to a first releasing molecule; and a plurality of manipulating agents immobilised on a substrate, and wherein the sensing apparatus comprises: a substrate; an elongate molecule attached at one end to the substrate; a magnetic bead attached to the other end of the elongate molecule; an element to produce a magnetic field; and an electronic sensor to detect movement of the magnetic bead, wherein the element produces a magnetic field which exerts a force on the magnetic bead to stretch the elongate molecule into an extended form, and wherein the sensor detects movement of the magnetic bead resulting from movement of the elongate molecule, wherein, in use, the first releasing molecule is released from the binding molecule by a cleaving agent, wherein, once released, the first releasing molecule causes a plurality of manipulating agents to be released from the substrate, wherein, once released, the manipulating agents interact with the elongate molecule in the sensing apparatus and cause movement of the elongate molecule, and wherein the movement of the elongate molecule causes movement of the magnetic bead which is detected by the electronic sensor.
    • 本发明涉及一种用于在夹心ELISA测定中检测信号的检测系统,所述检测系统包括扩增系统和感测装置,所述扩增系统包括:可释放地连接到第一释放分子的结合分子; 以及固定在基板上的多个操纵剂,其中所述感测装置包括:基板; 一个细长分子在一端附着到基底上; 连接到细长分子的另一端的磁珠; 产生磁场的元素; 以及用于检测磁珠的运动的电子传感器,其中所述元件产生磁场,所述磁场在所述磁珠上施加力以将所述细长分子拉伸成延伸形式,并且其中所述传感器检测由运动产生的磁珠的移动 其中,在使用中,所述第一释放分子通过裂解剂从所述结合分子释放,其中一旦释放,所述第一释放分子使多个操作剂从所述底物释放,其中,一次 所述操纵剂与所述感测装置中的细长分子相互作用并引起所述细长分子的移动,并且其中所述细长分子的移动引起由所述电子传感器检测到的所述磁珠的移动。