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1. 发明申请

WO1995012813A1 METHOD TO DISTINGUISH HEMATOPOIETIC PROGENITOR CELLS 审中-公开
标题翻译：鉴别HEMATOPOIETIC PROGENITOR细胞的方法
公开(公告)号：WO1995012813A1
公开(公告)日：1995-05-11
申请号：PCT/US1994012657
申请日：1994-11-03
申请人： BECTON, DICKINSON AND COMPANY
发明人： BECTON, DICKINSON AND COMPANY , OLWEUS, Johanna , LUND-JOHANSEN, Fridtjof , TERSTAPPEN, Leon, W.
IPC分类号： G01N33/49
CPC分类号： C12N5/0647 , G01N33/56977
摘要： The expression of various cell adhesion molecules and growth factor receptors was examined on human progenitor cells (i.e., CD34 /CD38 ). Certain changes in the expression of one or more of these molecules and receptors correlates with the progression of cell from non-lineage committed to commitment to a specific lineage. Using one or more markers for these molecules and/or receptors in combination with markers for CD34 and CD38 will enable one to identify and isolate each of the different progenitor cell populations.
摘要翻译：在人祖细胞（即CD34 + / CD38 +）上检查各种细胞粘附分子和生长因子受体的表达。这些分子和受体中的一个或多个的表达的某些变化与从承诺到特定谱系的非谱系的细胞的进展相关。将这些分子和/或受体结合用于CD34和CD38的标记物使用一种或多种标记物将使得能够鉴别和分离每种不同的祖细胞群体。

2. 发明申请

WO1998014783A1 GRANULOCYTIC AND MONOCYTIC DIFFERENTIATION OF CD34hi CELLS ASSOCIATED WITH DISTINCT CHANGES IN THE EXPRESSION OF THE PU.1 REGULATED MOLECULES, CD64 AND M-CSFR 审中-公开
标题翻译：与表达PU.1调节分子，CD64和M-CSFR相关的不同变化相关的CD34细胞的颗粒细胞和单核细胞差异
公开(公告)号：WO1998014783A1
公开(公告)日：1998-04-09
申请号：PCT/US1997017453
申请日：1997-09-29
申请人： BECTON DICKINSON AND COMPANY
发明人： BECTON DICKINSON AND COMPANY , OLWEUS, Johanna , LUND-JOHANSEN, Fridtjof , THOMPSON, Peter
IPC分类号： G01N33/53
CPC分类号： C12N5/0645 , C12N5/0642 , C12N2501/125 , C12N2501/14 , C12N2501/22 , C12N2501/23 , G01N33/56972
摘要： The present invention demonstrates that M-CSF responsiveness and the M-CSFR expression can be used to discriminate monocytic and granulocytic cells within a population of cells which strongly expresses the CD34 antigen (CD34 ). Briefly, the method comprises isolating phenotypically and functionally defined CD34 subsets, and staining with anti-M-CSFR monoclonal antibodies to measure expression on these primitive progenitors and cells committed to the granulocytic and monocytic lineages, based upon expression of M-CSFR. CD34 M-CSFR cells are highly clonogenic and approximately 70 % of the colonies are CFU-M (monocytic), whereas less than 20 % were CFU-G (granulocytic). In contrast, CD34 cells that were positive for the granulo-monocytic marker CD64 and negative for the M-CSFR contained high frequencies of 91 % pure CFU-Gs. After 60h in culture, CD34 M-CSFR cells developed into distinct populations of M-CSFR and M-CSFR cells. These two populations gave rise almost exclusively to monocytes and granulocytes, respectively. This result demonstrates that M-CSF target specificity among human hematopoietic progenitor cells is determined by lineage-specific regulation of the M-CSFR and demonstrate that M-CSFR is a useful marker to discriminate CFU-Ms from CFU-Gs.
摘要翻译：本发明证实M-CSF响应性和M-CSFR表达可用于区分强烈表达CD34抗原（CD34ζ）的细胞群体内的单核细胞和粒细胞。简而言之，该方法包括分离表型和功能定义的CD34 +亚型，并用抗M-CSFR单克隆抗体染色，以测量基于M-CSFR表达的这些原始祖细胞和致粒细胞和单核细胞谱系的细胞的表达。 CD34细胞是高度克隆的，大约70％的菌落是CFU-M（单核细胞），而小于20％是CFU-G（粒细胞）。相比之下，对于粒细胞单核细胞标志物CD64呈阳性且M-CSFR阴性的CD34细胞含有91％纯CFU-Gs的高频率。培养60小时后，将CD34M-CSFR细胞发育成M-CSFR和M-CSFR细胞的不同群体。这两个群体几乎分别产生单核细胞和粒细胞。该结果表明，通过M-CSFR的谱系特异性调节来确定人造血祖细胞中的M-CSF靶特异性，并证明M-CSFR是鉴别CFU-Ms与CFU-Gs的有用标记。

3. 发明申请

WO1998014561A1 IDENTIFICATION OF A CD34+ BONE MARROW PRECURSOR FOR DENDRITIC CELLS IN BLOOD AND LYMPHOID TISSUES 审中-公开
标题翻译：鉴定血液和淋巴组织中CD34 +骨髓前体细胞
公开(公告)号：WO1998014561A1
公开(公告)日：1998-04-09
申请号：PCT/US1997017454
申请日：1997-09-29
申请人： BECTON DICKINSON AND COMPANY
发明人： BECTON DICKINSON AND COMPANY , OLWEUS, Johanna , LUND-JOHANSEN, Fridtjof
IPC分类号： C12N05/06
CPC分类号： G01N33/56972 , C07K16/2866 , C12N5/0639 , C12N2501/22 , C12N2501/25 , G01N33/56966 , Y10S436/824
摘要： Dendritic cells (DCs) are the primary antigen presenting cells during the initiation of T cell-dependent immune responses. The cells originate from the bone marrow and have been suggested to represent a distinct cell lineage. However, distinct DC precursors have not been identified in bone marrow, and mature monocytes can also give rise to DCs. The instant invention presents a distinct DC precursor among bone marrow CD34+ cells. The cells express high levels of the interleukin-3 receptor alpha chain and CD4 and can be uniquely identified also in blood and lymphoid tissues by this phenotype.
摘要翻译：树突状细胞（DCs）是T细胞依赖性免疫应答开始期间的主要抗原呈递细胞。细胞起源于骨髓，并被认为是代表不同的细胞谱系。然而，在骨髓中尚未发现不同的DC前体，而成熟的单核细胞也可能引起DCs。本发明在骨髓CD34 +细胞中呈现明显的DC前体。细胞表达高水平的白细胞介素-3受体α链和CD4，并且可以通过该表型在血液和淋巴组织中独特地鉴定。

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