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2. 发明申请

WO2002068454A2 PROTEINS IN A POROUS SUPPORT 审中-公开
标题翻译：蛋白质多孔支持
公开(公告)号：WO2002068454A2
公开(公告)日：2002-09-06
申请号：PCT/US2002/005755
申请日：2002-02-21
申请人： BATELLE MEMORIAL INSTITUTE
发明人： ACKERMAN, Eric, J. , LIU, jun , CHENGHONG, Lei
IPC分类号： C07K1/00
CPC分类号： C12N11/14 , C12N9/16
摘要： A protein system is described in which a protein is bound within a matrix material that has pores that are sized to achieve excellent properties such as: activity, protein density, and stability. In a preferred embodiment, the pore sizes range from 50 to 400 A. One protein that has demonstrated surprisingly good results in this system is OPH. This protein is known to degrade organophosphorus compounds such as are found in chemical weapons and pesticides. Novel methods of forming the protein system and methods of making OPH are also described. Also described is a protein system including a porous matrix material and a protein disposed within the porous matrix material; wherein the protein system contains at least 0.01 mmol of protein per gram of matrix material and exhibits an activity at least 2 times greater that the activity of a protein system that has been formed under identical conditions on a normal silica matrix material. Methods of making protein systems with noncovalent bonding to entrap proteins in the porous matrix are also described.
摘要翻译：描述了一种蛋白质系统，其中蛋白质结合在具有孔的基质材料中，孔的尺寸被设计成实现优异的性质，例如：活性，蛋白质密度和稳定性。在优选的实施方案中，孔径范围为50至400A。在该体系中已经显示出令人惊奇的良好结果的一种蛋白质是OPH。已知这种蛋白质会降解有机磷化合物，如在化学武器和农药中发现的。还描述了形成蛋白质系统的新方法和制备OPH的方法。还描述了包括多孔基质材料和设置在多孔基质材料内的蛋白质的蛋白质系统; 其中所述蛋白质系统每克基质材料含有至少0.01毫摩尔的蛋白质，并且表现出在相同条件下在正常二氧化硅基质材料上形成的蛋白质体系的活性的至少2倍以上的活性。还描述了制备具有非共价键合的蛋白质系统以捕获多孔基质中的蛋白质的方法。

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