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1. 发明申请

WO2004076617A3 METHOD FOR THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS 审中-公开
标题翻译：生产多种不饱和脂肪酸的方法
公开(公告)号：WO2004076617A3
公开(公告)日：2004-10-28
申请号：PCT/EP2004000771
申请日：2004-01-29
申请人： BASF PLANT SCIENCE GMBH , RENZ ANDREAS , HEINZ ERNST , ABBADI AMINE , DOMERGUE FREDERIC , ZANK THORSTEN
发明人： RENZ ANDREAS , HEINZ ERNST , ABBADI AMINE , DOMERGUE FREDERIC , ZANK THORSTEN
IPC分类号： C12N9/10 , C12N15/82 , C12P7/64
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The invention relates to a method for producing polyunsaturated fatty acids in an organism by introducing nucleic acids coding for polypeptides with acyl-CoA:lysophospholipid-acyl transferase activity into the organism. Advantageously, said nucleic acid sequences can optionally be expressed in the transgenic organism along with additional nucleic acid sequences coding for polypeptides of fatty acid metabolism or lipid metabolism biosynthesis. The invention also relates to the nucleic acid sequences, nucleic acid structures containing said nucleic acid sequences, vectors containing the inventive nucleic acid sequences, and/or the nucleic acid structures and transgenic organisms containing said nucleic acid sequences, nucleic acid structures, and/or vectors. The invention further relates to oils, lipids, and/or fatty acids that are produced according the inventive method, and the use thereof.
摘要翻译：本发明涉及一种用于生产在由核酸的生物体的多不饱和脂肪酸的方法被引入到生物体中，其用于与酰基辅酶A的多肽代码：溶血磷脂酰基转移酶酶活性的编码。有利地，这些核酸序列可以任选地与编码脂肪酸或脂质代谢的生物合成的多肽的其他核酸序列一起在转基因生物体中表达。本发明还涉及核酸序列，含有根据本发明的核酸序列的核酸构建体，包含核酸序列的载体和/或含有上述核酸序列的核酸构建体和转基因生物，核酸构建体和/或载体。本发明的另一部分涉及通过erfindungsgemäbetaen方法制备的油，脂质和/或脂肪酸及其用途。

2. 发明申请

WO2005012316A3 METHOD FOR THE PRODUCTION OF MULTIPLY-UNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：用于生产不饱和脂肪酸的转基因多
公开(公告)号：WO2005012316A3
公开(公告)日：2005-10-06
申请号：PCT/EP2004007957
申请日：2004-07-16
申请人： BASF PLANT SCIENCE GMBH , ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
发明人： ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
IPC分类号： A23D9/00 , C11B1/02 , C12N9/02 , C12N9/10 , C12N15/82 , C12P7/64 , A01K67/027
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Delta-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Delta-6 desaturase, a Delta-5 desaturase, Delta-4 desaturase and/or Delta-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated omega-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Delta-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of omega-3 fatty acids, oils or lipids with omega-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with omega-3 desaturases, for example a omega-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a omega-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceae such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.
摘要翻译：本发明涉及一种用于通过核酸的生物体制备的多个unge饱和脂肪酸的方法被引入到生物体中，这对于多肽与Δ-5-延伸酶活性的代码。有利地，这些核酸序列可以在生物体中，任选地与编码所述脂肪酸或脂质的生物合成的多肽的另外的核酸序列表达。特别有利的是编码Δ-4-Desaturaseund /或Δ-6去饱和酶，Δ-5去饱和酶的Δ-6延长酶的活性的核酸序列。有利的是，这些去饱和酶和延伸酶海链藻，裸藻或Ostreococcus。此外，本发明涉及用于生产油和/或三酰基甘油酯与长链多不饱和脂肪酸的升高的内容的处理。此外，在一个优选的实施方案中，本发明涉及用于生产不饱和ω-3脂肪酸和用于生产具有的不饱和脂肪酸含量增加的甘油三酯的方法的过程中，具有超过三个双键尤其ω-3脂肪酸。本发明涉及产生转基因生物，优选的转基因植物或转基因微生物，其具有具有由于延伸酶的表达ω-3双键的不饱和的ω-3脂肪酸，油或脂质的含量增加，并在本发明中连接有利的过程中去饱和酶使用与ω-3去饱和酶，例如，从家庭Pythiaceae的真菌，如属疫ω-3去饱和酶，例如属和种致病疫霉或从藻类ω-3去饱和酶诸如家庭绿枝藻纲例如的属Ostreococcus具体的属和种Ostreococcus桃源或硅藻如海链藻属特别是属和种海链pseudonana。本发明还涉及含有本发明核酸序列的核酸序列，核酸构建体，载体和有机体，将含有包含上述核酸序列，核酸构建体和/或载体的核酸序列和/或核酸构建体，和转基因生物体的载体。本发明的另一部分涉及通过本发明和它们的使用过程中产生的油，脂质和/或脂肪酸。本发明还涉及到的不饱和脂肪酸和甘油三酯具有不饱和脂肪酸的含量增加及其用途。

3. 发明申请

WO02057465A3 METHOD FOR PRODUCING POLYUNSATURATED FATTY ACIDS, NOVEL BIOSYNTHESIS GENES AND NOVEL PLANT EXPRESSION CONSTRUCTS 审中-公开
标题翻译：用于生产多不饱和脂肪酸，合成新的和新工厂的表达构建
公开(公告)号：WO02057465A3
公开(公告)日：2003-06-12
申请号：PCT/EP0200462
申请日：2002-01-18
申请人： BASF PLANT SCIENCE GMBH , LERCHL JENS , RENZ ANDREAS , HEINZ ERNST , DOMERGUE FREDERIC , ZAEHRINGER ULRICH
发明人： LERCHL JENS , RENZ ANDREAS , HEINZ ERNST , DOMERGUE FREDERIC , ZAEHRINGER ULRICH
IPC分类号： A01H5/00 , A01K67/027 , A23D9/007 , A23L1/30 , A61K8/30 , A61K8/34 , A61K8/37 , A61K31/201 , A61K31/202 , A61K31/231 , A61K31/232 , A61K31/7088 , A61K36/00 , A61K39/395 , A61K45/00 , A61P3/06 , C07K16/40 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/02 , C12N15/09 , C12N15/53 , C12N15/82 , C12P7/64 , C12Q1/02 , C12Q1/26 , G01N33/15 , G01N33/50 , C11B1/00 , C12N15/11 , C12P7/62 , C12Q1/68 , G01N33/573
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The invention relates to a method for producing unsaturated fatty acids comprising at least two double bonds, and a method for producing triglycerides having an increased content of polyunsaturated fatty acids comprising at least two double bonds. The invention also relates to the advantageous use of the nucleic acid sequences of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 9, SEQ ID NO: 11 or SEQ ID NO: 18 in the inventive methods, and for producing a transgenic organism, preferably a transgenic plant or a transgenic micro-organism having an increased content of fatty acids, oils or lipids containing unsaturated C18, C20, or C22 fatty acids. The invention further relates to novel desaturases comprising the sequences of SEQ ID NO. 1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 11, or the homologues, derivatives or analogues thereof, and gene constructs comprising said genes or the homologues, derivatives or analogues thereof, in addition to the use of the same, either alone or combined with biosynthesis genes of polyunsaturated fatty acids, as advantageously represented in SEQ ID NO: 7 and SEQ ID NO: 9. Furthermore, the invention relates to isolated nucleic acid sequences, expression cassettes containing said nucleic acid sequences, vectors, and transgenic organisms containing at least one nucleic acid sequence or an expression cassette. The invention also relates to unsaturated fatty acids comprising at least two double bonds and triglycerides having an increased content of unsaturated fatty acids comprising at least two double bonds, and the use of the same. Finally, the invention relates to multi-expression cassettes for seed-specific expression, in addition to vectors or organisms comprising a desaturase gene, either alone or combined with other desaturases having the sequence of SEQ ID NO: 7 and/or elongase genes having the sequence of ID NO: 9 or the homologues, derivatives or analogues of the same, expressed by means of the above-mentioned expression cassettes.
摘要翻译：本发明涉及一种用于制备具有至少两个双键和/或用于生产甘油三酯的具有多不饱和脂肪酸与多不饱和脂肪酸具有至少两个双键的至少höhtem含量增加的内容的方法的不饱和脂肪酸的方法。本发明还涉及有利地使用的核酸序列的SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5，SEQ ID NO：9，SEQ ID NO：11或SEQ ID NO：18中的过程和用于生产的转基因生物体，优选转基因植物或具有脂肪酸，油或脂类具有不饱和C18，C20，C22或脂肪酸含量增加的转基因微生物。本发明还涉及新的去饱和酶与序列SEQ ID号 1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：11或它的同系物，衍生物或它们的类似物，和包含所述基因的基因构建体或他们的同系物，衍生物或类似物，并单独或与生物合成基因组合使用它们polyungesätigter 脂肪酸，例如有利地在SEQ ID NO：9：7和SEQ ID NO。本发明还涉及分离的核酸序列; 表达盒包含Nukleinesäuresequenzen。载体，以及含有至少一种核酸序列或表达盒的转基因生物。本发明还涉及一种具有至少两个双键和具有具有至少两个双键和其用途的不饱和脂肪酸含量增加的甘油三酯不饱和脂肪酸。本发明还涉及用于种子特异性表达，其包括单独或与具有SEQ ID NO：1的序列的其它去饱和酶组合的去饱和酶基因的多表达盒和载体或生物体：7和/或延长酶基因与去SEQ ID NO：9或它的同系物，衍生物或类似物包括使用vesagter的表达盒。

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