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    • 6. 发明申请
    • METHOD FOR PRODUCING RECOMBINANT ORGANISMS
    • 生产重组有机体的方法
    • WO2004085644A2
    • 2004-10-07
    • PCT/EP2004002881
    • 2004-03-19
    • BASF PLANT SCIENCE GMBHBADUR RALFREISS BERND
    • BADUR RALFREISS BERND
    • C07K14/415C12N15/10C12N15/82C12N15/11
    • C07K14/415C12N15/102C12N15/8213
    • The present invention relates to methods for producing a recom­binant eukaryotic organisms, preferably a recombinant plant, comprising the step of decreasing the chromatin assembly capacity of said organism. The method may be used for introducing sequences into the chromosomal DNA of a eukaryotic cell either by illegitimate insertion or, preferably, by homologous recombina­tion. The method results in increased transformation efficacy and/or increased frequency of homologous recombination. The invention furthermore relates to expression constructs suitable for expressing at least part of a nucleic acid sequence encoding for a chromatin assembly factor protein (e.g., in form of antisense or double-stranded RNA) suitable for decreasing expression or functionality of at least one chromatin assembly factor protein in plant or plant cells. Object of the invention are furthermore recombinant plant cells or plants comprising a decreased chromatin assembly capacity, preferably a decreased expression or functionality at least one chromatin assembly factor and the use of said recombinant plants for the production of food, feeds, seed, pharmaceuticals or fine chemicals.
    • 本发明涉及用于生产重组真核生物(优选重组植物)的方法,其包括降低所述生物体的染色质组装能力的步骤。 该方法可用于通过非法插入或优选通过同源重组将序列引入真核细胞的染色体DNA。 该方法导致增加的转化效力和/或同源重组的频率增加。 本发明还涉及适于表达编码适合于降低至少一种染色质组件的表达或功能的染色质组装因子蛋白(例如,反义或双链RNA形式)的核酸序列的至少一部分的表达构建体 因子蛋白在植物或植物细胞中的表达。 此外,本发明的目的是重组植物细胞或植物,其包含降低的染色质组装能力,优选降低的至少一种染色质组装因子的表达或功能,以及所述重组植物用于生产食品,饲料,种子,药物或细菌的用途 化学品。