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1. 发明申请

WO2017140839A1 IMPROVED DROPLET SEQUENCING APPARATUS AND METHOD 审中-公开
标题翻译：改进的滴流测序装置和方法
公开(公告)号：WO2017140839A1
公开(公告)日：2017-08-24
申请号：PCT/EP2017/053607
申请日：2017-02-17
申请人： BASE4 INNOVATION LIMITED
发明人： FRAYLNG, Cameron Alexander , ISAAC, Thomas , SOSNA, Maciej
IPC分类号： B01J19/00 , B01L3/00 , C12Q1/68 , B01L3/02
CPC分类号： C12Q1/6869 , B01J19/00 , B01L3/0268 , B01L2200/0668 , B01L2200/142 , B01L2300/0636 , B01L2300/0829 , C12Q2563/131 , C12Q2563/137 , C12Q2563/149 , C12Q2565/301 , C12Q2565/629
摘要： A first apparatus for sequencing a polynucleotide analyte is provided an apparatus for sequencing a polynucleotide analyte comprising; • a first zone for generating a flowing stream of single nucleotides by progressive pyrophosphorolysis of a molecule of the analyte attached to a particle and exposed to a flowing aqueous medium; • a second zone for generating a corresponding stream of aqueous droplets from the aqueous medium and the nucleotide stream and wherein at least some of the droplets contain a single nucleotide and • a third zone for storing and/or for interrogating each droplet to reveal a property characteristic of the single nucleotide it may contain; characterised in that the first zone includes at least one chemically-modified substrate adapted to bind temporarily to a particle having at least two different regions adapted to bind respectively to the substrate and to the analyte. Suitably the particles comprise at least one metal coated first region(s) and at least one second region(s) comprised of reactive sites to which the analyte can be attached. In one embodiment, the two regions each comprise hemispheres of a substantially spherical bead.
摘要翻译：提供了一种用于对多核苷酸分析物进行测序的设备，其包括： ·第一区，用于通过连接至颗粒并暴露于流动的含水介质的分析物分子的渐进焦磷酸分解产生单核苷酸流动流; ·用于从含水介质和核苷酸流产生相应的含水液滴流的第二区，并且其中至少一些液滴含有单个核苷酸和·第三区用于存储和/或询问每个液滴以显示性质它可能含有的单核苷酸的特征; 其特征在于所述第一区域包括至少一个化学修饰的基材，所述化学修饰的基材适于临时结合具有至少两个不同区域的颗粒，所述至少两个不同区域适于分别结合所述基材和所述分析物。合适地，所述颗粒包含至少一个金属涂覆的第一区域和至少一个第二区域，所述第二区域包含可与分析物连接的反应位点。在一个实施例中，两个区域各自包括基本上球形的珠子的半球。

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