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1. 发明申请

WO2011115822A3 ALGAE FILTRATION SYSTEMS AND METHODS 审中-公开
公开(公告)号：WO2011115822A3
公开(公告)日：2011-09-22
申请号：PCT/US2011/028027
申请日：2011-03-11
申请人： ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY , HU, Qiang , MILTON, Sommerfeld , ZHANG, Xuezhi , KALE, Aniket
发明人： HU, Qiang , MILTON, Sommerfeld , ZHANG, Xuezhi , KALE, Aniket
IPC分类号： B01D29/62 , B01D29/23 , B01D39/00 , B01D41/00
摘要： Systems and methods for filtering algae from fluid including a piston and pressurized air system to scrape and clean algae from the filter.

2. 发明申请

WO2011127127A2 EXTRACTION WITH FRACTIONATION OF OIL AND CO-PRODUCTS FROM OLEAGINOUS MATERIAL 审中-公开
标题翻译：从油性材料中分离出油和副产物的萃取物
公开(公告)号：WO2011127127A2
公开(公告)日：2011-10-13
申请号：PCT/US2011/031353
申请日：2011-04-06
申请人： ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY , KALE, Aniket , HU, Qiang , SOMMERFELD, Milton
发明人： KALE, Aniket , HU, Qiang , SOMMERFELD, Milton
IPC分类号： C11B1/10 , C11B1/04
CPC分类号： C11B1/10 , C11B3/008
摘要： Systems and methods for extracting lipids of varying polarities from oleaginous material.
摘要翻译：从含油材料中提取不同极性的脂质的系统和方法。

3. 发明申请

WO2011127167A1 METHODS OF AND SYSTEMS FOR DEWATERING ALGAE AND RECYCLING WATER THEREFROM 审中-公开
标题翻译：用于脱水和回收水的方法和系统
公开(公告)号：WO2011127167A1
公开(公告)日：2011-10-13
申请号：PCT/US2011/031414
申请日：2011-04-06
申请人： HELIAE DEVELOPMENT, LLC , KALE, Aniket
发明人： KALE, Aniket
IPC分类号： C12N1/02 , A23J1/00
CPC分类号： C11B1/10 , B01D11/04 , C11B1/108 , Y02E50/13
摘要： A method of dewatering algae and recycling water therefrom is presented. A method of dewatering a wet algal cell culture includes removing liquid from an algal cell culture to obtain a wet algal biomass having a lower liquid content than the algal cell culture. At least a portion of the liquid removed from the algal cell culture is recycled for use in a different algal cell culture. The method includes adding a water miscible solvent set to the wet algal biomass and waiting an amount of time to permit algal cells of the algal biomass to gather and isolating at least a portion of the gathered algal cells from at least a portion of the solvent set and liquid of the wet algal biomass so that a dewatered algal biomass is generated. The dewatered algal biomass can be used to generated algal products such as biofuels and nutraceuticals.
摘要翻译：提出了一种从藻类中脱水和循环利用水的方法。对湿藻细胞培养物进行脱水的方法包括从藻类细胞培养物中除去液体以获得具有比藻细胞培养物低的液体含量的湿藻类生物质。将从藻细胞培养物中除去的至少一部分液体再循环用于不同的藻类细胞培养物中。该方法包括向湿藻类生物质中加入水混溶性溶剂组，并等待一段时间以允许藻类生物质的藻类细胞从溶剂组的至少一部分收集并分离聚集的藻类细胞的至少一部分和湿藻类生物质的液体，从而产生脱水的藻类生物质。脱水藻类生物质可用于生成藻类产品，如生物燃料和营养品。

4. 发明申请

WO2016054375A1 METHODS FOR EXTRACTING AND PURIFYING NON-DENATURED PROTEINS 审中-公开
标题翻译：提取和纯化非去除蛋白质的方法
公开(公告)号：WO2016054375A1
公开(公告)日：2016-04-07
申请号：PCT/US2015/053492
申请日：2015-10-01
申请人： IMPOSSIBLE FOODS INC. , KALE, Aniket , VARADAN, Ranjani , DAVIS, Simon Christopher
发明人： KALE, Aniket , VARADAN, Ranjani , DAVIS, Simon Christopher
IPC分类号： C12N9/88 , A61K36/81
CPC分类号： C12N9/88 , A23J1/006 , A61K36/21 , A61K2236/00 , B01D11/02 , B01D61/145 , C12Y401/01039
摘要： Materials and methods for extracting and purifying proteins are provided. For example, the materials and methods provided herein can be used for extracting and purifying proteins that denature at low temperature.
摘要翻译：提供蛋白质提取和纯化的材料和方法。例如，本文提供的材料和方法可用于提取和纯化在低温下变性的蛋白质。

5. 发明申请

WO2011127171A1 METHODS OF AND SYSTEMS FOR PRODUCING BIOFUELS 审中-公开
标题翻译：生产生物燃料的方法和系统
公开(公告)号：WO2011127171A1
公开(公告)日：2011-10-13
申请号：PCT/US2011/031419
申请日：2011-04-06
申请人： HELIAE DEVELOPMENT, LLC , KALE, Aniket
发明人： KALE, Aniket
IPC分类号： C11B1/10 , C12P7/64
CPC分类号： C11B1/10 , B01D11/04 , C11B1/108 , Y02E50/13
摘要： A method for producing biofuels is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, extracting neutral lipids from the algal biomass, and esterifying the neutral lipids with a catalyst in the presence of an alcohol. The method also includes separating a water soluble fraction comprising glycerin from a water insoluble fraction comprising fuel esters and distilling the fuel esters under vacuum to obtain a C 16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising carotenoids and omega-3 fatty acids. The method further includes hydrogenating and deoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C 16 or longer fuel esters to obtain a diesel blend stock.
摘要翻译：提供生产生物燃料的方法。制造生物燃料的方法包括使基本上完整的藻细胞脱水以制备藻类生物质，从藻类生物质中提取中性脂质，并在醇存在下用催化剂酯化中性脂质。该方法还包括从包含燃料酯的水不溶性部分中分离包含甘油的水溶性馏分，并在真空下蒸馏燃料酯以获得C 16或更短的燃料酯馏分，C16或更长的燃料酯馏分，以及包含类胡萝卜素和ω-3脂肪酸。该方法还包括将（i）C16或更短的燃料酯中的至少一种氢化和脱氧以获得喷气燃料共混料和（ii）C 16或更长的燃料酯以获得柴油混合原料。

6. 发明申请

WO2011127169A1 METHODS OF AND SYSTEMS FOR ISOLATING CAROTENOIDS AND OMEGA- 3 RICH OILS FROM ALGAE 审中-公开
标题翻译：用于分离来自阿尔卑斯的卡介质和OMEGA- 3多种油的方法和系统
公开(公告)号：WO2011127169A1
公开(公告)日：2011-10-13
申请号：PCT/US2011/031417
申请日：2011-04-06
申请人： HELIAE DEVELOPMENT, LLC , KALE, Aniket
发明人： KALE, Aniket
IPC分类号： C11B1/10 , C12P7/64
CPC分类号： C11B1/10 , B01D11/04 , C11B1/108 , Y02E50/13
摘要： A method of isolating nutraceuticals products from algae is provided. A method of isolating carotenoids and omega-3 rich oil from algae includes dewatering substantially intact algal cells to make an algal biomass and adding a first ethanol fraction to the algal biomass. The method also includes separating a first substantially solid biomass fraction from a first substantially liquid fraction comprising proteins and combining the first substantially solid biomass fraction with a second ethanol fraction. The method further includes separating a second substantially solid biomass fraction from a second substantially liquid fraction comprising polar lipids and combining the second substantially solid biomass fraction with a third ethanol solvent fraction. The method also includes separating a third substantially solid biomass fraction from a third substantially liquid fraction comprising neutral lipids, wherein the third substantially solid biomass fraction comprises carbohydrates and separating the neutral lipids into carotenoids and omega-3 rich oil.
摘要翻译：提供了一种从藻类中分离营养品的方法。从藻类中分离类胡萝卜素和富含ω-3的油的方法包括将基本上完整的藻类细胞脱水以制备藻类生物质并向藻类生物质中加入第一乙醇级分。该方法还包括从包含蛋白质的第一基本上液体的馏分中分离第一基本上固体的生物质馏分并将第一基本上固体的生物质馏分与第二乙醇馏分组合。该方法还包括从包含极性脂质的第二基本上液体的部分中分离第二基本上固体的生物质馏分，并将第二基本上固体的生物质馏分与第三乙醇溶剂馏分组合。该方法还包括从包含中性脂质的第三基本上液体的级分分离第三基本上固体的生物质馏分，其中第三基本上固体的生物质级分包含碳水化合物并将中性脂质分离成类胡萝卜素和富含ω3的油。

7. 发明申请

WO2012138438A1 METHODS OF PRODUCING BIOFUELS, CHLOROPHYLLS AND CAROTENOIDS 审中-公开
标题翻译：生产生物柴油，氯仿和卡诺菌素的方法
公开(公告)号：WO2012138438A1
公开(公告)日：2012-10-11
申请号：PCT/US2012/027537
申请日：2012-03-02
申请人： HELIAE DEVELOPMENT, LLC , KALE, Aniket
发明人： KALE, Aniket
IPC分类号： C11B1/10 , C12P7/64
CPC分类号： C11B3/10 , C10L1/026 , C10L1/04 , C10L1/08 , C11B1/02 , C11B1/10 , C11B3/008 , C11C3/003 , C12P7/6472 , Y02E50/13
摘要： A method for producing biofuels along with valuable food and neutraceutical products is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, extracting neutral lipids along with carotenoids and chlorophylls from the algal biomass, and separation of the carotenoids and chlorophylls using adsorption or membrane diafiltration or other methods. The remaining neutral lipids are esterified with a catalyst in the presence of an alcohol. The method also includes separating a water soluble fraction comprising glycerin from a water insoluble fraction comprising fuel esters and distilling the fuel esters under vacuum to obtain a C 16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising omega-3 fatty acids esters and remaining carotenoids. The method further includes hydrogenating and deoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C16 or longer fuel esters to obtain a diesel blend stock.
摘要翻译：提供了生产生物燃料的方法以及有价值的食品和药物产品。制造生物燃料的方法包括使基本上完整的藻细胞脱水以制备藻类生物质，从藻类生物质中提取中性脂质以及类胡萝卜素和叶绿素，以及使用吸附或膜渗滤或其它方法分离类胡萝卜素和叶绿素。剩余的中性脂质在醇存在下用催化剂酯化。该方法还包括从包含燃料酯的水不溶性级分中分离包含甘油的水溶性级分，并在真空下蒸馏燃料酯以获得C 16或更短的燃料酯馏分，C16或更长的燃料酯馏分，以及包含ω -3脂肪酸酯和剩余的类胡萝卜素。该方法还包括将（i）C16或更短的燃料酯中的至少一种氢化和脱氧以获得喷气燃料共混料和（ii）C16或更长的燃料酯以获得柴油混合原料。

8. 发明申请

WO2011127172A1 METHODS OF AND SYSTEMS FOR PRODUCING BIOFUELS 审中-公开
标题翻译：生产生物燃料的方法和系统
公开(公告)号：WO2011127172A1
公开(公告)日：2011-10-13
申请号：PCT/US2011/031421
申请日：2011-04-06
申请人： HELIAE DEVELOPMENT, LLC , KALE, Aniket
发明人： KALE, Aniket
IPC分类号： C11B1/10 , C12P7/64
CPC分类号： C11B1/10 , B01D11/04 , C11B1/108 , Y02E50/13
摘要： A method for producing biofuels is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, sequentially adding solvent sets to the algal biomass, and sequentially separating solid biomass fractions from liquid fractions to arrive at a liquid fraction comprising neutral lipids. The method also includes esterifying the neutral lipids, separating a water miscible fraction comprising glycerin from a water immiscible fraction comprising fuel esters, carotenoids, and omega-3 fatty acids. The method also includes obtaining a C 16 or shorter fuel esters fraction, a C 16 or longer fuel ester fraction, and a residue comprising carotenoids and omega-3 fatty acids. The method includes hydrogenating and deoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C16 or longer fuel esters to obtain a diesel blend stock.
摘要翻译：提供生产生物燃料的方法。制备生物燃料的方法包括使基本上完整的藻细胞脱水以制备藻类生物质，依次向藻类生物质中加入溶剂组，以及从液体馏分中依次分离固体生物质馏分以获得包含中性脂质的液体级分。该方法还包括酯化中性脂质，从包含燃料酯，类胡萝卜素和ω-3脂肪酸的水不混溶部分中分离包含甘油的水混溶级分。该方法还包括获得C 16或更短的燃料酯馏分，C 16或更长的燃料酯馏分和包含类胡萝卜素和ω-3脂肪酸的残余物。该方法包括将（i）C16或更短的燃料酯中的至少一种氢化和脱氧以获得喷气燃料共混料和（ii）C16或更长的燃料酯以获得柴油混合原料。

9. 发明申请

WO2011127165A2 SELECTIVE EXTRACTION OF PROTEINS FROM FRESHWATER OR SALTWATER ALGAE 审中-公开
标题翻译：从淡水或盐水中选择性提取蛋白质
公开(公告)号：WO2011127165A2
公开(公告)日：2011-10-13
申请号：PCT/US2011/031412
申请日：2011-04-06
申请人： HELIAE DEVELOPMENT, LLC , KALE, Aniket
发明人： KALE, Aniket
IPC分类号： A23J1/00
CPC分类号： C11B1/10 , B01D11/04 , C11B1/108 , Y02E50/13
摘要： Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
摘要翻译：公开了从藻类生物质或藻类培养物中选择性提取和分离藻类蛋白的方法。从藻类生物质中选择性地除去产物的方法提供允许有效分离藻类蛋白质的单步骤和多步萃取方法。这些蛋白质可用作动物原料和人类食物的可再生来源蛋白质。此外，在提取蛋白质之后残留在藻类生物质中的脂质可用于产生可再生燃料。

10. 发明申请

WO2012138382A1 EXTRACTION OF POLAR LIPIDS BY A TWO SOLVENT METHOD 审中-公开
标题翻译：通过两种溶剂方法提取极性脂肪
公开(公告)号：WO2012138382A1
公开(公告)日：2012-10-11
申请号：PCT/US2011/059152
申请日：2011-11-03
申请人： HELIAE DEVELOPMENT, LLC , KALE, Aniket
发明人： KALE, Aniket
IPC分类号： C12R1/89 , C11B1/10 , C12P7/64
CPC分类号： C11B1/10 , C11B1/108 , C12P7/6463 , C12P7/649 , Y02E50/13
摘要： A method for separating polar lipids from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting polar lipids from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal polar lipids from a wet algal biomass while avoiding emulsification of extraction mixtures. These polar lipids are high value products which can be used as surfactants, detergents, and food additives. Neutral lipids remaining in the algal biomass after extraction of polar lipids can be used to generate renewable fuels.
摘要翻译：使用两亲性溶剂组和疏水性溶剂组从植物材料，特别是完整的藻类细胞中分离极性脂质的方法。一些实施方案包括使完整的藻细胞脱水，然后从藻类细胞中提取极性脂质。该方法提供单步和多步提取方法，其允许藻类极性脂质与湿藻类生物质的有效分离，同时避免提取混合物的乳化。这些极性脂质是可用作表面活性剂，洗涤剂和食品添加剂的高价值产品。提取极性脂质后残留在藻类生物质中的中性脂质可用于产生可再生燃料。

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