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1. 发明申请

WO2011000836A1 OLIGONUCLEOTIDE HYBRIDIZATION METHOD 审中-公开
标题翻译：寡核苷酸杂交方法
公开(公告)号：WO2011000836A1
公开(公告)日：2011-01-06
申请号：PCT/EP2010/059218
申请日：2010-06-29
申请人： AIT AUSTRIAN INSTITUTE OF TECHNOLOGY GMBH , KRAINER, Siegfried , FLUCH, Silvia , LEVENTE, Bodrossy , STIERSCHNEIDER, Michael
发明人： KRAINER, Siegfried , FLUCH, Silvia , LEVENTE, Bodrossy , STIERSCHNEIDER, Michael
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/6837 , C12Q2537/161 , C12Q2527/101 , C12Q2525/107 , C12Q2527/107 , C12Q2525/121
摘要： The present invention provides a method to bind oligonucleotide molecules of a sample to an oligonucleotide probe with increased specificity comprising the steps of - providing a solid support with at least one immobilized oligonucleotide DNA probe comprising an oligonucleotide probe sequence, - in any order, contacting said probes with a sample potentially comprising oligonucleotide molecules binding said probes and contacting said probes with at least one competitive non-DNA oligonucleotide, wherein said competitive oligonucleotide comprises a sequence which is complementary to the oligonucleotide probe sequence; and/or as an alternative or in addition, in any order, contacting said probes with a sample comprising oligonucleotide molecules and contacting said oligonucleotide molecules with at least one competitive non-DNA oligonucleotide, wherein said competitive oligonucleotide comprises a sequence of the oligonucleotide probe sequence, - incubating said contacted probes with the oligonucleotide molecules of the sample and competitive oligonucleotides at a temperature of between 15°C below the melting temperature of DNA duplex oligonucleotides of the probe sequence up to said melting temperature, thereby increasing binding specificity of the oligonucleotide probe to an oligonucleotide molecule comprising a sequence complementary to said probe sequence; as well as means for performing said method.
摘要翻译：本发明提供了一种将样品的寡核苷酸分子与特异性增加的寡核苷酸探针结合的方法，包括以下步骤：提供固体支持物与至少一种包含寡核苷酸探针序列的固定寡核苷酸DNA探针， - 以任何顺序，将所述具有潜在地包含结合所述探针并使所述探针与至少一个竞争性非DNA寡核苷酸接触的寡核苷酸分子的样品的探针，其中所述竞争性寡核苷酸包含与寡核苷酸探针序列互补的序列; 和/或作为替代或另外的任何顺序，使所述探针与包含寡核苷酸分子的样品接触并使所述寡核苷酸分子与至少一个竞争性非DNA寡核苷酸接触，其中所述竞争性寡核苷酸包含寡核苷酸探针序列， - 将所述接触的探针与样品的寡核苷酸分子和竞争性寡核苷酸在比探针序列的DNA双链寡核苷酸的解链温度低15℃的温度下孵育直至所述解链温度，从而增加寡核苷酸探针的结合特异性涉及包含与所述探针序列互补的序列的寡核苷酸分子; 以及用于执行所述方法的装置。

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