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1. 发明申请

WO2010071602A1 PCR-BASED METHOD OF SYNTHESIZING A NUCLEIC ACID MOLECULE 审中-公开
标题翻译：基于PCR的合成核酸分子的方法
公开(公告)号：WO2010071602A1
公开(公告)日：2010-06-24
申请号：PCT/SG2008/000493
申请日：2008-12-19
申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH , LI, Mo-Huang , YING, Jackie Y. , YE, Hongye
发明人： LI, Mo-Huang , YING, Jackie Y. , YE, Hongye
IPC分类号： C12Q1/68 , C12N15/10 , C07H21/00 , C12P19/34
CPC分类号： C12P19/34 , C12N15/10 , C12Q1/6848 , C12Q1/686 , C12Q2527/143 , C12Q2525/143 , C12Q2525/107 , C12Q2561/113 , C12Q2547/101
摘要： There is provided a method of synthesizing a nucleic acid molecule and in one aspect, the method comprises assembling a full length template nucleic acid molecule by PCR in a PCR reaction mixture comprising a set of assembly oligonucleotides having a first average melting temperature and a set of outer amplification primers having a second average melting temperature that is lower than the first average melting temperature, wherein said assembling comprises subjecting the PCR reaction mixture to a first annealing temperature that is higher than the second average melting temperature and; amplifying the full length template nucleic acid molecule by PCR in the PCR reaction mixture wherein said amplifying comprises subjecting the PCR reaction mixture to a second annealing temperature that permits annealing of the outer amplification primers to the full length template nucleic acid molecule.
摘要翻译：提供了一种合成核酸分子的方法，一方面，该方法包括通过PCR在PCR反应混合物中组装全长模板核酸分子，所述PCR反应混合物包含一组具有第一平均熔融温度和一组具有低于第一平均熔融温度的第二平均熔融温度的外部扩增引物，其中所述组装包括使PCR反应混合物经受高于第二平均熔融温度的第一退火温度; 在PCR反应混合物中通过PCR扩增全长模板核酸分子，其中所述扩增包括使PCR反应混合物经受允许外部扩增引物退火至全长模板核酸分子的第二退火温度。

2. 发明申请

WO2017058118A1 PROCESS FOR MAKING A POLYESTER-BASED MACROINITIATOR POLYMER 审中-公开
标题翻译：制备基于聚酯的麦芽糖聚合物聚合物的方法
公开(公告)号：WO2017058118A1
公开(公告)日：2017-04-06
申请号：PCT/SG2016/050486
申请日：2016-09-30
申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
发明人： YE, Hongye , LOH, Xian Jun
IPC分类号： C08G63/46 , C08G63/47 , C08F283/02
CPC分类号： C08F283/02 , C08F2438/01 , C08G63/916 , C08F220/14
摘要： This invention relates to a method of forming a polymer comprising a step of esterifying a polyester having at least one free hydroxyl group with acyl halides or a halogen-containing carboxylic acid. The modified polyesters, made with the inventive method can be halogen-containing macroinitiator materials for further synthesis, for example, atom transfer radical polymerization as shown in other aspects of the invention. The polymers are new base materials that provide access to new Green Plastics. Articles made from the polymers are also in the scope of the invention. As an example, polyglycerol sebacate (PGS) was initially esterified into a bromine-containing macroinitiator by using bromoisobutyryl bromide (BIBB) and followed by ATRP polymerization of methyl methacrylate (MMA), thereby forming a co-polymer of PGS-g-PMMA.
摘要翻译：本发明涉及一种形成聚合物的方法，其包括使具有至少一个游离羟基的聚酯与酰卤或含卤素的羧酸酯化的步骤。用本发明方法制备的改性聚酯可以是用于进一步合成的含卤素的大分子引发剂材料，例如如本发明其它方面所示的原子转移自由基聚合。聚合物是提供新型绿色塑料的新基材。由聚合物制成的制品也在本发明的范围内。例如，通过使用溴异丁酰溴（BIBB），然后通过甲基丙烯酸甲酯（MMA）的ATRP聚合，最终将聚癸二酸甘油酯（PGS）酯化成含溴的大分子引发剂，由此形成PGS-g-PMMA的共聚物。

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