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1. 发明申请

WO2011003968A1 A FUNGAL PROTEASE AND USE THEREOF 审中-公开
标题翻译：真菌保健品及其用途
公开(公告)号：WO2011003968A1
公开(公告)日：2011-01-13
申请号：PCT/EP2010/059787
申请日：2010-07-08
申请人： AB ENZYMES OY , VALTAKARI, Leena , JUNTUNEN, Kari , MÄKINEN, Susanna , OJAPALO, Pentti , PALOHEIMO, Marja
发明人： VALTAKARI, Leena , JUNTUNEN, Kari , MÄKINEN, Susanna , OJAPALO, Pentti , PALOHEIMO, Marja
IPC分类号： C07K14/37 , C12N15/11 , C12P21/02 , C12R1/00 , C12R1/885
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme useful in modification, degradation or removal of proteinaceous material, which enzyme comprises an amino acid sequence of the mature Tr Prb 1 enzyme having an amino acid sequence of SEQ ID NO: 10 or a variant thereof having similar activity. The serine protease is obtainable from Trichoderma. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2650 comprising the nucleotide sequence SEQ ID NO: 10 of the full length enzyme deposited in E. coli RF8052 under accession number DSM 22635. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material at low or moderate temperature ranges.
摘要翻译：本发明涉及可用于蛋白质材料的修饰，降解或去除的真菌丝氨酸蛋白酶，该酶包含具有SEQ ID NO：10的氨基酸序列的成熟Tr Prb 1酶的氨基酸序列或变体具有相似的活性。丝氨酸蛋白酶可从木霉获得。还公开了编码所述蛋白酶的核酸序列，例如包含以保藏号DSM 22635在大肠杆菌RF8052中沉积的全长酶的核苷酸序列SEQ ID NO：10的质粒pALK2650。所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维，用于处理羊毛，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于涉及在低或中等温度范围内修饰，降解或除去蛋白质物质的任何应用。

2. 发明申请

WO2010125175A1 A FUNGAL SERINE PROTEASE AND USE THEREOF 审中-公开
标题翻译：真菌丝氨酸蛋白酶及其用途
公开(公告)号：WO2010125175A1
公开(公告)日：2010-11-04
申请号：PCT/EP2010/055894
申请日：2010-04-30
申请人： AB ENZYMES OY , JUNTUNEN, Kari , VALTAKARI, Leena , MÄKINEN, Susanna , KALLIO, Jarno , SZAKACS, George , VEHMAANPERÄ, Jari , OJAPALO, Pentti , PALOHEIMO, Marja
发明人： JUNTUNEN, Kari , VALTAKARI, Leena , MÄKINEN, Susanna , KALLIO, Jarno , SZAKACS, George , VEHMAANPERÄ, Jari , OJAPALO, Pentti , PALOHEIMO, Marja
IPC分类号： C12N15/80 , C07K14/37
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence the mature Fa_RF7182 enzyme having an amino acid sequence of SEQ ID NO: 18. The serine protease is obtainable from Fusarium acuminatum , more preferably from the deposited strain CBS 124084. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2530 comprising the nucleotide sequence SEQ ID NO: 12 deposited in Escherichia coli RF7803 under accession number DSM 22208 and plasmid pALK2531 comprising the full-length gene SEQ ID NO: 13 deposited in E. coli RF7879 under accession number DSM 22209, as well as fungal hosts, such as Trichoderma . Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶，其包含具有SEQ ID NO：18的氨基酸序列的成熟Fa_RF7182酶的氨基酸序列。丝氨酸蛋白酶可从尖锐镰孢获得，更优选来自保藏菌株CBS 还公开了编码所述蛋白酶的核酸序列，例如包含以保藏号DSM 22208沉积在大肠杆菌RF7803中的核苷酸序列SEQ ID NO：12的质粒pALK2530和保藏的全长基因SEQ ID NO：13的质粒pALK2531 在保藏号DSM 22209的大肠杆菌RF7879中，以及真菌宿主如木霉属（Trichoderma）。所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于任何涉及蛋白质材料的改性，降解或去除的任何应用的酶制剂。

3. 发明申请

WO2010125174A1 A NOVEL FUNGAL PROTEASE AND USE THEREOF 审中-公开
标题翻译：一种新型真菌蛋白及其用途
公开(公告)号：WO2010125174A1
公开(公告)日：2010-11-04
申请号：PCT/EP2010/055893
申请日：2010-04-30
申请人： AB ENZYMES OY , JUNTUNEN, Kari , VALTAKARI, Leena , MÄKINEN, Susanna , KALLIO, Jarno , VEHMAANPERÄ, Jari , OJAPALO, Pentti , PALOHEIMO, Marja
发明人： JUNTUNEN, Kari , VALTAKARI, Leena , MÄKINEN, Susanna , KALLIO, Jarno , VEHMAANPERÄ, Jari , OJAPALO, Pentti , PALOHEIMO, Marja
IPC分类号： C12N15/80 , C07K14/37
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti , more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO: 9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶，其包含具有SEQ ID NO：15的氨基酸序列的成熟Fe_RF6318酶的氨基酸序列。丝氨酸蛋白酶可从镰刀菌（Fusarium equiseti）获得，更优选来自沉积菌株 CBS 119568。还公开了编码所述蛋白酶的核酸序列，例如质粒pALK2521，其包含以保藏号DSM 22171保藏在大肠杆菌RF7664中的核苷酸序列SEQ ID NO：9和质粒pALK2529，质粒pALK2529包含全长基因SEQ ID NO： 10，以保藏号DSM 22172保藏在大肠杆菌RF7800中。所述蛋白酶可用作适用于洗涤剂组合物中的酶制剂，并用于处理纤维，用于处理羊毛，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于任何涉及蛋白质物质修饰，降解或去除的应用。

4. 发明申请

WO2012131023A2 PROTEASE ENZYME AND USES THEREOF 审中-公开
标题翻译：蛋白酶及其用途
公开(公告)号：WO2012131023A2
公开(公告)日：2012-10-04
申请号：PCT/EP2012/055762
申请日：2012-03-30
申请人： AB ENZYMES OY , VALTAKARI, Leena , JUNTUNEN, Kari , PALOHEIMO, Marja , OJAPALO, Pentti
发明人： VALTAKARI, Leena , JUNTUNEN, Kari , PALOHEIMO, Marja , OJAPALO, Pentti
IPC分类号： C12N9/58
CPC分类号： C11D3/386 , C11D3/2065 , C11D11/0017 , C11D11/0023 , C12N9/58 , C12Y304/21
摘要： The present invention is related to a fungal serine protease enzyme, which said enzyme has serine protease activity and comprises an amino acid sequence of Malbranchea ALKO4122 mature protease as defined in SEQ ID NO:18 or an amino acid sequence having at least 66% identity to the amino acid sequence of SEQ ID NO:18. Also disclosed is an isolated nucleic acid molecule, comprising a polynucleotide sequence which encodes a fungal serine protease enzyme, nucleic acid sequences encoding said protease, a host cell and a process of producing a polypeptide having serine protease activity. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, wool, hair, leather, or silk, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶，所述酶具有丝氨酸蛋白酶活性并且包含如SEQ ID NO：18中定义的畸枝霉属ALKO4122成熟蛋白酶的氨基酸序列或氨基酸序列序列与SEQ ID NO：18的氨基酸序列具有至少66％的同一性。还公开了分离的核酸分子，其包含编码真菌丝氨酸蛋白酶的多核苷酸序列，编码所述蛋白酶的核酸序列，宿主细胞和产生具有丝氨酸蛋白酶活性的多肽的过程。所述蛋白酶可用作可用于洗涤剂组合物中并用于处理纤维，羊毛，毛发，皮革或丝的酶制剂，用于处理食物或饲料，或用于涉及蛋白质物质的改性，降解或除去的任何应用。

5. 发明申请

WO2006032724A2 NOVEL LACCASE ENZYMES AND THEIR USES 审中-公开
标题翻译：新型漆酶及其用途
公开(公告)号：WO2006032724A2
公开(公告)日：2006-03-30
申请号：PCT/FI2005/000398
申请日：2005-09-21
申请人： AB ENZYMES OY , PALOHEIMO, Marja , PURANEN, Terhi , VALTAKARI, Leena , KRUUS, Kristiina , KALLIO, Jarno , MÄNTYLÄ, Arja , FAGERSTRÖM, Richard , OJAPALO, Pentti , VEHMAANPERÄ, Jari
发明人： PALOHEIMO, Marja , PURANEN, Terhi , VALTAKARI, Leena , KRUUS, Kristiina , KALLIO, Jarno , MÄNTYLÄ, Arja , FAGERSTRÖM, Richard , OJAPALO, Pentti , VEHMAANPERÄ, Jari
IPC分类号： C12N9/02 , C12N15/53 , D06M16/00
CPC分类号： D06M16/003 , A61K8/66 , A61Q5/00 , A61Q5/08 , C11D3/38654 , C12N9/0061 , D06L4/40 , D06P5/137 , D06P5/158 , D21C5/005 , D21H17/005
摘要： The present invention relates to novel laccase enzymes obtainable from the strains of the genus Thielavia or from the strains of the genus Chaetomium . The invention relates also to nucleic acid sequences encoding the enzymes, recombinant hosts into which the nucleic acid sequences have been introduced and to methods for the production of the enzymes in recombinant hosts. The enzymes of the invention are suitable for several applications, for example for treating denim and for strain removal.
摘要翻译：本发明涉及新的漆酶，其可得自蒂拉维氏菌属菌株或毛壳菌属菌株。本发明还涉及编码所述酶的核酸序列，其中引入了所述核酸序列的重组宿主以及在重组宿主中生产所述酶的方法。本发明的酶适用于几种应用，例如用于处理牛仔布和去除应变。

6. 发明申请

WO2006117432A1 IMPROVED CELLULASES 审中-公开
标题翻译：改进的细胞
公开(公告)号：WO2006117432A1
公开(公告)日：2006-11-09
申请号：PCT/FI2006/050161
申请日：2006-04-25
申请人： AB ENZYMES OY , VEHMAANPERÄ, Jari , PURANEN, Terhi , VALTAKARI, Leena , KALLIO, Jarno , ALAPURANEN, Marika , PALOHEIMO, Marja , OJAPALO, Pentti
发明人： VEHMAANPERÄ, Jari , PURANEN, Terhi , VALTAKARI, Leena , KALLIO, Jarno , ALAPURANEN, Marika , PALOHEIMO, Marja , OJAPALO, Pentti
IPC分类号： C12N9/42 , C12N15/62 , C12N15/63 , C12N15/80 , C11D3/386 , D06M16/00 , A23K1/165
CPC分类号： D06M16/003 , A23K10/14 , C07K2319/00 , C11D3/38645 , C12N9/2437 , C12Y302/01004 , C12Y302/01091
摘要： The present invention provides novel cellulase fusion proteins, preparations of cellulase fusion proteins and com- positions of cellulase fusion proteins. The present invention further provides cellulase expression vectors, host cells expressing cellulase and methods for preparing such vectors and cells. Uses of cellulases, cellulase preparations and cellulase compositions in the textile, detergent, pulp and paper industries are also provided.
摘要翻译：本发明提供新型纤维素酶融合蛋白，纤维素酶融合蛋白的制备和纤维素酶融合蛋白的组成。本发明还提供了纤维素酶表达载体，表达纤维素酶的宿主细胞和用于制备这种载体和细胞的方法。还提供了纤维素酶，纤维素酶制剂和纤维素酶组合物在纺织品，洗涤剂，纸浆和造纸工业中的用途。

7. 发明申请

WO2006032723A1 NOVEL LACCASE ENZYME AND USE THEREOF 审中-公开
标题翻译：新型凉菜酶及其用途
公开(公告)号：WO2006032723A1
公开(公告)日：2006-03-30
申请号：PCT/FI2005/000397
申请日：2005-09-21
申请人： AB ENZYMES OY , PALOHEIMO, Marja , VALTAKARI, Leena , PURANEN, Terhi , KRUUS, Kristiina , KALLIO, Jarno , MÄNTYLÄ, Arja , FAGERSTRÖM, Richard , OJAPALO, Pentti , VEHMAANPERÄ, Jari
发明人： PALOHEIMO, Marja , VALTAKARI, Leena , PURANEN, Terhi , KRUUS, Kristiina , KALLIO, Jarno , MÄNTYLÄ, Arja , FAGERSTRÖM, Richard , OJAPALO, Pentti , VEHMAANPERÄ, Jari
IPC分类号： C12N9/02 , C12N15/53 , D06P5/00 , C11D3/386 , D06M16/00 , A61K7/13 , D21C5/00
CPC分类号： A61Q5/00 , A61K8/66 , A61Q5/08 , C11D3/38654 , C12N9/0061 , C12Y110/03002 , D06L4/40 , D06M16/003 , D06P5/137 , D06P5/158 , D21C9/002 , D21C9/10
摘要： The present invention relates to a novel laccase enzyme obtainable from the strains of genus Thielavia. The invention relates also to the nucleic acid sequence encoding the enzyme, a recombinant host into which the nucleic acid sequence has been introduced and a method for the production of the enzyme in a recombinant host. The enzyme of the invention is suitable for several applications, in particular for increasing the lightness of denim.
摘要翻译：本发明涉及可从Thielavia属的菌株获得的新型漆酶。本发明还涉及编码该酶的核酸序列，其中引入了该核酸序列的重组宿主和在重组宿主中产生该酶的方法。本发明的酶适用于多种用途，特别是用于增加牛仔布的亮度。

8. 发明申请

WO2012055954A1 VARIANTS OF FUNGAL SERINE PROTEASE 审中-公开
标题翻译：真菌丝氨酸蛋白酶的变体
公开(公告)号：WO2012055954A1
公开(公告)日：2012-05-03
申请号：PCT/EP2011/068837
申请日：2011-10-27
申请人： AB ENZYMES OY , JUNTUNEN, Kari , VALTAKARI, Leena , HAKULINEN, Nina , PALOHEIMO, Marja
发明人： JUNTUNEN, Kari , VALTAKARI, Leena , HAKULINEN, Nina , PALOHEIMO, Marja
IPC分类号： C12N9/58 , C11D3/386
CPC分类号： C12N9/58 , C11D3/38609 , C11D3/38618
摘要： The present invention relates to fungal serine protease variants, which comprise an amino acid substitution of valine at position 208 of the parent Fusarium equiseti Fe_RF6318 serine protease, wherein the position of the substitution corresponds to the amino acid sequence of the mature Fe_RF6318 enzyme defined in SEQ ID NO: 2. The variants have improved thermal stability and/or detergent stability compared to the parent Fe_RF6318 enzyme. Preferably the substitution is V208I and more preferably the variants comprise additional amino acid changes which further increase the stability. Also disclosed are nucleic acid sequences encoding said protease variants as well as recombinant vectors and host cells for the production of the variants. The serine protease variants are applicable in laundry and dish-washing detergent compositions, in treating fibers, in treating wool, in treating hair, in treating leather, in treating food or feed, or in any application involving modification, degradation or removal of proteinaceous material.
摘要翻译：本发明涉及真菌丝氨酸蛋白酶变体，其包含母本镰刀菌属Fe_RF6318丝氨酸蛋白酶的208位的缬氨酸的氨基酸取代，其中取代的位置对应于SEQ ID NO：1中定义的成熟Fe_RF6318酶的氨基酸序列所述变体与母体Fe_RF6318酶相比具有改善的热稳定性和/或洗涤剂稳定性。优选地，取代是V208I，更优选地，变体包含进一步增加稳定性的另外的氨基酸变化。还公开了编码所述蛋白酶变体的核酸序列以及用于产生变体的重组载体和宿主细胞。丝氨酸蛋白酶变体可用于洗衣和洗碟洗涤剂组合物，处理纤维，处理羊毛，处理毛发，处理皮革，处理食品或饲料，或涉及任何涉及蛋白质物质的改性，降解或去除的应用。

9. 发明申请

WO2005100557A1 METHOD AND DNA CONSTRUCTS FOR INCREASING THE PRODUCTION LEVEL OF CARBOHYDRATE DEGRADING ENZYMES IN FILAMENTOUS FUNGI 审中-公开
标题翻译：用于增加真菌真菌中碳酸氢盐降解酶生产水平的方法和DNA构建
公开(公告)号：WO2005100557A1
公开(公告)日：2005-10-27
申请号：PCT/FI2005/050123
申请日：2005-04-15
申请人： AB ENZYMES OY , PALOHEIMO, Marja , MÄNTYLÄ, Arja , LESKINEN, Sanna , FAGERSTRÖM, Richard , KALLIO, Jarno , PURANEN, Terhi , LANTTO, Raija , SUOMINEN, Pirkko
发明人： PALOHEIMO, Marja , MÄNTYLÄ, Arja , LESKINEN, Sanna , FAGERSTRÖM, Richard , KALLIO, Jarno , PURANEN, Terhi , LANTTO, Raija , SUOMINEN, Pirkko
IPC分类号： C12N9/24
CPC分类号： D21C5/005 , C12N9/248 , C12N15/80 , C12Y302/01008 , D21H17/005
摘要： The invention is related to a method and DNA constructs for obtaining in a filamentous fungus host a higher production level of a carbohydrate degrading (CD) enzyme, having in its original state a catalytic module (CAT) and a carbohydrate binding module (CBM) separated by a linker region. The DNA construct comprising a truncated actinomycetes, preferably Nonomuraea flexuosa (Nf) derived DNA sequence encoding a truncated form of the CD enzyme, for example Nf Xyn1 1A, Nf Xyn10A, and is introduced into a filamentous fungal host. Said CD enzyme contains the catalytically active region of CAT but lacks part or all of the CBM, or all of the CBM and part or all of the linker region and is expressed and secreted under the control of regulatory sequences comprising at least a signal sequence, but also promoters, terminators and DNA sequences encoding a secretable carrier protein or domains thereof, preferably originating from filamentous fungi are included. The production level obtained with DNA sequence having the shortened DNA sequence encoding the truncated form of the CD enzyme is higher than the production level obtained with DNA construct encoding the corresponding full length CD enzyme.
摘要翻译：本发明涉及用于在丝状真菌宿主中获得更高生产水平的降解碳水化合物（CD）酶的方法和DNA构建体，其具有在其初始状态下分离的催化模块（CAT）和碳水化合物结合模块（CBM）通过接头区域。 DNA构建体包含截短的放线菌，优选非编杆菌（Nf）衍生的编码CD酶的截短形式的DNA序列，例如Nf Xyn1A1，NfXyn10A，并引入丝状真菌宿主中。所述CD酶含有CAT的催化活性区域，但缺少部分或全部CBM或全部CBM和部分或全部接头区域，并且在包含至少一个信号序列的调节序列的控制下表达和分泌，而且还包括编码可分泌载体蛋白或其结构域的启动子，终止子和DNA序列，优选来源于丝状真菌。用缩短的编码CD酶的截短形式的DNA序列的DNA序列获得的生产水平高于用编码相应的全长CD酶的DNA构建体获得的产生水平。

10. 发明申请

WO2022144500A1 XYLANASE VARIANTS 审中-公开
公开(公告)号：WO2022144500A1
公开(公告)日：2022-07-07
申请号：PCT/FI2021/050911
申请日：2021-12-23
申请人： AB ENZYMES OY
发明人： PALOHEIMO, Marja , JUNTUNEN, Kari , TURUNEN, Ossi , AHOLA, Pihla , TAIPALUS, Pasi , MÜLLER, Eugen , SAVOLAINEN, Jukka , LANGFELDER, Kim , PURANEN, Terhi
IPC分类号： C12N9/24 , C12P19/14 , D21C5/00
摘要： A variant polypeptide of xylanase, and a fusion protein and an enzyme composition comprising the variant polypeptide, a recombinant host cell for production of the variant polypeptide, methods for using it and its use, is disclosed comprising an amino acid sequence having at least 79 %, but less than 100 % amino acid sequence identity with amino acids 1-191 of SEQ ID NO: 1, wherein the amino acid sequence has xylanase activity, at least one disulfide bridge between two Cys residues in the 1-191 amino acid region, and an amino acid substitution at the position 23 or 28, or at the positions 23 and 28, the positions corresponding to the positions 23 and 28 of the SEQ ID NO: 1.

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