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1. 发明申请

WO2010101931A2 DETECTION SYSTEM AND METHOD FOR HIGH SENSITIVITY FLUORESCENT ASSAYS 审中-公开
标题翻译：检测系统和高灵敏度荧光测定方法
公开(公告)号：WO2010101931A2
公开(公告)日：2010-09-10
申请号：PCT/US2010025938
申请日：2010-03-02
申请人： ACCESS MEDICAL SYSTEM CO LTD , TAN HONG , ZUK ROBERT F , TAN YUSHAN , CAO ERHUA , XIA MIN , CHEN JUN
发明人： TAN HONG , ZUK ROBERT F , TAN YUSHAN , CAO ERHUA , XIA MIN , CHEN JUN
IPC分类号： G01N21/64 , G01N33/487 , G01N33/50
CPC分类号： G01N33/54366 , C08B37/006 , G01N21/6428 , G01N21/645 , G01N33/54373 , G01N2021/6439 , G01N2201/06113
摘要： This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area ( =5 mm) and a high molecular weight polymer ( =1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked Ficoll molecule having a plurality of binding molecules and a plurality of fluorescent labels.
摘要翻译：本发明涉及一种用于在荧光测定中测量荧光信号的检测系统。该系统包括具有与荧光标签结合的小感测表面的探针，以及安装在基底的感测表面的近侧的光源和检测器。本发明还涉及使用具有小表面积（= 5mm）和具有多个结合分子和多个荧光标记的高分子量聚合物（= 1M）的探针尖端来检测液体样品中的分析物的方法。通过横向流动反应溶液并在反应容器中上下移动探针尖端来加速结合反应。本发明还涉及包含具有多个结合分子和多个荧光标记的交联Ficoll分子的荧光标记组合物。

2. 发明申请

WO2012145270A3 LUMINESCENT POLYMER CYCLIC AMPLIFICATION 审中-公开
标题翻译：发光聚合物循环放大
公开(公告)号：WO2012145270A3
公开(公告)日：2012-12-20
申请号：PCT/US2012033808
申请日：2012-04-16
申请人： ACCESS MEDICAL SYSTEMS LTD , ZUK ROBERT F , TAN HONG
发明人： ZUK ROBERT F , TAN HONG
IPC分类号： G01N33/53 , G01N33/52 , G01N33/533
CPC分类号： G01N33/54393 , G01N21/76 , G01N27/3277 , G01N33/5306 , G01N33/533 , G01N33/5438 , G01N33/553 , G01N2021/757
摘要： The present invention is directed to a luminescent immunoassay method for detecting an analyte in a liquid sample with high sensitivity. The invention provides a unique combination of (i) using a probe having a small sensing surface area for binding analyte molecules, (ii) using a high molecular weight branched polymer conjugated with multiple binding molecules and multiple luminescent labels, and (iii) cycling the probe having immunocomplex formed back to the reagent vessel and amplification vessel 1-10 times and repeating the reaction with the reagent and the amplification polymer, to improve the sensitivity of detection level. For each cycling, the luminescent signal is increased significantly over the noise.
摘要翻译：本发明涉及用于以高灵敏度检测液体样品中的分析物的发光免疫测定方法。本发明提供了以下独特的组合：（i）使用具有小的感测表面积的探针来结合分析物分子，（ii）使用与多个结合分子和多个发光标记物缀合的高分子量支化聚合物，和（iii）探针将免疫复合物形成回到试剂容器和扩增容器1-10次，并重复与试剂和扩增聚合物的反应，以提高检测水平的灵敏度。对于每次循环，发光信号在噪音上显着增加。

3. 发明申请

WO2012094625A2 SYSTEMS FOR IMMUNOASSAY TESTS 审中-公开
标题翻译：免疫测试系统
公开(公告)号：WO2012094625A2
公开(公告)日：2012-07-12
申请号：PCT/US2012/020532
申请日：2012-01-06
申请人： ACCESS MEDICAL SYSTEMS, LTD. , TAN, Hong , XIA, Ming , TAN, Yushan , CHEN, Jun , CAO, Erhua , LI, Genqian , ZUK, Robert F.
发明人： TAN, Hong , XIA, Ming , TAN, Yushan , CHEN, Jun , CAO, Erhua , LI, Genqian , ZUK, Robert F.
IPC分类号： G01N33/53 , G01N33/532 , G01N33/52 , G01N33/543
CPC分类号： B01L3/52 , B01L3/502 , B01L3/527 , B01L2300/042 , B01L2300/045 , G01N33/54306 , G01N33/54353 , G01N33/54366 , G01N35/026 , G01N2035/0436
摘要： This invention relates to a cartridge for an immunoassay test. The cartridge comprises (a) a probe well comprising a probe and a cap, the cap being in a closed position to enclose the probe in the probe well, wherein the probe has a bottom tip coated with analyte-binding molecules; (b) a sample well to receive a sample; (c) one or more reagent wells; (d) a plurality of wash wells each containing a first aqueous solution; and (e) a measurement well having a light transmissive bottom, the measurement well containing a second aqueous solution; wherein the openings of the sample well, reagent well, measurement well and wash wells are sealed. The present invention also relates to an apparatus for loading and releasing a probe. The apparatus comprises a push pin and a groove to load and transfer the probe to a plurality of locations, such as different wells in the above-mentioned cartridge, to conduct the immunoassay test.
摘要翻译：本发明涉及用于免疫测定试验的药筒。所述筒包括（a）包括探针和帽的探针孔，所述盖处于闭合位置以将所述探针包围在探针中，其中所述探针具有涂覆有分析物结合分子的底部尖端; （b）样品孔以接收样品; （c）一个或多个试剂孔; （d）多个洗涤孔，每个洗涤孔含有第一水溶液; 和（e）具有透光底部的测量井，所述测量井含有第二水溶液; 其中样品阱，试剂阱，测量井和洗涤井的开口被密封。本发明还涉及一种用于装载和释放探头的装置。该装置包括推针和凹槽，以将探针加载并转移到多个位置，例如上述药筒中的不同的孔，以进行免疫测定。

4. 发明申请

WO2010101931A3 DETECTION SYSTEM AND METHOD FOR HIGH SENSITIVITY FLUORESCENT ASSAYS 审中-公开
公开(公告)号：WO2010101931A3
公开(公告)日：2010-09-10
申请号：PCT/US2010/025938
申请日：2010-03-02
申请人： ACCESS MEDICAL SYSTEM CO., LTD. , TAN, Hong , ZUK, Robert F. , TAN, Yushan , CAO, Erhua , XIA, Min , CHEN, Jun
发明人： TAN, Hong , ZUK, Robert F. , TAN, Yushan , CAO, Erhua , XIA, Min , CHEN, Jun
IPC分类号： G01N21/64 , G01N33/487 , G01N33/50
摘要： This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area ( ≤5 mm) and a high molecular weight polymer ( ≥1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked Ficoll molecule having a plurality of binding molecules and a plurality of fluorescent labels.

5. 发明申请

WO2009102794A1 LANCET WITH SHIELDED LANCE 审中-公开
标题翻译：带屏蔽LANCET
公开(公告)号：WO2009102794A1
公开(公告)日：2009-08-20
申请号：PCT/US2009/033803
申请日：2009-02-11
申请人： ZUK, Robert, F. , TAN, Hong
发明人： ZUK, Robert, F. , TAN, Hong
IPC分类号： A61B17/14 , A61B17/32
CPC分类号： A61B5/157 , A61B5/150022 , A61B5/150358 , A61B5/150435 , A61B5/150519 , A61B5/150664 , A61B5/150702 , A61B5/15113 , A61B5/15117 , A61B5/15186 , A61B5/1519 , A61B5/15194
摘要： An improved lancet as well as methods and devices for using the improved lancet are provided. The improved lancet comprises a body section with a lance arm and one or more protective arms extending from the body section. The lance arm has a lance with a sharp portion for piercing skin disposed on the lance arm. The sharp portion of the lance extends at an angle of about 45 to 135 degrees to the direction of extension of the lance arm from the body section. The protective arms have a protective section for shielding the sharp portion of the lance. The sharp portion of the lance is exposed from the protective section by application of pressure to the lance arm.
摘要翻译：提供了一种改进的柳叶刀以及使用改进的柳叶刀的方法和装置。改进的柳叶刀包括具有矛杆臂的主体部分和从主体部分延伸的一个或多个保护臂。喷枪臂具有用于刺穿布置在喷枪上的皮肤的尖锐部分的喷枪。喷枪的尖锐部分与喷枪臂从主体部分延伸的方向以大约45度至135度的角度延伸。保护臂具有用于屏蔽喷枪尖锐部分的保护部分。喷枪的尖锐部分通过对喷枪臂施加压力而从保护部分暴露出来。

6. 发明申请

WO1997008556A1 ANALYTICAL ROTOR AND METHOD FOR DETECTING ANALYTES IN LIQUID SAMPLES 审中-公开
标题翻译：用于检测液体样品中分析物的分析转子和方法
公开(公告)号：WO1997008556A1
公开(公告)日：1997-03-06
申请号：PCT/US1996014151
申请日：1996-08-27
申请人： FIRST MEDICAL, INC. , ELLSWORTH, Stoughton, L. , KARUNARATNE, Arjuna, R. , PIERCE, Jeffrey, A. , ZUK, Robert, F. , GUSTAFSON, Eric, K. , SUBRAMANIAN, Kumar
发明人： FIRST MEDICAL, INC.
IPC分类号： G01N33/543
CPC分类号： G01N21/07
摘要： An analytical rotor for performing immunoassays comprising one or more inlet chambers (16) for sample, wash reagents, and labelling reagents. A reaction chamber (28) is disposed radially outwardly from the inlet chambers and connected thereto by low flow resistance flow paths (34). A collection chamber (60) is located radially outwardly from the reaction chamber and connected thereto by a high flow resistance flow path (62). Samples are introduced to the sample inlet chamber by a transfer device, with sample volumes optionally determined by detecting when the sample inlet chamber is filled. Reagents initially introduced to the inlet chambers may be selectively transferred to the reaction chamber by low speed rotation of the rotor. The reaction chamber may then be emptied by high speed rotation of the rotor. In this way, heterogeneous immunoassays requiring sequential contact of reaction zones with sample and different reagents may be performed.
摘要翻译：一种用于进行免疫测定的分析转子，包括用于样品，洗涤试剂和标记试剂的一个或多个入口室（16）。反应室（28）从入口室径向向外设置并通过低流阻流路（34）与其连接。收集室（60）从反应室径向向外定位，并通过高流动阻力流动路径（62）与其连接。通过转移装置将样品引入样品入口室，任选地通过检测样品入口室何时填充样品量来确定样品体积。最初引入入口室的试剂可以通过转子的低速旋转选择性地转移到反应室。然后可以通过转子的高速旋转将反应室排空。以这种方式，可以进行需要与样品和不同试剂连续接触反应区的多相免疫测定。

7. 发明申请

WO2012145270A2 LUMINESCENT POLYMER CYCLIC AMPLIFICATION 审中-公开
标题翻译： LUMINESCENT聚合物循环放大
公开(公告)号：WO2012145270A2
公开(公告)日：2012-10-26
申请号：PCT/US2012/033808
申请日：2012-04-16
申请人： ACCESS MEDICAL SYSTEMS, LTD. , ZUK, Robert F. , TAN, Hong
发明人： ZUK, Robert F. , TAN, Hong
IPC分类号： G01N33/53 , G01N33/52 , G01N33/533
CPC分类号： G01N33/54393 , G01N21/76 , G01N27/3277 , G01N33/5306 , G01N33/533 , G01N33/5438 , G01N33/553 , G01N2021/757
摘要： The present invention is directed to a luminescent immunoassay method for detecting an analyte in a liquid sample with high sensitivity. The invention provides a unique combination of (i) using a probe having a small sensing surface area for binding analyte molecules, (ii) using a high molecular weight branched polymer conjugated with multiple binding molecules and multiple luminescent labels, and (iii) cycling the probe having immunocomplex formed back to the reagent vessel and amplification vessel 1-10 times and repeating the reaction with the reagent and the amplification polymer, to improve the sensitivity of detection level. For each cycling, the luminescent signal is increased significantly over the noise.
摘要翻译：本发明涉及一种用于以高灵敏度检测液体样品中的分析物的发光免疫测定方法。本发明提供了以下独特的组合：（i）使用具有小的感测表面积的探针来结合分析物分子，（ii）使用与多个结合分子共轭的高分子量支化聚合物和多个发光标记，以及（iii）将具有免疫复合物的探针形成回试剂容器和扩增容器1-10次，并重复与试剂和扩增聚合物的反应，以提高检测水平的灵敏度。对于每个循环，发光信号在噪声上显着增加。

8. 发明申请

WO2010096702A3 OPTICAL SENSOR OF BIO-MOLECULES USING THIN-FILM INTERFEROMETER 审中-公开
标题翻译：使用薄膜干涉仪的生物分子光学传感器
公开(公告)号：WO2010096702A3
公开(公告)日：2011-01-27
申请号：PCT/US2010024801
申请日：2010-02-19
申请人： ACCESS MEDICAL SYSTEM CO LTD , TAN HONG , TAN YUSHAN , CAO ERHUA , XIA MIN , ZUK ROBERT F
发明人： TAN HONG , TAN YUSHAN , CAO ERHUA , XIA MIN , ZUK ROBERT F
IPC分类号： G01N21/25 , G01B9/02 , G01N21/45 , G01N33/483
CPC分类号： G01N21/7703 , G01J3/45 , G01N21/45 , G01N21/648 , G01N21/8507 , G01N2021/7779 , G01N2201/062 , G01N2201/08 , G02B6/262 , G02B6/30
摘要： The present invention is directed to an assembly for use in detecting an analyte in a sample based on thin-film spectral interference. The assembly comprises a waveguide, a monolithic substrate optically coupled to the waveguide, and a thin-film layer directly bonded to the sensing side of the monolithic substrate. The refractive index of the monolithic substrate is higher than the refractive index of the transparent material of the thin-film layer. A spectral interference between the light reflected into the waveguide from a first reflecting surface and a second reflecting surface varies as analyte molecules in a sample bind to the analyte binding molecules coated on the thin-film layer.
摘要翻译：本发明涉及一种用于基于薄膜光谱干涉检测样品中的分析物的组件。组件包括波导，光耦合到波导的单片基板和直接结合到整体式基板的感测侧的薄膜层。单片基板的折射率高于薄膜层的透明材料的折射率。当样品中的分析物分子结合到涂覆在薄膜层上的分析物结合分子时，从第一反射表面和第二反射表面反射到波导中的光之间的光谱干涉变化。

9. 发明申请

WO1997008552A1 FLUORESCENT AND LUMINESCENT LABELLING COMPOSITIONS AND METHODS FOR THEIR USE 审中-公开
标题翻译：荧光和发光标签组合物及其使用方法
公开(公告)号：WO1997008552A1
公开(公告)日：1997-03-06
申请号：PCT/US1996014025
申请日：1996-08-26
申请人： FIRST MEDICAL, INC. , ZUK, Robert, F. , CHOO, Sae, Hyun
发明人： FIRST MEDICAL, INC.
IPC分类号： G01N33/53
CPC分类号： G01N33/533 , Y10S435/96 , Y10S435/968 , Y10S436/80
摘要： Fluorescent and chemiluminescent labelling compositions comprise a linear polysaccharide backbone molecule having a plurality of target-binding molecules, such as antibodies or nucleic acids, attached at spaced-apart intervals thereon. Each of the target-binding molecules, in turn, includes a multiplicity of fluorescent or chemiluminescent dye molecules bound thereto. In this way, signal introduced to a single target-site on a solid phase surface may be increased without loss of binding activity.
摘要翻译：荧光和化学发光标记组合物包含线性多糖骨架分子，其具有以间隔隔开间隔连接的多个靶结合分子，例如抗体或核酸。靶结合分子中的每一个又包括与其结合的多个荧光或化学发光染料分子。以这种方式，可以增加引入固相表面上的单个靶位点的信号而不损失结合活性。

10. 发明申请

WO1990004786A1 HAPTEN DERIVATIZED CAPTURE MEMBRANE AND DIAGNOSTIC ASSAYS USING SUCH MEMBRANE 审中-公开
标题翻译：使用这种膜的HAPTEN衍生的捕获膜和诊断测定
公开(公告)号：WO1990004786A1
公开(公告)日：1990-05-03
申请号：PCT/US1989004320
申请日：1989-10-10
申请人： MOLECULAR DEVICES CORPORATION , OLSON, John, D. , ZUK, Robert, F. , ARMENTA, Richard, D. , BURKE, Charles, R. , KUNG, Viola, T. , SHELDON, Edward, L., III
发明人： MOLECULAR DEVICES CORPORATION
IPC分类号： G01N33/53
CPC分类号： G01N33/54353 , C07H21/00 , C12Q1/6804 , C12Q1/6816 , C12Q1/6834 , C12Q1/686 , C12Q1/6876 , G01N33/537 , G01N33/54306 , C12Q2563/131 , C12Q2522/101
摘要： The present invention encompasses a capture membrane comprising a porous filter membrane having a hapten bound directly or indirectly to the membrane wherein complexes formed by specific binding having an anti-hapten bound to a binding member of the specifically binding complex are removed from a solution by the hapten as the solution passes through the membrane. In the preferred embodiment biotin is the hapten and avidin or streptavidin is the anti-hapten.
摘要翻译：本发明包括捕获膜，其包含具有直接或间接结合膜的半抗原的多孔过滤膜，其中通过特异性结合形成的具有与结合特异性结合复合物的结合成员结合的抗半抗原形成的复合物通过半抗原溶液通过膜。在优选实施方案中，生物素是半抗原，抗生物素蛋白或链霉亲和素是抗半抗原。

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