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1. 发明申请

WO2005070180A2 COCCIDIAN PARASITE CASEIN KINASE I AS A CHEMOTHERAPEUTIC TARGET FOR ANTIPROTOZOAL AGENTS 审中-公开
标题翻译： COCCIDIAN PARASITE CASEIN KINASE I作为抗真菌药物的化学靶向
公开(公告)号：WO2005070180A2
公开(公告)日：2005-08-04
申请号：PCT/US2005000955
申请日：2005-01-12
申请人： MERCK & CO INC , DONALD ROBERT G K , LIBERATOR PAUL , ZHONG XIAOTIAN
发明人： DONALD ROBERT G K , LIBERATOR PAUL , ZHONG XIAOTIAN
IPC分类号： C07K14/45 , C07K14/455 , C12N9/12
CPC分类号： C07K14/45 , C07K14/455 , C12N9/12
摘要： Isolated nucleic acid molecules encoding coccidian casein kinase I, CKI, enzymes from the species Eimeria tenella and Toxoplasma gondii are disclosed. The isolation of these coccidian CKI cDNA sequences results in the disclosure of purified forms of E. tenella and T. gondii CKI proteins, recombinant vectors and recombinant hosts which express coccidian CKI.
摘要翻译：公开了编码球虫酪蛋白激酶I，CKI，来自艾美球虫（Eimeria tenella）和弓形虫（弓形虫）的酶的分离的核酸分子。这些球虫CKI cDNA序列的分离导致纯化形式的E. tenella和弓形虫CKI蛋白，重组载体和表达球虫CKI的重组宿主的公开。

2. 发明申请

WO2008060307A2 METHODS OF LABELING TRANSIENTLY EXPRESSED PROTEINS IN LARGE-SCALE EUKARYOTIC CELL CULTURES 审中-公开
标题翻译：在大型真核细胞培养基中标记瞬时表达的蛋白质的方法
公开(公告)号：WO2008060307A2
公开(公告)日：2008-05-22
申请号：PCT/US2007/000336
申请日：2007-01-09
申请人： WYETH , ZHONG, Xiaotian , KRIZ, Ronald, William
发明人： ZHONG, Xiaotian , KRIZ, Ronald, William
CPC分类号： C07K1/13 , C12N9/12 , C12P21/02
摘要： The present invention is based on observations that transiently transfecting large- scale eukaryotic cell cultures with a polynucleotide encoding a protein of interest can be used to rapidly produce the large quantities of labeled proteins required for various biochemical techniques such as spectroscopy, microscopy, and crystallography, and applications including protein structure determination, protein tracing and/or localization, diagnostic and therapeutic applications, and affinity experiments. Thus, the present invention provides methods for rapidly producing large quantities of labeled proteins by using transient transfection of large-scale eukaryotic cell cultures, which are then grown in a chemically defined labeling medium that includes labeled amino acids. The present invention is also directed to methods of using the labeled proteins produced by the novel labeling methods for use in various techniques.
摘要翻译：本发明基于以下观察结果，即用编码感兴趣蛋白质的多核苷酸瞬时转染大规模真核细胞培养物可用于快速产生各种生物化学技术所需的大量标记蛋白质如光谱学，显微镜和晶体学，以及包括蛋白质结构测定，蛋白质追踪和/或定位，诊断和治疗应用以及亲和力实验在内的应用。因此，本发明提供了通过使用大规模真核细胞培养物的瞬时转染来快速产生大量标记蛋白质的方法，所述大规模真核细胞培养物然后在包含标记的氨基酸的化学确定的标记介质中生长。本发明还涉及使用由新颖标记方法产生的标记蛋白质用于各种技术的方法。

3. 发明申请

WO2006113861A2 MAMMALIAN EXPRESSION SYSTEMS 审中-公开
标题翻译： MAMMALIAN表达系统
公开(公告)号：WO2006113861A2
公开(公告)日：2006-10-26
申请号：PCT/US2006014859
申请日：2006-04-20
申请人： WYETH CORP , ZHONG XIAOTIAN , KRIZ RONALD , STAHL MARK
发明人： ZHONG XIAOTIAN , KRIZ RONALD , STAHL MARK
IPC分类号： C12N5/06 , C07K14/71 , C08B37/10
CPC分类号： C12P21/02
摘要： The present invention features mammalian expression systems with improved production yields, and method of using these systems to produce desired proteins. In one embodiment, the expression systems of the present invention comprise genetically-engineered mammalian host cells cultured in a medium that contains an effective amount of heparin or heparin-like molecules. The presence of heparin or heparin-like molecules significantly increases protein production by the cultured cells. The present invention also features the use of constitutively-active components of FGFR-I -mediated signal transduction pathways to improve protein production by cultured mammalian cells. Co-expression of such a component with a protein of interest markedly increases the production yield of the protein of interest.
摘要翻译：本发明具有提高生产产量的哺乳动物表达系统以及使用这些系统产生所需蛋白质的方法。在一个实施方案中，本发明的表达系统包含在含有有效量的肝素或类肝素分子的培养基中培养的遗传工程化的哺乳动物宿主细胞。肝素或肝素样分子的存在显着增加培养细胞的蛋白质产生。本发明还特征在于使用FGFR-1介导的信号转导途径的组成型活性成分来改善培养的哺乳动物细胞的蛋白质产生。这种与感兴趣的蛋白质的组分的共表达显着增加了目的蛋白质的产量。

4. 发明申请

WO2006028889A2 SYSTEMS AND METHODS FOR PROTEIN PRODUCTION 审中-公开
标题翻译：蛋白质生产的系统和方法
公开(公告)号：WO2006028889A2
公开(公告)日：2006-03-16
申请号：PCT/US2005/031081
申请日：2005-09-01
申请人： WYETH , GAO, Yijie , PICHE, Nicole, M. , GENG, Mei , HERRMANN, Stephen, H. , ZHONG, Xiaotian , KRIZ, Ronald , LU, Zhijian
发明人： GAO, Yijie , PICHE, Nicole, M. , GENG, Mei , HERRMANN, Stephen, H. , ZHONG, Xiaotian , KRIZ, Ronald , LU, Zhijian
CPC分类号： C07K14/4705 , A01K2217/075 , C07K14/51 , C12P21/02
摘要： The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells aare animal cells, and the co-expressed polypeptide is a component or modulator of an XBP1- or ATF6- mediated UPR pathway.
摘要翻译：本发明涉及生产目标蛋白质的系统和方法。本发明使用具有改变的蛋白质折叠或加工能力的遗传工程化的动物或植物细胞。一方面，本发明的特征在于遗传工程改造的细胞，其包含编码（1）目的蛋白质的一种或多种重组表达盒，和（2）在细胞的未折叠蛋白质应答（UPR）途径中有功能的多肽。多肽的共表达显着提高了遗传工程细胞中目标蛋白的产量。在一个实例中，遗传工程改造的细胞是动物细胞，共表达的多肽是XBP1或ATF6介导的UPR途径的组分或调节剂。

5. 发明申请

WO2008060307A3 METHODS OF LABELING TRANSIENTLY EXPRESSED PROTEINS IN LARGE-SCALE EUKARYOTIC CELL CULTURES 审中-公开
标题翻译：在大规模的真核细胞培养物中标记瞬时表达蛋白的方法
公开(公告)号：WO2008060307A3
公开(公告)日：2008-10-09
申请号：PCT/US2007000336
申请日：2007-01-09
申请人： WYETH CORP , ZHONG XIAOTIAN , KRIZ RONALD WILLIAM
发明人： ZHONG XIAOTIAN , KRIZ RONALD WILLIAM
IPC分类号： C07K1/13 , C12N9/12 , C12P21/02
CPC分类号： C07K1/13 , C12N9/12 , C12P21/02
摘要： The present invention is based on observations that transiently transfecting large- scale eukaryotic cell cultures with a polynucleotide encoding a protein of interest can be used to rapidly produce the large quantities of labeled proteins required for various biochemical techniques such as spectroscopy, microscopy, and crystallography, and applications including protein structure determination, protein tracing and/or localization, diagnostic and therapeutic applications, and affinity experiments. Thus, the present invention provides methods for rapidly producing large quantities of labeled proteins by using transient transfection of large-scale eukaryotic cell cultures, which are then grown in a chemically defined labeling medium that includes labeled amino acids. The present invention is also directed to methods of using the labeled proteins produced by the novel labeling methods for use in various techniques.
摘要翻译：本发明基于以编码目的蛋白质的多核苷酸瞬时转染大规模真核细胞培养物的观察结果，可用于快速产生各种生物化学技术所需的大量标记蛋白质，如光谱学，显微镜和晶体学，以及包括蛋白质结构测定，蛋白质追踪和/或定位，诊断和治疗应用以及亲和力实验的应用。因此，本发明提供了通过使用大规模真核细胞培养物的瞬时转染来快速产生大量标记的蛋白质的方法，然后将其在包括标记的氨基酸的化学学定义的标记培养基中生长。本发明还涉及使用通过新型标记方法产生的标记蛋白用于各种技术的方法。

6. 发明申请

WO2007025248A3 AGGRECANASE STRUCTURE 审中-公开
公开(公告)号：WO2007025248A3
公开(公告)日：2007-03-01
申请号：PCT/US2006/033498
申请日：2006-08-24
申请人： WYETH , MOSYAK, Lidia , RUSH III, Thomas Saltmarsh , ZHONG, Xiaotian , MCDONAGH, Thomas E. , GEORGIADIS, Katy E. , SUM, Phaik-Eng , LAVALLIE, Edward R. , COLLINS-RACIE, Lisa A. , CORCORAN, Christopher John , KUMAR, Ravindra , HEBERT, Tracy , OLLAND, Stephane Hubert , MACKIE, Stewart Andrews
发明人： MOSYAK, Lidia , RUSH III, Thomas Saltmarsh , ZHONG, Xiaotian , MCDONAGH, Thomas E. , GEORGIADIS, Katy E. , SUM, Phaik-Eng , LAVALLIE, Edward R. , COLLINS-RACIE, Lisa A. , CORCORAN, Christopher John , KUMAR, Ravindra , HEBERT, Tracy , OLLAND, Stephane Hubert , MACKIE, Stewart Andrews
IPC分类号： C12N9/64 , G06F19/00 , G01N23/20 , C30B7/00
摘要： This invention relates to aggrecanase polypeptides and aggrecanase polypeptide/ligand complexes, crystals of aggrecanase and aggrecanase polypeptide/ligand complexes, and related methods and software systems.

7. 发明申请

WO2007025248B1 AGGRECANASE STRUCTURE 审中-公开
公开(公告)号：WO2007025248B1
公开(公告)日：2007-10-11
申请号：PCT/US2006033498
申请日：2006-08-24
申请人： WYETH CORP , MOSYAK LIDIA , RUSH III THOMAS SALTMARSH , ZHONG XIAOTIAN , MCDONAGH THOMAS E , GEORGIADIS KATY E , SUM PHAIK-ENG , LAVALLIE EDWARD R , COLLINS-RACIE LISA A , CORCORAN CHRISTOPHER JOHN , KUMAR RAVINDRA , HEBERT TRACY , OLLAND STEPHANE HUBERT , MACKIE STEWART ANDREWS
发明人： MOSYAK LIDIA , RUSH III THOMAS SALTMARSH , ZHONG XIAOTIAN , MCDONAGH THOMAS E , GEORGIADIS KATY E , SUM PHAIK-ENG , LAVALLIE EDWARD R , COLLINS-RACIE LISA A , CORCORAN CHRISTOPHER JOHN , KUMAR RAVINDRA , HEBERT TRACY , OLLAND STEPHANE HUBERT , MACKIE STEWART ANDREWS
IPC分类号： C12N9/64 , C30B7/00 , G01N23/20 , G06F19/00
CPC分类号： C12N9/6421 , C07K2299/00 , C30B7/00
摘要： This invention relates to aggrecanase polypeptides and aggrecanase polypeptide/ligand complexes, crystals of aggrecanase and aggrecanase polypeptide/ligand complexes, and related methods and software systems.

8. 发明申请

WO2007025248A2 AGGRECANASE STRUCTURE 审中-公开
标题翻译： AGGRECANASE结构
公开(公告)号：WO2007025248A2
公开(公告)日：2007-03-01
申请号：PCT/US2006033498
申请日：2006-08-24
申请人： WYETH CORP , MOSYAK LIDIA , RUSH III THOMAS SALTMARSH , ZHONG XIAOTIAN , MCDONAGH THOMAS E , GEORGIADIS KATY E , SUM PHAIK-ENG , LAVALLIE EDWARD R , COLLINS-RACIE LISA A , CORCORAN CHRISTOPHER JOHN , KUMAR RAVINDRA , HEBERT TRACY , OLLAND STEPHANE HUBERT , MACKIE STEWART ANDREWS
发明人： MOSYAK LIDIA , RUSH III THOMAS SALTMARSH , ZHONG XIAOTIAN , MCDONAGH THOMAS E , GEORGIADIS KATY E , SUM PHAIK-ENG , LAVALLIE EDWARD R , COLLINS-RACIE LISA A , CORCORAN CHRISTOPHER JOHN , KUMAR RAVINDRA , HEBERT TRACY , OLLAND STEPHANE HUBERT , MACKIE STEWART ANDREWS
IPC分类号： C07K14/43
CPC分类号： C12N9/6421 , C07K2299/00 , C30B7/00
摘要： This invention relates to aggrecanase polypeptides and aggrecanase polypeptide/ligand complexes, crystals of aggrecanase and aggrecanase polypeptide/ligand complexes, and related methods and software systems.
摘要翻译：本发明涉及聚集蛋白聚糖酶多肽和聚集蛋白聚糖酶多肽/配体复合物，聚集蛋白聚糖酶和聚集蛋白聚糖酶多肽/配体复合物的晶体以及相关方法和软件系统。

9. 发明申请

WO2006113861A3 MAMMALIAN EXPRESSION SYSTEMS 审中-公开
公开(公告)号：WO2006113861A3
公开(公告)日：2006-10-26
申请号：PCT/US2006/014859
申请日：2006-04-20
申请人： WYETH , ZHONG, Xiaotian , KRIZ, Ronald , STAHL, Mark
发明人： ZHONG, Xiaotian , KRIZ, Ronald , STAHL, Mark
IPC分类号： C12N5/06 , C07K14/71 , C08B37/10
摘要： The present invention features mammalian expression systems with improved production yields, and method of using these systems to produce desired proteins. In one embodiment, the expression systems of the present invention comprise genetically-engineered mammalian host cells cultured in a medium that contains an effective amount of heparin or heparin-like molecules. The presence of heparin or heparin-like molecules significantly increases protein production by the cultured cells. The present invention also features the use of constitutively-active components of FGFR-I -mediated signal transduction pathways to improve protein production by cultured mammalian cells. Co-expression of such a component with a protein of interest markedly increases the production yield of the protein of interest.

10. 发明申请

WO2007092370A3 LINGO-1 STRUCTURE 审中-公开
标题翻译： LINGO-1结构
公开(公告)号：WO2007092370A3
公开(公告)日：2007-12-13
申请号：PCT/US2007003015
申请日：2007-02-01
申请人： WYETH CORP , MOSYAK LIDIA , DWYER BRIAN , JOHNSON MARK , ZHONG XIAOTIAN , PRESMAN ELEONORA , WILHELM JAMES M , STAHL MARK , SOMERS WILLIAM STUART
发明人： MOSYAK LIDIA , DWYER BRIAN , JOHNSON MARK , ZHONG XIAOTIAN , PRESMAN ELEONORA , WILHELM JAMES M , STAHL MARK , SOMERS WILLIAM STUART
IPC分类号： C07K14/43
CPC分类号： C07K14/4703 , C07K14/705 , C07K2299/00
摘要： This disclosure relates to LINGO-1 polypeptides, LINGO-1 polypeptide/ligand complexes, crystals of LINGO-1 polypeptides, crystals of LINGO-1 polypeptide/ligand complexes, and related methods and software systems.
摘要翻译：本公开涉及LINGO-1多肽，LINGO-1多肽/配体复合物，LINGO-1多肽的晶体，LINGO-1多肽/配体复合物的晶体，以及相关方法和软件系统。

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