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    • 1. 发明申请
    • METHOD FOR PREPARING TRANSFORMED CUCUMIS MELO
    • 用于制备改性CUCUMIS MELO的方法
    • WO2003057890A1
    • 2003-07-17
    • PCT/KR2002/001462
    • 2002-08-01
    • NEXGEN BIOTECHNOLOGIES, INC.LEE, SunYOO, Je-Geun
    • LEE, SunYOO, Je-Geun
    • C12N15/82
    • A01H4/001C12N15/8205
    • The present invention relates to a method of preparing a transformed Cucumis melo using Acrobacterium tumefaciens, more particularly, to a method for preparing a transformed Cucumis melo, which comprises the steps of: (a) inoculating a cotyledon from Cucumis melo with Agrobacterium tumefaciens harboring a suitable vector; (b) regenerating the inoculated cotyledon in a regeneration medium containing 3.0-8.0 mg/l of kinetin as growth regulator and 0.5-3.0 mg/l of IAA (Indole-3-acetic acid) and culturing the inoculated cotyledon to obtain regenerated shoots; and (c) culturing the regenerated shoots on a rooting medium to obtain the transformed Cucumis melo.
    • 本发明涉及使用根癌土壤杆菌制备转化的黄瓜的方法,更具体地说,涉及一种制备转化的黄瓜的方法,该方法包括以下步骤:(a)从含有绿僵菌的根癌土壤杆菌接种来自黄瓜的子叶 合适的载体; (b)在含有3.0-8.0mg / l激动素作为生长调节剂和0.5-3.0mg / l IAA(吲哚-3-乙酸)的再生培养基中再生接种的子叶,并培养接种的子叶以获得再生芽; 和(c)在生根培养基上培养再生芽以获得转化的黄瓜。
    • 5. 发明申请
    • METHOD FOR PREPARING TRANSFORMED CUCUMIS SATIVUS
    • 用于制备转化的CUCUMIS SATIVUS的方法
    • WO2003057889A1
    • 2003-07-17
    • PCT/KR2002/001461
    • 2002-08-01
    • NEXGEN BIOTECHNOLOGIES, INC.LEE, SunYOO, Je-Geun
    • LEE, SunYOO, Je-Geun
    • C12N15/82
    • A01H4/001C12N15/8205
    • The present invention relates to a method of preparing a transformed Cucumis sativus using Acrobacterium tumefaciens, more particularly, to a method for preparing a transformed Cucumis sativus, which comprises the steps of: (a) inoculating a cotyledon from Cucumis sativus with Agrobacterium tumefaciens harboring a suitable vector; (b) placing the inoculated cotyledon on a medium containing BAP (6-benzylaminopurine) and NAA (a-naphthalene acetic acid) and culturing the inoculated cotyledon to obtain regenerated shoots; and (c) culturing the regenerated shoots on a rooting medium to obtain the transformed Cucumis sativus.
    • 本发明涉及使用根癌土壤杆菌制备转化的黄瓜(Cucumis sativus)的方法,更具体地说,涉及一种制备转化的黄瓜的方法,该方法包括以下步骤:(a)从具有根癌土壤杆菌的根癌土壤杆菌接种来自黄瓜的子叶 合适的载体; (b)将接种的子叶放置在含有BAP(6-苄基氨基嘌呤)和NAA(α-萘乙酸)的培养基上,培养接种的子叶以获得再生芽; 和(c)在生根培养基上培养再生芽,以获得转化的黄瓜(Cucumis sativus)。