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    • 7. 发明申请
    • CONTROLLED ADENO-ASSOCIATED VIRUS (AAV) DIVERSIFICATION AND LIBRARIES PREPARED THEREFROM
    • 受控的ADENO相关病毒(AAV)多样性和编写的图书馆
    • WO2011038187A1
    • 2011-03-31
    • PCT/US2010/050135
    • 2010-09-24
    • THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIAWILSON, James, M.VANDENBERGHE, Luc, H.
    • WILSON, James, M.VANDENBERGHE, Luc, H.
    • C12N15/10C12N15/864C12N15/00
    • C12N15/1031C07K14/005C12N2750/14122C12N2750/14143
    • A method for the directed production of a combinatorial library of an altered protein coding sequence is described. The method involves predetermining at least two different nucleotide sequence fragments (regions) which are of interest (ROI) in which the sequence diversity is to be introduced in a manner that may be different for the different domains. The full-length protein coding sequence is divided into predetermined subdomains which are fragments spanning the full-length protein coding sequence. At least one of the subdomains contains the first ROI and at least one of the subdomains contains the second ROI. Primers are designed to specifically amplify these subdomains in a manner which permits the subdomains following treatment as described herein to be directionally and positionally assembled into a combinatorial library of the full-length protein coding sequence which contains altered sequences. Each of the subdomains is amplified with a series of primer sets which comprises at least a first primer set and at least a second primer set, wherein each of the primer sets each consist of a right primer and a left primer which are complementary to a junction located between consecutive fragments in the protein coding sequence. A predetermined subset of the amplified subdomains are subject to different amplification conditions which generate subdomains with altered sequences. Thereafter, the amplified subdomains are pooled and assembled in a directional and positional manner using SOE PCR or other cloning methods to generate a combinatorial library of full-length protein coding sequences which comprise the altered sequences.
    • 描述了用于定向生产改变的蛋白质编码序列的组合文库的方法。 该方法包括预先确定感兴趣的至少两个不同的核苷酸序列片段(区域),其中以对于不同的域可能不同的方式引入序列多样性。 将全长蛋白质编码序列分成预定的子域,其是跨越全长蛋白质编码序列的片段。 至少一个子域包含第一ROI,并且至少一个子域包含第二ROI。 引物设计为以允许如本文所述的治疗后的子结构域定向和定位组装成包含改变的序列的全长蛋白质编码序列的组合文库的方式来特异性地扩增这些亚结构域。 每个子结构域用一系列引物组进行扩增,所述引物组包含至少第一引物组和至少第二引物组,其中每个引物组各自由与引物互补的正确引物和左引物组成 位于蛋白质编码序列的连续片段之间。 经扩增子域的预定子集经受不同的扩增条件,其产生具有改变的序列的亚结构域。 此后,使用SOE PCR或其他克隆方法将扩增的亚结构域以定向和位置方式汇集并组装,以产生包含改变的序列的全长蛋白质编码序列的组合文库。
    • 9. 发明申请
    • CREATION OF A DATA STORE
    • 创建数据存储
    • WO2010091456A1
    • 2010-08-19
    • PCT/AU2010/000134
    • 2010-02-09
    • TAP HOLDINGS PTY LTDLEDWICH, Mark, JosephWILSON, James, Henry
    • LEDWICH, Mark, JosephWILSON, James, Henry
    • G06F7/06G07F17/30
    • G06Q10/10G06Q10/06
    • A method for structuring a data store by analysing source data bases using the steps of relationship discovery, schema merging, hierarchy discovery, heuristic based attribute inclusion and optionally denormalising This is applied to products such as Navision in building an OLAP cube for use in business intelligence applications. Also disclosed is a security adapter to carry security settings from a source data base to an OLAP cube which includes creating a synthetic dimension in the OLAP cube which is a common trait related to all other dimensions in the cube and one role is created for each role in the source data base and users treated as members of those roles as defined in the source data base.
    • 通过使用关系发现,模式合并,层次结构发现,基于启发式的属性包含和任选地非规范化的步骤分析源数据库来构建数据存储的方法。这适用于诸如建筑中的Navision 用于商业智能应用程序的OLAP多维数据集。 还公开了一种安全适配器,用于将源数据库的安全设置传输到OLAP多维数据集,其中包括在OLAP多维数据集中创建一个合成维度,这是与多维数据集中所有其他维度相关的常见特征,并为每个角色创建一个角色 在源数据库和用户视为源数据库中定义的那些角色的成员。