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1. 发明申请

WO2010138679A1 METHOD OF SCREENING DRUGS FOR REVERSAL OF AMYLOID BETA NEUROTOXICITY 审中-公开
标题翻译：筛选用于逆转AMYLOID BET神经毒性的药物的方法
公开(公告)号：WO2010138679A1
公开(公告)日：2010-12-02
申请号：PCT/US2010/036336
申请日：2010-05-27
申请人： UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC. , HICKMAN, James, J. , VARGHESE, Kucku , MOLNAR, Peter
发明人： HICKMAN, James, J. , VARGHESE, Kucku , MOLNAR, Peter
IPC分类号： C12Q1/68
CPC分类号： C07K14/4711
摘要： A method of screening a compound for effectiveness in treating amyloid beta neurotoxicity comprises culturing mammalian neurons in serum-free defined medium until the neurons are electrically functional, exposing the electrically stable neurons to amyloid beta, monitoring the exposed neurons for impairment of electrical functionality, and treating the exposed neurons with the candidate drug while monitoring their electrical activity for reversal of impairment. The invention also includes a method of identifying a mammalian neuron having a biological marker conferring predisposition to development of Alzheimer's disease, the method comprising culturing the mammalian neuron in serum-free medium until the neuron is electrically functional, exposing the electrically stable neuron to amyloid beta while monitoring for impairment of electrical functionality as an indicator of presence of said biological marker, and verifying presence of the biological marker by treating the impaired neuron with an anti-amyloidogenic compound while monitoring for return of neuron functionality.
摘要翻译：筛选化合物以治疗淀粉样蛋白β神经毒性的有效性的方法包括在无血清定义的培养基中培养哺乳动物神经元，直到神经元具有电功能，将电稳定的神经元暴露于淀粉样蛋白β，监测暴露的神经元损害电功能，以及用候选药物治疗暴露的神经元，同时监测其电活动以逆转损伤。本发明还包括鉴定具有赋予阿尔茨海默病发展倾向的生物学标记的哺乳动物神经元的方法，所述方法包括在无血清培养基中培养哺乳动物神经元，直到神经元具有电功能，将电稳定的神经元暴露于淀粉样蛋白β 同时监测电功能损伤作为所述生物标志物存在的指标，以及通过用抗淀粉样变性化合物治疗受损神经元同时监测神经元功能的返回来验证生物标志物的存在。

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