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1. 发明申请

WO2005060345A2 NEW METHOD FOR SEQUENCING-BY-SYNTHESIS 审中-公开
标题翻译：用于顺序合成的新方法
公开(公告)号：WO2005060345A2
公开(公告)日：2005-07-07
申请号：PCT/IB2004/004438
申请日：2004-12-20
申请人： BIOTAGE AB , TOOKE, Nigel , HAGERLID, Peter , EKSTROM, Bjorn
发明人： TOOKE, Nigel , HAGERLID, Peter , EKSTROM, Bjorn
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2565/629 , C12Q2565/301
摘要： The invention refers to a tubular member, such as a pipette tip, for binding nucleic acid molecules for a subsequent biomolecular reaction, to methods for at least partly performing a sequencing-by-synthesis reaction in a tubular member, to an automated system using the tubular member for performing the methods of the invention, and kits and computer programs for use in relation to the methods of the invention. By performing a sequencing-by-synthesis reaction in a tubular member, such as a pipette tip, a new format for enzymatic reactions of this type, i.e. sequencing by synthesis, is provided.
摘要翻译：本发明涉及用于将核酸分子结合用于随后的生物分子反应的管状构件，例如移液管尖端，至少部分地在管状构件中进行逐步合成反应的方法，至使用用于执行本发明的方法的管状构件，以及用于与本发明的方法相关的试剂盒和计算机程序。通过在管状构件（例如移液管尖端）中进行逐步合成反应，提供了这种类型的酶反应的新格式，即通过合成进行的顺序。

2. 发明申请

WO2004029294A1 NEW SEQUENCING METHOD FOR SEQUENCING RNA MOLECULES 审中-公开
标题翻译：用于测序RNA分子的新的测序方法
公开(公告)号：WO2004029294A1
公开(公告)日：2004-04-08
申请号：PCT/SE2003/001499
申请日：2003-09-26
申请人： BIOTAGE , TOOKE, Nigel
发明人： TOOKE, Nigel
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2521/113 , C12Q2565/301
摘要： The present invention provides a method for determination of the identity of at least one nucleotide in a RNA-molecule comprising the steps of: (i) providing the RNA-molecule, an oligonucleotide primer binding to a predetermined position of the RNA molecule, a reverse transcriptase, deoxynucleotides and other necessary reagents, in a reaction vessel; (ii) performing a primer extension reaction, whereby the oligonucleotide primer is extended on the RNA-molecule through incorporation of at least one deoxynucleotide by the action of a reverse transcriptase, resulting in the release of a PPi molecule only upon incorporation of a deoxynucleotide; and (iii) detecting the presence or absence of incorporation, thereby indicating the nucleotide identity of the RNA molecule in the relevant position. In a preferred embodiment, the sequencing of the invention is coupled to the Pyrosequencing TM reaction. A variant of the method employs incorporation of modified nucleotides, with an optionally cleavable linker arm to which is attached a label.
摘要翻译：本发明提供了确定RNA分子中至少一个核苷酸的同一性的方法，包括以下步骤：（i）提供RNA分子，与RNA分子的预定位置结合的寡核苷酸引物，反向转录酶，脱氧核苷酸和其他必需的试剂; （ii）进行引物延伸反应，由此通过逆转录酶的作用通过引入至少一种脱氧核苷酸，在RNA分子上延伸寡核苷酸引物，仅在掺入脱氧核苷酸时导致PPi分子的释放; 和（iii）检测掺入的存在或不存在，从而表明RNA分子在相关位置的核苷酸同一性。在优选的实施方案中，将本发明的测序与焦磷酸测序反应相结合。该方法的变体使用修饰的核苷酸的掺入，以及连接有标记的任选可切割的连接臂。

3. 发明申请

WO2003085135A1 NEW METHOD 审中-公开
标题翻译：新方法
公开(公告)号：WO2003085135A1
公开(公告)日：2003-10-16
申请号：PCT/SE2003/000547
申请日：2003-04-04
申请人： PYROSEQUENCING AB , OLSSON, Charlotta , TOOKE, Nigel
发明人： OLSSON, Charlotta , TOOKE, Nigel
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2563/107 , C12Q2527/137 , C12Q2523/107 , C12Q2533/101 , C12Q2523/119
摘要： The present invention relates to a method for determining the sequence of a nucleic acid molecule comprising the steps of: a) providing a single-stranded form of said nucleic acid molecule; b) hybridizing a primer to said single stranded form of said nucleic acid molecule to form a template/primer complex; c) enzymatically extending the primer by the addition of a polymerase and a mixture of at least one nucleotide and at least one labeled derivative of the at least one nucleotide, wherein the at least one labeled derivative of the at least one nucleotide comprises a label linked to the nucleotide via a cleavable link and wherein the amount of labeled derivative of the at least one nucleotide in said mixture of the at least one nucleotide and the labeled derivative of the at least one nucleotide is within the range of 1-50 mole-%, 1-40 mole-%, 1-30 mole-%, or 1-20 mole-%. d) determining the type of nucleotide added to the primer; and e) repeating steps c) to d) at least once.
摘要翻译：本发明涉及确定核酸分子序列的方法，包括以下步骤：a）提供所述核酸分子的单链形式; b）将引物与所述单链形式的所述核酸分子杂交以形成模板/引物复合物; c）通过加入聚合酶和所述至少一个核苷酸的至少一个核苷酸和至少一个标记的衍生物的混合物酶促延伸引物，其中至少一个核苷酸的至少一个标记的衍生物包含连接的标记通过可切割链接与核苷酸反应，并且其中所述至少一个核苷酸和至少一个核苷酸的标记衍生物的所述混合物中的至少一个核苷酸的标记衍生物的量在1-50摩尔％，1-40摩尔％，1-30摩尔％，或1-20摩尔％。 d）确定添加到引物中的核苷酸类型; 和e）重复步骤c）至d）至少一次。

4. 发明申请

WO2005060345A3 NEW METHOD FOR SEQUENCING-BY-SYNTHESIS 审中-公开
标题翻译：用于顺序合成的新方法
公开(公告)号：WO2005060345A3
公开(公告)日：2006-05-11
申请号：PCT/IB2004004438
申请日：2004-12-20
申请人： BIOTAGE AB , TOOKE NIGEL , HAGERLID PETER , EKSTROM BJORN
发明人： TOOKE NIGEL , HAGERLID PETER , EKSTROM BJORN
IPC分类号： C12Q1/68 , B01D39/16 , B01L3/02
CPC分类号： C12Q1/6869 , C12Q2565/629 , C12Q2565/301
摘要： The invention refers to a tubular member, such as a pipette tip, for binding nucleic acid molecules for a subsequent biomolecular reaction, to methods for at least partly performing a sequencing-by-synthesis reaction in a tubular member, to an automated system using the tubular member for performing the methods of the invention, and kits and computer programs for use in relation to the methods of the invention. By performing a sequencing-by-synthesis reaction in a tubular member, such as a pipette tip, a new format for enzymatic reactions of this type, i.e. sequencing by synthesis, is provided.
摘要翻译：本发明涉及用于将核酸分子结合用于随后的生物分子反应的管状构件，例如移液管尖端，至少部分地在管状构件中进行逐步合成反应的方法，至使用用于执行本发明的方法的管状构件，以及用于与本发明的方法相关的试剂盒和计算机程序。通过在管状构件（例如移液管尖端）中进行逐步合成反应，提供了这种类型的酶反应的新格式，即通过合成进行的顺序。

5. 发明申请

WO2005003386A3 OLIGONUCLEOTIDE LIGATION ASSAY BY DETECTING RELEASED PYROPHOSPHATE 审中-公开
标题翻译：通过检测释放出的磷酸盐来进行寡核苷酸定位测定
公开(公告)号：WO2005003386A3
公开(公告)日：2005-04-14
申请号：PCT/EP2004007090
申请日：2004-06-30
申请人： BIOTAGE AB , TOOKE NIGEL , EKSTROEM BJOERN
发明人： TOOKE NIGEL , EKSTROEM BJOERN
IPC分类号： C12Q20060101 , C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2521/501 , C12Q2565/301
摘要： The present invention is related to a new method for determining the presence of genetic element(s), such as nucleotide repeat(s), or marker(s) for microbial typing, in a nucleic acid sample. The method is based on performing a ligation reaction where after a by-product of the ligation is detected and used to determine the number of nucleotide repeat units in a nucleic acid sample possibly comprising a nucleotide repeat. The invention is also related to kits for performing the method of the present invention and compositions comprising components for performing the present invention.
摘要翻译：本发明涉及用于确定核酸样品中遗传元件（如核苷酸重复）或微生物分型标记物的存在的新方法。该方法基于进行连接反应，其中检测到连接的副产物后，用于确定可能包含核苷酸重复的核酸样品中核苷酸重复单元的数目。本发明还涉及用于实施本发明的方法的试剂盒和包含用于实施本发明的组分的组合物。

6. 发明申请

WO2005003386A2 NEW METHOD 审中-公开
标题翻译：新方法
公开(公告)号：WO2005003386A2
公开(公告)日：2005-01-13
申请号：PCT/EP2004/007090
申请日：2004-06-30
申请人： BIOTAGE AB , TOOKE, Nigel , EKSTRÖM, Björn
发明人： TOOKE, Nigel , EKSTRÖM, Björn
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2521/501 , C12Q2565/301
摘要： The present invention is related to a new method for determining the presence of genetic element(s), such as nucleotide repeat(s), or marker(s) for microbial typing, in a nucleic acid sample. The method is based on performing a ligation reaction where after a by-product of the ligation is detected and used to determine the number of nucleotide repeat units in a nucleic acid sample possibly comprising a nucleotide repeat. The invention is also related to kits for performing the method of the present invention and compositions comprising components for performing the present invention.
摘要翻译：本发明涉及用于确定核酸样品中遗传元件（如核苷酸重复）或微生物分型标记物的存在的新方法。该方法基于进行连接反应，其中检测到连接的副产物后，用于确定可能包含核苷酸重复的核酸样品中核苷酸重复单元的数目。本发明还涉及用于实施本发明的方法的试剂盒和包含用于实施本发明的组分的组合物。

7. 发明申请

WO0147638A3 INTEGRATED MICROFLUIDIC DISC 审中-公开
标题翻译：综合性微流感盘
公开(公告)号：WO0147638A3
公开(公告)日：2002-05-30
申请号：PCT/EP0013014
申请日：2000-12-20
申请人： GYROS AB , TOOKE NIGEL ERIC , ANDERSSON PER
发明人： TOOKE NIGEL ERIC , ANDERSSON PER
IPC分类号： G01N27/447 , B01J19/00 , B01L3/00 , B01L7/00 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N30/88 , G01N35/08 , G01N35/10 , G01N37/00
CPC分类号： B01L3/5027 , B01L7/52 , B01L2200/0631 , B01L2300/0806 , B01L2300/087 , B01L2400/0409 , B01L2400/0421 , B01L2400/088
摘要： Disclosed is a method for performing the steps of nucleic acid template purification, a thermocycling reaction and purification of the products of the thermocycling reaction characterised in that the steps take place sequentially in a microfluidic disc. Also disclosed is a microstructure for fluids comprising at least one inlet opening connected to a first chamber incorporating a means for purifying template nucleic acid which, in turn, is connected to a second chamber incorporating a means for a thermocycling reaction which, in turn, is connected to a third chamber incorporating a means for purifying products of the thermocycling reaction, and a microfluidic disc comprising a plurality of such microstructures.
摘要翻译：公开了一种用于执行核酸模板纯化，热循环反应和热循环反应产物的纯化步骤的方法，其特征在于步骤依次在微流体盘中进行。还公开了一种用于流体的微结构，其包括连接到第一室的至少一个入口开口，该第一室结合有用于纯化模板核酸的装置，该模板核酸又连接到结合有热循环反应装置的第二室，连接到包含用于纯化热循环反应的产物的装置的第三室，以及包括多个这种微结构的微流体盘。

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