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1. 发明申请

WO2003089895A2 METHODS FOR THE ELECTROCHEMICAL DETECTION OF MULTIPLE TARGET COMPOUNDS 审中-公开
标题翻译：电化学检测多种目标化合物的方法
公开(公告)号：WO2003089895A2
公开(公告)日：2003-10-30
申请号：PCT/US2002/029445
申请日：2002-07-17
申请人： UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL , THORP, H., Holden , YANG, Ivana, V. , STEWART, David, H. , GROELKE, John, W. , SZALAI, Veronica, A.
发明人： THORP, H., Holden , YANG, Ivana, V. , STEWART, David, H. , GROELKE, John, W. , SZALAI, Veronica, A.
IPC分类号： G01N
CPC分类号： C12Q1/6825 , C12Q1/004 , C12Q1/005 , C12Q2565/601 , C12Q2563/113 , C12Q2565/607
摘要： A method of detecting two different target molecules through a single electrode is carried out by (a) providing a conductive oxidation-reduction reaction detection electrode; (b) contacting a sample suspected of containing a first and second target molecule to the electrode under conditions in which the first and second target molecules are deposited on the electrode, wherein the first target molecule comprises a first label and the second target molecule comprises a second label; (c) contacting to the electrode a first transition metal complex that oxidizes the first preselected label in a first oxidation-reduction reaction and a second transition metal complex that oxidizes the first and second labels in a second oxidation-reduction reaction, with the first and second oxidation-reduction reactions producing different detectable signals; (d) detecting the presence of the first target molecule by detecting the first oxidation-reduction reaction; and (e) detecting the presence of the second target molecule by detecting the second oxidation-reduction reaction. Devices for carrying out the method are also described.
摘要翻译：通过（a）提供导电氧化还原反应检测电极来进行通过单个电极检测两种不同靶分子的方法; （b）将第一和第二靶分子沉积在电极上的条件下将疑似含有第一和第二靶分子的样品与电极接触，其中第一靶分子包含第一标记，第二靶分子包含第二个标签（c）在第一氧化还原反应中使第一预选标记物氧化的第一过渡金属络合物和在第二氧化还原反应中氧化第一和第二标记的第二过渡金属络合物与第一过渡金属络合物接触，产生不同可检测信号的第二氧化还原反应; （d）通过检测第一氧化还原反应来检测第一靶分子的存在; 和（e）通过检测第二氧化还原反应来检测第二靶分子的存在。还描述了用于执行该方法的装置。

2. 发明申请

WO2003089895A3 METHODS FOR THE ELECTROCHEMICAL DETECTION OF MULTIPLE TARGET COMPOUNDS 审中-公开
公开(公告)号：WO2003089895A3
公开(公告)日：2003-10-30
申请号：PCT/US2002/029445
申请日：2002-07-17
申请人： UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL , THORP, H., Holden , YANG, Ivana, V. , STEWART, David, H. , GROELKE, John, W. , SZALAI, Veronica, A.
发明人： THORP, H., Holden , YANG, Ivana, V. , STEWART, David, H. , GROELKE, John, W. , SZALAI, Veronica, A.
IPC分类号： C12Q1/68
摘要： A method of detecting two different target molecules through a single electrode is carried out by (a) providing a conductive oxidation-reduction reaction detection electrode; (b) contacting a sample suspected of containing a first and second target molecule to the electrode under conditions in which the first and second target molecules are deposited on the electrode, wherein the first target molecule comprises a first label and the second target molecule comprises a second label; (c) contacting to the electrode a first transition metal complex that oxidizes the first preselected label in a first oxidation-reduction reaction and a second transition metal complex that oxidizes the first and second labels in a second oxidation-reduction reaction, with the first and second oxidation-reduction reactions producing different detectable signals; (d) detecting the presence of the first target molecule by detecting the first oxidation-reduction reaction; and (e) detecting the presence of the second target molecule by detecting the second oxidation-reduction reaction. Devices for carrying out the method are also described.

3. 发明申请

WO2008048216A3 ELECTROCATALYTIC DETECTION OF CARBON NANOTUBES 审中-公开
标题翻译：碳纳米管的电催化检测
公开(公告)号：WO2008048216A3
公开(公告)日：2008-09-12
申请号：PCT/US2006029030
申请日：2006-07-26
申请人： UNIV NORTH CAROLINA , THORP H HOLDEN , NAPIER MARY E
发明人： THORP H HOLDEN , NAPIER MARY E
IPC分类号： G01N19/08
CPC分类号： C12Q1/6813
摘要： A method of detecting a carbon nanotube, is carried out by: (a) reacting the carbon nanotube with a first transition metal complex that oxidizes the carbon nanotube in a first oxidation-reduction reaction, regenerating the reduced form of the first transition metal complex in a catalytic cycle; and (b) detecting the carbon nanotube by detecting the first oxidation-reduction reaction. The method may be used to detect a target compound (e.g., a protein, peptide or nucleic acid) directly or indirectly coupled to the carbon nanotube. Suitable transition metal complexes include but are not limited to Ru, Fe, Os or Re complexes.
摘要翻译：检测碳纳米管的方法通过以下方式进行：（a）使碳纳米管与第一过渡金属配合物反应，所述第一过渡金属配合物在第一氧化 - 还原反应中氧化碳纳米管，将还原形式的第一过渡金属配合物在催化循环; 和（b）通过检测第一次氧化还原反应来检测碳纳米管。该方法可用于检测直接或间接偶联至碳纳米管的目标化合物（例如，蛋白质，肽或核酸）。合适的过渡金属络合物包括但不限于Ru，Fe，Os或Re络合物。

4. 发明申请

WO2008030252A3 COMPOSITIONS AND METHODS OF SCREENING APOPROTEINS 审中-公开
标题翻译：组合物和筛选APOPROTEIN的方法
公开(公告)号：WO2008030252A3
公开(公告)日：2008-06-05
申请号：PCT/US2006041221
申请日：2006-10-20
申请人： VIAMET PHARMACEUTICALS INC , THORP H HOLDEN , O'HALLORAN THOMAS V
发明人： THORP H HOLDEN , O'HALLORAN THOMAS V
IPC分类号： G01N33/68 , C12Q1/00
CPC分类号： G01N33/6803 , C12Q1/00 , G01N2500/04
摘要： The present invention is directed to compositions and methods of screening apoproteins for the elucidation of modulators of metalation of apoproteins.
摘要翻译：本发明涉及筛选载脂蛋白的组合物和方法，以阐明脱辅基蛋白的金属化调节剂。

5. 发明申请

WO2008030252A2 COMPOSITIONS AND METHODS OF SCREENING APOPROTEINS 审中-公开
标题翻译：筛选APOPROTEINS的组合物和方法
公开(公告)号：WO2008030252A2
公开(公告)日：2008-03-13
申请号：PCT/US2006/041221
申请日：2006-10-20
申请人： VIAMET PHARMACEUTICALS, INC. , THORP, H., Holden , O'HALLORAN, Thomas, V.
发明人： THORP, H., Holden , O'HALLORAN, Thomas, V.
IPC分类号： G01N33/50
CPC分类号： G01N33/6803 , C12Q1/00 , G01N2500/04
摘要： The present invention is directed to compositions and methods of screening apoproteins for the elucidation of modulators of metalation of apoproteins.
摘要翻译：本发明涉及用于阐明载脂蛋白金属化调节剂的载脂蛋白筛选组合物和方法。

6. 发明申请

WO0222711A3 COMPOSITIONS OF NUCLEIC ACIDS THAT FORM MOLTEN PHASES AND METHODS OF USE THEREOF 审中-公开
标题翻译：形成固定相的核酸组成及其使用方法
公开(公告)号：WO0222711A3
公开(公告)日：2003-01-16
申请号：PCT/US0125718
申请日：2001-08-17
申请人： UNIV NORTH CAROLINA , THORP H HOLDEN , MURRAY ROYCE W , LEONE ANTHONY M , WILLIAMS MARY ELIZABETH
发明人： THORP H HOLDEN , MURRAY ROYCE W , LEONE ANTHONY M , WILLIAMS MARY ELIZABETH
IPC分类号： C08G65/329 , C08G65/333 , C08G81/00 , C08L101/02 , C09D201/02 , G11C13/02
CPC分类号： G11C13/0019 , B82Y10/00 , C08G65/329 , C08G65/333 , C08G81/00 , C08L101/02 , C09D201/02 , G11C13/0014 , Y10T428/31504 , Y10T428/31663 , Y10T428/31678 , Y10T428/31692 , Y10T428/31855 , Y10T428/31971
摘要： Compositions of biomolecules such as nucleic acids that form molten salts are provided. These compositions molten compositions that have useful electrical properties. Such compositions include a salt of (i) an organic polymer ion such as a polynucleic acid anion, and (ii) a polyether or polysiloxane couterion. Methods of making and using such compositions, along with electrical devices such as memory devices, are also provided.
摘要翻译：提供生物分子的组成，例如形成熔融盐的核酸。这些组合物具有有用的电性能的熔融组合物。这样的组合物包括（i）有机聚合物离子如多核酸阴离子的盐，和（ii）聚醚或聚硅氧烷。还提供了制造和使用这些组合物的方法，以及诸如记忆装置的电气装置。

7. 发明申请

WO0242771A3 DETECTION OF BINDING REACTIONS USING CATALYTIC ELECTROCHEMICAL LABELS 审中-公开
标题翻译：使用催化电化学标记检测结合反应
公开(公告)号：WO0242771A3
公开(公告)日：2002-09-12
申请号：PCT/US0121571
申请日：2001-07-09
申请人： UNIV NORTH CAROLINA , XANTHON INC , STEWART DAVID H , GROELKE JOHN W , THORP H HOLDEN , ECKHARDT ALLEN E
发明人： STEWART DAVID H , GROELKE JOHN W , THORP H HOLDEN , ECKHARDT ALLEN E
IPC分类号： A61B5/05 , C07H21/02 , C12M1/34 , C12P19/34 , C12Q1/68 , G01N27/26 , G01N33/48 , G01N33/50 , G01N33/53 , G01N33/542 , G01N33/543 , G06F13/42 , G06F19/00 , G01N27/327 , G01N33/58
CPC分类号： B82Y30/00 , B82Y5/00 , C12Q1/6825 , G01N33/5438 , C12Q2565/607 , C12Q2563/137 , C12Q2563/113
摘要： A method of detecting binding interactions and target molecules, such as proteins, protein fragments, recombinant proteins, recombinant protein fragments, extracellular matrix proteins, ligands, carbohydrates, steroids, hormones, drugs, drug candidates, immunoglobulins and receptors of eukaryotic, prokaryotic or viral origin, by mediated electrochemistry using labels that react with transition metal mediator complexes in a detectable catalytic redox reaction. These labels are attached directly to binders, target molecules, surrogate target molecules, or to affinity ligands capable of binding to the target or to surrogate target molecules capable of competing with the target for binding to another binder. The labels can be naturally present (endogenous) in the binder, target or affinity ligand, or constructed by the covalent attachment of the label to the binder, target, affinity ligand or surrogate target (exogenous).
摘要翻译：检测结合相互作用和靶分子的方法，例如蛋白质，蛋白质片段，重组蛋白，重组蛋白片段，细胞外基质蛋白，配体，碳水化合物，类固醇，激素，药物，候选药物，免疫球蛋白和真核，原核或病毒的受体起源，通过介导的电化学使用在可检测的催化氧化还原反应中与过渡金属介体复合物反应的标记。这些标记物直接连接到粘合剂，靶分子，替代靶分子，或与能够结合靶的亲和配体或替代能与靶标竞争结合另一种粘合剂的靶分子。标签可以在粘合剂，靶或亲和配体中天然存在（内源的），或者通过标记共价连接到粘合剂，靶，亲和配体或替代靶（外源的）上构建。

8. 发明申请

WO9835232A3 ELECTROCHEMICAL PROBES FOR DETECTION OF MOLECULAR INTERACTIONS AND DRUG DISCOVERY 审中-公开
标题翻译：用于检测分子相互作用和药物发现的电化学探针
公开(公告)号：WO9835232A3
公开(公告)日：1999-03-04
申请号：PCT/US9802440
申请日：1998-02-06
申请人： UNIV NORTH CAROLINA , NOVALON PHARMACEUTICAL CORP , FOWLKES DANA M , THORP H HOLDEN
发明人： FOWLKES DANA M , THORP H HOLDEN
IPC分类号： G01N33/483 , C12Q1/00 , G01N27/327 , G01N33/543 , G01N33/53
CPC分类号： B82Y30/00 , C12Q1/004 , G01N33/5438 , G01N2333/02 , G01N2333/162 , G01N2333/36 , G01N2333/42 , G01N2333/4727 , G01N2333/503 , G01N2333/70503 , G01N2333/70546 , G01N2333/7056 , G01N2333/71 , G01N2333/715 , G01N2333/745 , G01N2333/78 , G01N2333/8107 , G01N2333/9726
摘要： This invention relates to methods and apparatus for performing electrochemical analyses. The invention provides an electrochemical apparatus for performing potentiometric analyses for detecting specific binding between a first member of a biological binding pair immobilized on an electrode and a second member of a biological binding pair that is electrochemically labeled, in the presence of an electrochemical mediator. Methods for using the apparatus of the invention for performing binding and competition binding assays are provided. The invention also provides methods for performing high throughput screening assays for detecting inhibition of specific binding between the members of the biological binding pair for use in drug development, biochemical analysis and protein purification assays.

9. 发明申请

WO1997001646A2 ELECTROCHEMICAL DETECTION OF NUCLEIC ACID HYBRIDIZATION 审中-公开
标题翻译：电化学检测核酸杂交
公开(公告)号：WO1997001646A2
公开(公告)日：1997-01-16
申请号：PCT/US1996010702
申请日：1996-06-24
申请人： THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL , THORP, H., Holden , JOHNSTON, Dean, H.
发明人： THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
IPC分类号： C12Q01/68
CPC分类号： B82Y30/00 , B82Y15/00 , C12Q1/6816 , C12Q1/6825 , C12Q2525/197
摘要： A method of detecting a nucleic acid (e.g., DNA, RNA) that contains at least one preselected base (e.g., adenine, guanine, 6-mercaptoguanine, 8-oxo-guanine, and 8-oxo-adenine) comprises: (a) reacting the nucleic acid with a transition metal complex capable of oxidizing the preselected base in an oxidation-reduction reaction; (b) detecting the oxidation-reduction reaction; and (c) determining the presence or absence of the nucleic acid from the detected oxidation-reduction reaction at the preselected base. The method may be used in a variety of applications, including DNA sequencing, diagnostic assays, and quantitative analysis.
摘要翻译：检测含有至少一个预选碱基（例如腺嘌呤，鸟嘌呤，6-巯基鸟嘌呤，8-氧代鸟嘌呤和8-氧代腺嘌呤）的核酸（例如DNA，RNA）的方法包括：（a）使核酸与能在氧化 - 还原反应中氧化预选碱的过渡金属配合物反应; （b）检测氧化还原反应; 和（c）从预选碱基处检测到的氧化 - 还原反应确定核酸的存在或不存在。该方法可用于多种应用，包括DNA测序，诊断测定和定量分析。

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