专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO0220836A2 METHOD OF SEQUENCING DNA 审中-公开
标题翻译：序列DNA的方法
公开(公告)号：WO0220836A2
公开(公告)日：2002-03-14
申请号：PCT/GB0104015
申请日：2001-09-07
申请人： PYROSEQUENCING AB , GARDNER REBECCA , NYREN PAAL , RONAGHI MOSTAFA , TALLSJOE ANNIKA
发明人： NYREN PAAL , RONAGHI MOSTAFA , TALLSJOE ANNIKA
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2565/301
摘要： The present invention provides a method of identifying a base at a target position in a sample nucleic acid sequence, said method comprising: subjecting a primer hybridised to said sample nucleic acid immediately adjacent to the target position, to a polymerase primer extension reaction in the presence of a nucleotide, whereby the nucleotide will only become incorporated if it is complementary to the base in the target position, and determining whether or not said nucleotide is incorporated by detecting whether Ppi is released, the identity of the target base being determined from the identity of any nucleotide incorporated, wherein, where said nucleotide comprises an adenine base, an alpha -thio triphosphate analogue of said nucleotide is used, ant the Rp isomer of said analogue and/or the degradation products of said analogue are eliminated from the polymerase reaction step.
摘要翻译：本发明提供了鉴定样品核酸序列中目标位置的碱基的方法，所述方法包括：使与所述目标位置相邻的所述样品核酸杂交的引物在存在的情况下进行聚合酶引物延伸反应的核苷酸，其核苷酸仅在与目标位置的碱基互补时才被引入，并且通过检测Ppi是否被释放来确定所述核苷酸是否被并入，靶基因的身份是根据身份确定的其中，当所述核苷酸包含腺嘌呤碱基时，使用所述核苷酸的α-硫代三磷酸类似物，所述类似物的Rp异构体和/或所述类似物的降解产物的蚂蚁从聚合酶反应步骤中消除。

IPRDB

热门服务

关于我们

友情链接

联系方式