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    • 6. 发明申请
    • MICROARRAY-BASED SINGLE NUCLEOTIDE POLYMORPHISM, SEQUENCING, AND GENE EXPRESSION ASSAY METHOD
    • 基于微阵列的单核苷酸多态性,序列和基因表达测定方法
    • WO2006113931A2
    • 2006-10-26
    • PCT/US2006/015323
    • 2006-04-20
    • COMBIMATRIX CORPORATIONLODES, Michael, J.SUCIU, DominicMcSHEA, Andrew
    • LODES, Michael, J.SUCIU, DominicMcSHEA, Andrew
    • C12Q1/68C12P19/34
    • C12Q1/6874
    • There is disclosed a microarray-based single nucleotide polymorphism, sequencing, and gene expression assay method. Specifically, there is disclosed a method using a microarray device wherein a plurality of hybridized structures is formed by contacting the microarray under a hybridizing condition to a hybridizing solution comprising a plurality of tagged targets and a plurality of detection sequences. The detection sequences of each hybridized structure is extended using an extension-ligation solution and an extension-ligation condition. After extension, ligation of the extended sequence occurs to a probe if the terminal nucleotide of a probe is complementary to the hybridized tagged targets. Non-bound material is removed by using a washing solution and a washing method. The target nucleotide and the target sequence of the tagged targets is determined by which probe is ligated to the detection sequences.
    • 公开了基于微阵列的单核苷酸多态性,测序和基因表达测定方法。 具体地,公开了使用微阵列装置的方法,其中通过在杂交条件下将微阵列与包含多个标记的靶和多个检测序列的杂交溶液接触而形成多个杂交结构。 使用延伸连接溶液和延伸连接条件扩展每个杂交结构的检测序列。 延伸后,如果探针的末端核苷酸与杂交标记的靶标互补,则延伸序列的连接发生在探针上。 通过使用洗涤溶液和洗涤方法除去未结合的物质。 通过将哪个探针连接到检测序列来确定标记的靶标的靶核苷酸和靶序列。