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1. 发明申请

WO2012138973A3 COMPOSITION AND METHODS RELATED TO MODIFICATION OF 5-METHYLCYTOSINE (5mC) 审中-公开
公开(公告)号：WO2012138973A3
公开(公告)日：2012-10-11
申请号：PCT/US2012/032489
申请日：2012-04-06
申请人： THE UNIVERSITY OF CHICAGO , HE, Chuan , ZHANG, Liang , SONG, Chunxiao , YU, Miao
发明人： HE, Chuan , ZHANG, Liang , SONG, Chunxiao , YU, Miao
IPC分类号： C12Q1/68
摘要： The present invention relates to methods and compositions for detecting, evaluating, and/or mapping 5 methyl-modified and/or 5-hydroxylmethyl-modified cytosine bases within a nucleic acid molecule. In certain aspects the methods further comprise modifying 5hmC with a first detectable label or a first detectable functional group. In certain aspects a converted or modified 5-methylcytosine is labeled with a second detectable label. The method can also include a step of detecting differential labeling of the nucleic acid with the first and second label. The label or detectable label can be a fluorescent radioactive, enzymatic, electrochemical, or colorimetric label. In certain aspects the hydroxyl group of a modified 5mC is further converted to a functional group selected from an aldehyde or carboxyl group, which may in turn be coupled to a label or detectable label. The functional group, e.g., hydroxyl group, of modified 5mC can be further modified using an enzymatic method, such as modification by alcohol dehydrogenase. The functional group, e.g., hydroxyl group, of modified 5mC can be further modified using a chemical method, such as modification by pyridinium chlorochromate (PCC). In certain aspects 5hmC, endogenous and/or modified, is glucosylated. The glucosylated 5hmC (5gmC) can comprise a first, second, and or third label, or more. In certain aspects the label is fluorescent, radioactive, enzymatic, electrochemical, or colorimetric.

2. 发明申请

WO2011127136A1 COMPOSITION AND METHODS RELATED TO MODIFICATION OF 5-HYDROXYMETHYLCYTOSINE (5-HMC) 审中-公开
标题翻译：与5-羟基甲基吗啉（5-HMC）的改性相关的组合物和方法
公开(公告)号：WO2011127136A1
公开(公告)日：2011-10-13
申请号：PCT/US2011/031370
申请日：2011-04-06
申请人： UNIVERSITY OF CHICAGO , HE, Chuan , SONG, Chunxiao
发明人： HE, Chuan , SONG, Chunxiao
IPC分类号： C12P19/18 , C12N9/10
CPC分类号： C12Q1/6883 , C12P19/34 , C12Q1/48 , C12Q1/6827 , C12Q2600/154 , C12Y204/01028 , C12Q2521/531 , C12Q2537/164
摘要： The present invention relates generally to the field of molecular biology. More particularly, it concerns methods and compositions for detecting, evaluating, and/or mapping 5 -hydro xym ethyl -modified cytosine bases within a nucleic acid molecule.
摘要翻译：本发明一般涉及分子生物学领域。更具体地说，本发明涉及用于检测，评估和/或测定核酸分子内5-羟色胺乙基修饰的胞嘧啶碱基的方法和组合物。

3. 发明申请

WO2012138973A2 COMPOSITION AND METHODS RELATED TO MODIFICATION OF 5-METHYLCYTOSINE (5mC) 审中-公开
标题翻译：与5-甲基喹啉（5mC）相关的组合物和方法
公开(公告)号：WO2012138973A2
公开(公告)日：2012-10-11
申请号：PCT/US2012032489
申请日：2012-04-06
申请人： UNIV CHICAGO , HE CHUAN , ZHANG LIANG , SONG CHUNXIAO , YU MIAO
发明人： HE CHUAN , ZHANG LIANG , SONG CHUNXIAO , YU MIAO
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6827 , C12Q2521/531 , C12Q2537/164
摘要： The present invention relates to methods and compositions for detecting, evaluating, and/or mapping 5 -methyl-modified and/or 5-hydroxylmefhyl-modified cytosine bases within a nucleic acid molecule.
摘要翻译：本发明涉及用于检测，评估和/或测定核酸分子内的5-甲基修饰和/或5-羟甲基修饰的胞嘧啶碱基的方法和组合物。

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