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1. 发明申请

WO2012028709A3 NOVEL MONOOXYGENASE VARIANTS 审中-公开
标题翻译：新的MONOOXYGENASE变体
公开(公告)号：WO2012028709A3
公开(公告)日：2012-04-26
申请号：PCT/EP2011065179
申请日：2011-09-02
申请人： BRAIN BIOTECHNOLOGY RES & INFORMATION NETWORK AG , SCHWANEBERG ULRICH , BLANUSA MILAN , NIEHAUS FRANK , ECK JUERGEN
发明人： SCHWANEBERG ULRICH , BLANUSA MILAN , NIEHAUS FRANK , ECK JUERGEN
IPC分类号： C12N9/02
CPC分类号： C12Y106/02004 , C12N9/0042
摘要： The present invention relates to a nucleic acid molecule encoding a polypeptide having cytochrome P450 monooxygenase activity, wherein said polypeptide comprises a reductase domain that deviates by at least one mutation from (a) the reductase domain of cytochrome P450 BM3, wherein the reductase domain of cytochrome P450 BM3 is represented by SEQ ID NO: 1; or (b) a reductase domain having at least 95% sequence identity to SEQ ID NO:1; and wherein said mutation(s) result(s) in an increased cytochrome P450 monooxygenase activity as compared to a polypeptide comprising the reductase domain of SEQ ID NO: 1. The present invention also relates to a vector comprising the nucleic acid molecule of the invention and a host transformed with the vector. Furthermore, the invention relates to a method of producing a polypeptide comprising culturing the host of the invention as well as to a polypeptide encoded by the nucleic acid molecule of the invention or produced by the method of the invention. The present invention further relates to the use of the polypeptide of the invention in biotransformation or fine chemical synthesis, to an oligo- or polynucleotide which specifically hybridizes to the nucleic acid molecule of the invention as well as to a composition and to a kit.
摘要翻译：本发明涉及编码具有细胞色素P450单加氧酶活性的多肽的核酸分子，其中所述多肽包含与（a）细胞色素P450 BM3的还原酶结构域至少一个突变偏差的还原酶结构域，其中细胞色素的还原酶结构域 P450 BM3由SEQ ID NO：1表示; 或（b）与SEQ ID NO：1具有至少95％序列同一性的还原酶结构域; 并且其中与包含SEQ ID NO：1的还原酶结构域的多肽相比，所述突变导致细胞色素P450单加氧酶活性增加。本发明还涉及包含本发明的核酸分子的载体和用载体转化的宿主。此外，本发明涉及生产多肽的方法，其包括培养本发明的宿主以及由本发明的核酸分子编码的多肽或通过本发明的方法产生的多肽。本发明进一步涉及本发明的多肽在生物转化或精细化学合成中的用途，涉及与本发明的核酸分子以及组合物和试剂盒特异性杂交的寡核苷酸或多核苷酸。

2. 发明申请

WO2003008563A2 IMPROVED CYTOCHROME P450 OXYGENASES 审中-公开
标题翻译：改进的CYTOCHROME P450氧化剂
公开(公告)号：WO2003008563A2
公开(公告)日：2003-01-30
申请号：PCT/US2002/023340
申请日：2002-07-22
申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY , FARINAS, Edgardo, T. , GLIEDER, Anton , ARNOLD, Frances, H. , SCHWANEBERG, Ulrich
发明人： FARINAS, Edgardo, T. , GLIEDER, Anton , ARNOLD, Frances, H. , SCHWANEBERG, Ulrich
IPC分类号： C12N
CPC分类号： C12N9/0071 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , Y02E50/10 , Y02P20/52
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
摘要翻译：提供了编码细胞色素P450变体的核酸。细胞色素P450变体具有比相应的野生型或亲本细胞色素P450酶更高的烷烃氧化能力，烯烃氧化能力和/或较高的有机溶剂耐受性。优选的野生型细胞色素P450是细胞色素P450 BM-3。优选的细胞色素P450变体包括具有改善含有少于8个碳原子的烷烃和环氧化烯烃羟基化能力以及对应于细胞色素P450 BM-3的V78A，H236Q和E252G的氨基酸取代的那些变体。优选的细胞色素P450变体还包括在包含共溶剂如DMSO和THF的溶液中具有改善的羟基化活性的那些，并且具有对应于细胞色素P450 BM-3的T235A，R471A，E494K和S1024E的氨基酸取代。

3. 发明申请

WO2012017008A1 A NOVEL GLUCOSE OXIDASE 审中-公开
标题翻译：新型葡萄糖氧化酶
公开(公告)号：WO2012017008A1
公开(公告)日：2012-02-09
申请号：PCT/EP2011/063368
申请日：2011-08-03
申请人： B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG , SCHWANEBERG, Ulrich , PRODANOVIC, Radivoje , OSTAFE, Raluca , NIEHAUS, Frank , ECK, Jürgen
发明人： SCHWANEBERG, Ulrich , PRODANOVIC, Radivoje , OSTAFE, Raluca , NIEHAUS, Frank , ECK, Jürgen
IPC分类号： C12N9/04
CPC分类号： C12N9/0006
摘要： The present invention relates to a novel nucleic acid molecule encoding (I) a polypeptide having the activity of a glucose oxidase (EC 1.1.3.4); or (II) a fragment of the polypeptide of (I) having the activity of a glucose oxidase and comprising at least 400 amino acids. Moreover, the invention relates to a vector encoding the nucleic acid molecule, a host cell transformed, transduced or transfected with the vector, a the protein encoded by the nucleic acid molecule, and compositions comprising said nucleic acid molecule, vector, host cell, protein or combinations thereof.
摘要翻译：本发明涉及编码（I）具有葡萄糖氧化酶活性的多肽的新型核酸分子（EC 1.1.3.4）; 或（II）具有葡萄糖氧化酶活性且包含至少400个氨基酸的（I）多肽的片段。此外，本发明涉及编码核酸分子的载体，用载体转化，转导或转染的宿主细胞，由核酸分子编码的蛋白质，以及包含所述核酸分子，载体，宿主细胞，蛋白质的组合物或其组合。

4. 发明申请

WO2011110625A1 LEISTUNGSVERBESSERTE PROTEASEVARIANTE 审中-公开
标题翻译：性能增强的蛋白酶变体
公开(公告)号：WO2011110625A1
公开(公告)日：2011-09-15
申请号：PCT/EP2011/053607
申请日：2011-03-10
申请人： HENKEL AG & CO. KGAA , SIEGERT, Petra , SCHWANEBERG, Ulrich , MARTINEZ MOYA, Ronny , MERKEL, Marion , SPITZ, Astrid , WIELAND, Susanne , HELLMUTH, Hendrik , MAURER, Karl-Heinz
发明人： SIEGERT, Petra , SCHWANEBERG, Ulrich , MARTINEZ MOYA, Ronny , MERKEL, Marion , SPITZ, Astrid , WIELAND, Susanne , HELLMUTH, Hendrik , MAURER, Karl-Heinz
IPC分类号： C12N9/54
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681
摘要： Proteasen umfassend eine Aminosäuresequenz, die zu der in SEQ ID NO.1 angegebenen Aminosäuresequenz über deren Gesamtlänge zu mindestens 70% identisch sind und in der Zählung gemäß SEQ ID NO.1 die Aminosäuresubstitution 121V aufweisen, bzw. Mittel, die solche Proteasen umfassen, zeigen eine sehr gute Reinigungsleistung an protease-sensitiven Anschmutzungen.
摘要翻译：蛋白酶，其包含氨基酸序列，其是相同的在其整个长度中SEQ ID NO.1的氨基酸序列所示的序列和具有至少70％，根据根据SEQ ID NO.1，氨基酸取代121V，或包含这样的蛋白酶显示出组合物的编号蛋白酶敏感的污渍有很好的清洁性能。

5. 发明申请

WO2008000738A1 PROTEINE MIT ESTERASE-AKTIVITÄT 审中-公开
标题翻译：与酯酶活性蛋白质
公开(公告)号：WO2008000738A1
公开(公告)日：2008-01-03
申请号：PCT/EP2007/056367
申请日：2007-06-26
申请人： BASF AKTIENGESELLSCHAFT , BREUER, Michael , HAUER, Bernhard , SCHWANEBERG, Ulrich , WONG, Tuck, Seng
发明人： BREUER, Michael , HAUER, Bernhard , SCHWANEBERG, Ulrich , WONG, Tuck, Seng
IPC分类号： C12N9/16 , C12P41/00
CPC分类号： C12N9/16 , C12P7/62 , C12P41/003
摘要： Die Erfindung betrifft neuartige Proteine mit Esterase-Aktivität und Mutanten davon, dafür kodierende Nukleinsäuresequenzen, Expressionskassetten, Vektoren und rekombinante Mikroorganismen; Verfahren zur Herstellung dieser Proteine und deren Verwendung zur enzymatischen, insbesondere enantioselektiven enzymatischen Esterhydrolyse oder Umesterung organischer Ester.
摘要翻译：本发明涉及具有酯酶活性的和突变体，用于编码的核酸序列，表达盒，载体和重组微生物的新蛋白质; 一种用于生产这些蛋白质的以及它们用于酶的使用，映体选择性酶酯水解或酯交换特别是有机酯的方法。

6. 发明申请

WO2013037609A3 METHOD FOR ADAPTING A HYDROLYTIC ENZYME TO A COMPONENT THAT STABILIZES THE HYDROLYTIC ENZYME 审中-公开
标题翻译：将水解酶适配到水解酶稳定化组分的方法
公开(公告)号：WO2013037609A3
公开(公告)日：2013-05-30
申请号：PCT/EP2012066237
申请日：2012-08-21
申请人： HENKEL AG & CO KGAA , SIEGERT PETRA , EVERS STEFAN , MERKEL MARION , MUSMANN NINA , HELLMUTH HENDRIK , O'CONNELL TIMOTHY , MAURER KARL-HEINZ , SCHWANEBERG ULRICH , JAKOB FELIX , MARTINEZ RONNY , LAUFS BRIAN , AYDEMIR AYHAN , WEBER THOMAS
发明人： SIEGERT PETRA , EVERS STEFAN , MERKEL MARION , MUSMANN NINA , HELLMUTH HENDRIK , O'CONNELL TIMOTHY , MAURER KARL-HEINZ , SCHWANEBERG ULRICH , JAKOB FELIX , MARTINEZ RONNY , LAUFS BRIAN , AYDEMIR AYHAN , WEBER THOMAS
IPC分类号： C11D3/386 , C12N9/54
CPC分类号： C12Q1/37 , C11D3/38663 , C11D3/38681 , C12N9/54 , C12N9/96
摘要： The stabilization of a hydrolytic enzyme in a liquid preparation is to be improved through a component that stabilizes the hydrolytic enzyme. This is accomplished by a method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme, comprising the following method steps: a) providing a hydrolytic enzyme (starting enzyme) and a component that stabilizes the hydrolytic enzyme and that comprises a reversible inhibitor of the hydrolytic enzyme; b) changing the amino acid sequence of the hydrolytic enzyme in at least one position, by substitution, deletion or insertion; c) determining the relative activity of the hydrolytic enzyme from step b) and the relative activity of the starting enzyme in a liquid preparation; d) selecting the hydrolytic enzyme that has a reduced relative activity by comparison with the relative activity of the starting enzyme.
摘要翻译：液态制剂中水解酶的稳定化可通过水解酶稳定组分来改善。这是通过一种用于适配水解酶来水解酶稳定化组分，其包括以下步骤实现：a）提供水解酶（亲本酶）和包含水解酶的可逆抑制剂的水解酶稳定组分; b）通过取代，缺失或插入在至少一个位置改变水解酶的氨基酸序列; c）测定步骤b）的水解酶的相对活性和起始酶在液体制剂中的相对活性; d）选择与亲本酶的相对活性相比具有降低的相对活性的水解酶。

7. 发明申请

WO2010070086A3 VERFAHREN ZUR ENZYMATISCHEN UMSETZUNG VON ALKANEN 审中-公开
公开(公告)号：WO2010070086A3
公开(公告)日：2010-06-24
申请号：PCT/EP2009/067493
申请日：2009-12-18
申请人： EVONIK DEGUSSA GMBH , GRÖGER, Harald , BURDA, Edyta , SCHWANEBERG, Ulrich , MARIENHAGEN, Jan , DRAUZ, Karlheinz
发明人： GRÖGER, Harald , BURDA, Edyta , SCHWANEBERG, Ulrich , MARIENHAGEN, Jan , DRAUZ, Karlheinz
IPC分类号： C12P7/02 , C12P7/26 , C12N9/02 , C12N9/04 , C12N15/53
摘要： Die Erfindung betrifft ein Verfahren zur Herstellung von Ketonen durch enzymatische Oxidation von Alkanen, bei dem man diese Alkane in Gegenwart einer Monooxygenase, einer Alkoholdehydrogenase und eines für beide Enzyme geeigneten Cofaktors in einem wässrigen Reaktionsmedium in Gegenwart von molekularem Sauerstoff umsetzt.

8. 发明申请

WO2005073402A1 VESIKEL ZUM ABTRENNEN VON SUBSTANZEN AUS FLÜSSIGEN MEDIEN 审中-公开
标题翻译：囊泡用于分离物质从液体介质
公开(公告)号：WO2005073402A1
公开(公告)日：2005-08-11
申请号：PCT/EP2005/001039
申请日：2005-02-02
申请人： INTERNATIONAL UNIVERSITY BREMEN GMBH , UNIVERSITÄT BASEL , SCHWANEBERG, Ulrich , WINTERHALTER, Mathias , MEIER, Wolfgang
发明人： SCHWANEBERG, Ulrich , WINTERHALTER, Mathias , MEIER, Wolfgang
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12Q2563/119 , C12Q2563/161
摘要： Die Erfindung betrifft Verfahren zum Abtrennen von Substanzen aus flüssigen Medien und dazu geeignete Trennmittel.
摘要翻译：本发明涉及一种用于从液体培养基加上一个合适的脱模剂分离物质的方法。

9. 发明申请

WO2003008563A3 IMPROVED CYTOCHROME P450 OXYGENASES 审中-公开
公开(公告)号：WO2003008563A3
公开(公告)日：2003-01-30
申请号：PCT/US2002/023340
申请日：2002-07-22
申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY , FARINAS, Edgardo, T. , GLIEDER, Anton , ARNOLD, Frances, H. , SCHWANEBERG, Ulrich
发明人： FARINAS, Edgardo, T. , GLIEDER, Anton , ARNOLD, Frances, H. , SCHWANEBERG, Ulrich
IPC分类号： C12N9/02 , C12N1/20 , C12N15/00 , C12Q1/00 , C12Q1/34 , C12Q1/68 , C12P21/06 , C12P21/04 , A61K38/44 , C07H21/04 , C07K1/00 , C07K16/00
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.

10. 发明申请

WO2013037609A2 VERFAHREN ZUR ANPASSUNG EINES HYDROLYTISCHEN ENZYMS AN EINE DAS HYDROLYTISCHE ENZYM STABILISIERENDE KOMPONENTE 审中-公开
标题翻译： METHOD TO ALIGN水解酶AN水解酶稳定化成分
公开(公告)号：WO2013037609A2
公开(公告)日：2013-03-21
申请号：PCT/EP2012/066237
申请日：2012-08-21
申请人： HENKEL AG & CO. KGAA , SIEGERT, Petra , EVERS, Stefan , MERKEL, Marion , MUßMANN, Nina , HELLMUTH, Hendrik , O'CONNELL, Timothy , MAURER, Karl-Heinz , SCHWANEBERG, Ulrich , JAKOB, Felix , MARTINEZ, Ronny , LAUFS, Brian , AYDEMIR, Ayhan , WEBER, Thomas
发明人： SIEGERT, Petra , EVERS, Stefan , MERKEL, Marion , MUßMANN, Nina , HELLMUTH, Hendrik , O'CONNELL, Timothy , MAURER, Karl-Heinz , SCHWANEBERG, Ulrich , JAKOB, Felix , MARTINEZ, Ronny , LAUFS, Brian , AYDEMIR, Ayhan , WEBER, Thomas
IPC分类号： C11D3/386 , C12N9/54
CPC分类号： C12Q1/37 , C11D3/38663 , C11D3/38681 , C12N9/54 , C12N9/96
摘要： Die Stabilisierung eines hydrolytischen Enzyms in einer flüssigen Zubereitung soll durch eine das hydrolytische Enzym stabilisierende Komponente verbessert werden. Dies gelingt durch ein Verfahren zur Anpassung eines hydrolytischen Enzyms an eine das hydrolytische Enzym stabilisierende Komponente, das die folgenden Verfahrensschritte umfasst: a) Bereitstellen eines hydrolytischen Enzyms (Ausgangsenzym) und einer das hydrolytische Enzym stabilisierenden Komponente, die einen reversiblen Inhibitor des hydrolytischen Enzyms umfasst; b) Verändern der Aminosäuresequenz des hydrolytischen Enzyms an mindestens einer Position durch Substitution, Deletion oder Insertion; c) Bestimmen der relativen Aktivität des hydrolytischen Enzyms aus Schritt b) und der relativen Aktivität des Ausgangsenzyms in einer flüssigen Zubereitung; d) Auswählen desjenigen hydrolytischen Enzyms, das eine verminderte relative Aktivität aufweist im Vergleich mit der relativen Aktivität des Ausgangsenzyms.
摘要翻译：以液体制剂水解酶的稳定化是通过水解酶稳定组分得到改善。这是通过一种用于适配水解酶来水解酶稳定化组分，其包括以下步骤实现：a）提供水解酶（亲本酶）和包含水解酶的可逆抑制剂的水解酶稳定组分; b）改变在通过取代，缺失或插入至少一个位置上的水解酶的氨基酸序列; c）确定步骤b的水解酶）和以液体制剂的初始酶的相对活性的相对活性; d）选择具有相比于亲本酶的相对活性降低的相对活性的一种水解酶。

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