会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 3. 发明申请
    • METHODS AND COMPOSITIONS FOR RNA DETECTION AND QUANTITATION
    • RNA检测和定量的方法和组合物
    • WO2004068111A2
    • 2004-08-12
    • PCT/US2004/002316
    • 2004-01-29
    • QUANTA BIOSCIENCES, INC.SCHUSTER, David, M.RASHTCHIAN, Ayoub
    • SCHUSTER, David, M.RASHTCHIAN, Ayoub
    • G01N
    • C12Q1/686C12Q1/6806C12Q1/6846C12Q2600/158C12Q2547/101C12Q2521/107C12Q2521/101C12Q2527/137
    • The present invention generally relates to methods of making cDNA molecules, amplification of RNA by PCR and cDNA libraries. The invention also relates to kits for carrying out the methods of the invention. Methods for improved and more efficient conversion of RNA into cDNA are provided, which in turn can be used in a variety of procedures in molecular analysis of gene expression. The present invention is also directed to compositions comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.
    • 本发明一般涉及制备cDNA分子的方法,通过PCR和cDNA文库扩增RNA。 本发明还涉及用于实施本发明方法的试剂盒。 提供了用于改进和更有效地将RNA转化成cDNA的方法,其又可用于基因表达的分子分析中的各种程序。 本发明还涉及包含适合直接用于将RNA转化成cDNA和RT PCR的试剂混​​合物的组合物,所述试剂包括逆转录酶,缓冲液,辅因子和其他组分,所述试剂适合立即用于将RNA转化成cDNA和RT PCR而无需稀释或添加其他组分。 这些组合物可单独或以试剂盒的形式用于cDNA合成或核酸扩增(例如通过聚合酶链式反应)或用于在各种研究,医学,诊断,法医学和农业应用中利用逆转录酶的任何程序