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1. 发明申请

WO2007128441A3 A NOVEL GROUP OF ESTERASES FOR THE PRODUCTION OF FINE AND SPECIALITY CHEMICALS 审中-公开
标题翻译：一个生产精细和特种化学品的新一批产品
公开(公告)号：WO2007128441A3
公开(公告)日：2008-02-28
申请号：PCT/EP2007003772
申请日：2007-04-27
申请人： B R A I N AG , ELEND CHRISTIAN , JAEGER KARL-ERICH , LEGGEWIE CHRISTIAN , SCHMEISSER CHRISTEL , STREIT WOLFGANG
发明人： ELEND CHRISTIAN , JAEGER KARL-ERICH , LEGGEWIE CHRISTIAN , SCHMEISSER CHRISTEL , STREIT WOLFGANG
IPC分类号： C12N9/18
CPC分类号： C12N9/18
摘要： The present invention relates to a polynucleotide encoding an enzyme having carboxyl esterase [E. C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6 and 8; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5 and 7; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxyl esterase activity which polynucleotide is at least 65% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6 and 8; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (T) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host, genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a polypeptide comprising the amino acid sequence encoded by a polynucleotide of the present invention or which is obtainable by the process of the present invention. Moreover, the present invention relates to a process for producing said polypeptide and for producing bacteria expressing said polypeptide. Finally, the present invention relates to a composition comprising the polynucleotide of the present invention, the vector of the present invention, the host of the present invention, the polypeptide of the present invention, the antibody of the present invention and/or one or more primers of the present invention.
摘要翻译：本发明涉及编码具有羧基酯酶[E. 3.1.1.1]活性，其中编码序列选自（a）编码SEQ ID NO：2,4,6和8中任一个所示氨基酸序列的多核苷酸; （b）具有或包含编码酶的核苷酸序列的多核苷酸，其中所述核酸序列如SEQ ID NO：1,3,5和7中任一个所示; （c）具有或包含编码由（a）或（b）的多核苷酸编码的酶的片段或衍生物的核苷酸序列的多核苷酸，其中在所述衍生物中，与氨基酸相比，一个或多个氨基酸残基被保守取代（a）的顺序; （d）编码具有羧基酯酶活性的酶的多核苷酸与编码如SEQ ID NO：2,4,6和8之一所示的酶的多核苷酸至少65％相同; （e）具有或包含核苷酸序列的多核苷酸，其互补链与（a）至（d）中任一项所定义的多核苷酸杂交; 和（T）具有或包含与（d）或（e）的多核苷酸的核苷酸序列退化的核苷酸序列的多核苷酸; 或（a）至（f）的这种多核苷酸或其片段的互补链用作特异性探针或引物。本发明还涉及用本发明的多核苷酸或本发明的载体进行遗传工程改造的宿主。本发明还涉及包含由本发明的多核苷酸编码或可通过本发明的方法获得的氨基酸序列的多肽。此外，本发明涉及生产所述多肽和产生表达所述多肽的细菌的方法。最后，本发明涉及包含本发明的多核苷酸，本发明的载体，本发明的宿主，本发明的多肽，本发明的抗体和/或一种或多种的组合物本发明的引物。

2. 发明申请

WO2007128441A2 A NOVEL GROUP OF ESTERASES FOR THE PRODUCTION OF FINE AND SPECIALITY CHEMICALS 审中-公开
标题翻译：一个生产精细和特种化学品的新一批产品
公开(公告)号：WO2007128441A2
公开(公告)日：2007-11-15
申请号：PCT/EP2007/003772
申请日：2007-04-27
申请人： B.R.A.I.N. AG , ELEND, Christian , JAEGER, Karl-Erich , LEGGEWIE, Christian , SCHMEISSER, Christel , STREIT, Wolfgang
发明人： ELEND, Christian , JAEGER, Karl-Erich , LEGGEWIE, Christian , SCHMEISSER, Christel , STREIT, Wolfgang
IPC分类号： C12N9/18
CPC分类号： C12N9/18
摘要： The present invention relates to a polynucleotide encoding an enzyme having carboxyl esterase [E. C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6 and 8; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5 and 7; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxyl esterase activity which polynucleotide is at least 65% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6 and 8; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (T) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host, genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a polypeptide comprising the amino acid sequence encoded by a polynucleotide of the present invention or which is obtainable by the process of the present invention. Moreover, the present invention relates to a process for producing said polypeptide and for producing bacteria expressing said polypeptide. Finally, the present invention relates to a composition comprising the polynucleotide of the present invention, the vector of the present invention, the host of the present invention, the polypeptide of the present invention, the antibody of the present invention and/or one or more primers of the present invention.
摘要翻译：本发明涉及编码具有羧基酯酶[E. 3.1.1.1]活性，其中编码序列选自（a）编码SEQ ID NO：2,4,6和8中任一个所示氨基酸序列的多核苷酸; （b）具有或包含编码酶的核苷酸序列的多核苷酸，其中所述核酸序列如SEQ ID NO：1,3,5和7中任一个所示; （c）具有或包含编码由（a）或（b）的多核苷酸编码的酶的片段或衍生物的核苷酸序列的多核苷酸，其中在所述衍生物中，与氨基酸相比，一个或多个氨基酸残基被保守取代（a）的顺序; （d）编码具有羧基酯酶活性的酶的多核苷酸与编码如SEQ ID NO：2,4,6和8之一所示的酶的多核苷酸至少65％相同; （e）具有或包含核苷酸序列的多核苷酸，其互补链与（a）至（d）中任一项所定义的多核苷酸杂交; 和（T）具有或包含与（d）或（e）的多核苷酸的核苷酸序列退化的核苷酸序列的多核苷酸; 或（a）至（f）的这种多核苷酸或其片段的互补链用作特异性探针或引物。本发明还涉及用本发明的多核苷酸或本发明的载体进行遗传工程改造的宿主。本发明还涉及包含由本发明的多核苷酸编码或可通过本发明的方法获得的氨基酸序列的多肽。此外，本发明涉及生产所述多肽和产生表达所述多肽的细菌的方法。最后，本发明涉及包含本发明的多核苷酸，本发明的载体，本发明的宿主，本发明的多肽，本发明的抗体和/或一种或多种的组合物本发明的引物。

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