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1. 发明申请

WO2008009287A1 A MUTANT HISTIDINE KINASE THAT CONFERS SPONTANEOUS NODULATION IN PLANTS 审中-公开
标题翻译：在植物中配备自发性排斥的突变型发炎
公开(公告)号：WO2008009287A1
公开(公告)日：2008-01-24
申请号：PCT/DK2006/050031
申请日：2006-07-21
申请人： PLANT BIOSCIENCES LTD. , TIRICHINE, Leila , STOUGAARD, Jens , SANDAL, Niels, Nørgaard , MADSEN, Lene, H , RADUTOIU, Elena, Simona
发明人： TIRICHINE, Leila , STOUGAARD, Jens , SANDAL, Niels, Nørgaard , MADSEN, Lene, H , RADUTOIU, Elena, Simona
IPC分类号： C12N15/29 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8261 , C12N9/1205 , C12N15/8295 , Y02A40/146
摘要： Formation of nitrogen fixing root nodules in legumes is induced by perception of lipochitin-oligosaccharide signal molecules secreted by compatible Rhizobium bacteria, which triggers a common symbiotic pathway. The present invention provides a spontaneous nodule formation ( snf2 ) mutant, in which the formation of symbiotic nodules is spontaneous, leading to nodule development in the absence as well as in the presence of Rhizobium bacteria and/or exogenous rhizobial signals. The invention further provides an isolated DNA sequence encoding a mutant cytokinin-independent histidine kinase whose activity results in this `gain of function' dominant phenotype of spontaneous nodule formation. Furthermore the snf2 gene is shown to confer a phenotype, characterised by regulated organogenesis of spontaneous nodules, to plants having a nodulation deficient genetic background. A gene of the invention, that confers this spontaneous nodulation phenotype, has 15 utility for the transfer and establishment of nitrogen fixing capability in non- nodulating plants, and thereby reducing the nitrogen fertiliser dependence of non-nodulating crop plants.
摘要翻译：通过感受由兼容的根瘤细菌分泌的脂壳素 - 寡糖信号分子，引起豆类中氮固定根结节的形成，这触发了共同的共生途径。本发明提供自发结节形成（snf2）突变体，其中共生结节的形成是自发的，导致在不存在以及存在根瘤细菌和/或外源根瘤菌信号的情况下的结节发育。本发明还提供了编码突变型细胞分裂素非依赖性组氨酸激酶的分离的DNA序列，其活性导致自发结节形成的“增益功能”主要表型。此外，snf2基因显示赋予特征在于自发结节的器官发生特征的表型，具有结节缺乏遗传背景的植物。赋予这种自发结瘤表型的本发明基因在非结瘤植物中转移和建立固氮能力具有十五种效用，从而降低非结核作物的氮肥依赖性。

2. 发明申请

WO2007006318A3 SPONTANEOUS NODULATION IN PLANTS 审中-公开
公开(公告)号：WO2007006318A3
公开(公告)日：2007-01-18
申请号：PCT/DK2006/050029
申请日：2006-07-13
申请人： AARHUS UNIVERSITET , TIRICHINE, Leila , JENSEN, Jens, Stougaard , SANDAL, Niels , MADSEN, Lene, Heegard
发明人： TIRICHINE, Leila , JENSEN, Jens, Stougaard , SANDAL, Niels , MADSEN, Lene, Heegard
IPC分类号： C12N15/82 , A01H5/00
摘要： Formation of nitrogen fixing root nodules in legumes is induced by perception of lipochitin-oligosaccharide signal molecules secreted by compatible Rhizobium bacteria, which triggers a common symbiotic pathway. The present invention provides a spontaneous nodule formation (snf1-5g) mutant, in which the formation of symbiotic nodules is deregulated, leading to nodule development in the absence as well as in the presence of Rhizobium bacteria and/or exogenous rhizobial signals. The invention further provides an isolated DNA sequence encoding a mutant chimeric Ca 2+ /calmodulin dependent protein kinase whose activity results in this `gain of function' phenotype of spontaneous nodule formation. Furthermore the snf1-5g gene is shown to confer a spontaneous nodule formation phenotype to plants having a nodulation deficient genetic background. A gene of the invention, that confers a spontaneous nodulation phenotype, has utility for the transfer and establishment of nitrogen fixing capability in non-nodulating plants, and thereby reducing the nitrogen fertiliser dependence of non-nodulating crop plants.

3. 发明申请

WO2007006318A2 SPONTANEOUS NODULATION IN PLANTS 审中-公开
标题翻译：植物中的自发性播种
公开(公告)号：WO2007006318A2
公开(公告)日：2007-01-18
申请号：PCT/DK2006050029
申请日：2006-07-13
申请人： UNIV AARHUS , TIRICHINE LEILA , JENSEN JENS STOUGAARD , SANDAL NIELS , MADSEN LENE HEEGARD
发明人： TIRICHINE LEILA , JENSEN JENS STOUGAARD , SANDAL NIELS , MADSEN LENE HEEGARD
CPC分类号： C12N15/8271 , C12N9/1205 , C12N15/8261 , Y02A40/146
摘要： Formation of nitrogen fixing root nodules in legumes is induced by perception of lipochitin-oligosaccharide signal molecules secreted by compatible Rhizobium bacteria, which triggers a common symbiotic pathway. The present invention provides a spontaneous nodule formation (snf1-5g) mutant, in which the formation of symbiotic nodules is deregulated, leading to nodule development in the absence as well as in the presence of Rhizobium bacteria and/or exogenous rhizobial signals. The invention further provides an isolated DNA sequence encoding a mutant chimeric Ca 2+ /calmodulin dependent protein kinase whose activity results in this `gain of function' phenotype of spontaneous nodule formation. Furthermore the snf1-5g gene is shown to confer a spontaneous nodule formation phenotype to plants having a nodulation deficient genetic background. A gene of the invention, that confers a spontaneous nodulation phenotype, has utility for the transfer and establishment of nitrogen fixing capability in non-nodulating plants, and thereby reducing the nitrogen fertiliser dependence of non-nodulating crop plants. 20
摘要翻译：通过感受由兼容的根瘤细菌分泌的脂壳素 - 寡糖信号分子，引起豆类中氮固定根结节的形成，这触发了共同的共生途径。本发明提供了自发结节形成（snf1-5g）突变体，其中共生结节的形成被解除调节，导致在不存在以及存在根瘤细菌和/或外源根瘤菌信号的情况下的结节发育。本发明进一步提供编码突变嵌合型Ca 2+ /钙调蛋白依赖性蛋白激酶的分离的DNA序列，其活性导致自发结节形成的这种“功能增强”表型。此外，snf1-5g基因被证明赋予具有结节缺乏遗传背景的植物的自发结节形成表型。赋予自发结瘤表型的本发明的基因对于非结瘤植物的转移和建立氮固定能力具有效用，从而降低非结核作物的氮肥依赖性。 20

4. 发明申请

WO2005003338A1 NOD-FACTOR PERCEPTION 审中-公开
标题翻译： NOD因子观念
公开(公告)号：WO2005003338A1
公开(公告)日：2005-01-13
申请号：PCT/DK2004/000478
申请日：2004-07-02
申请人： AARHUS UNIVERSITET , JENSEN, Jens, Stougaard , MADSEN, Lene, Heegaard , RADUTOIU, Elena, Simona , MADSEN, Esben, Bjørn , SANDAL, Niels, Nørgaard
发明人： JENSEN, Jens, Stougaard , MADSEN, Lene, Heegaard , RADUTOIU, Elena, Simona , MADSEN, Esben, Bjørn , SANDAL, Niels, Nørgaard
IPC分类号： C12N9/12
CPC分类号： C12N9/1205 , C12N15/8261 , Y02A40/146
摘要： The present invention provides a Nod-factor binding element, comprising one or more NFR polypeptides encoded by NFR genes, that are useful for providing non-nodulating plants with Nod-factor binding properties and triggering the endosymbiotic signalling pathway leading to nodulation. Furthermore the invention is useful for breeding for improved nodulation in nodulating legumes.
摘要翻译：本发明提供了一种Nod因子结合元件，其包含由NFR基因编码的一种或多种NFR多肽，其可用于向非结瘤植物提供Nod-因子结合特性并引发导致结瘤的内共生信号通路。此外，本发明可用于育种以改善结瘤豆类中的结瘤。

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