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1. 发明申请

WO1997010330A1 COMBINED LIGAND AND RECEPTOR DISPLAY 审中-公开
标题翻译：组合配对和受体显示
公开(公告)号：WO1997010330A1
公开(公告)日：1997-03-20
申请号：PCT/GB1996002271
申请日：1996-09-13
申请人： BIOINVENT INTERNATIONAL AB , KIDDLE, Simon, John , BORREBAECK, Carl , MALMBORG, Anki , SÖDERLIND, Eskil
发明人： BIOINVENT INTERNATIONAL AB , KIDDLE, Simon, John
IPC分类号： C12N15/10
CPC分类号： C40B40/02 , C07K14/005 , C07K14/245 , C07K2319/00 , C12N15/1037 , C12N15/1055 , C12N15/70 , C12N2710/16122 , C12N2740/16222
摘要： A method for selecting nucleic acid sequences encoding ligand and receptor molecules capable of specific binding to each other is disclosed in which nucleic acid encoding ligand or receptor molecules is expressed in a host microorganism in combination with a surface molecule, such as E. coli pili, so that the ligand or receptor are displayed on the surface of the host microorganism. A replicable genetic unit, such as a filamentous bacteriophage, is used to display candidate binding partners to the ligand or receptor, with the binding of the ligand or receptor to the candidate binding partner mediating the transfer of nucleic acid from the replicable genetic unit to the microorganism. The method can be highly selective as the host microorganism is modified so that it does not display the surface molecule other than as a fusion with the ligand or receptor molecule. The method is rapid and simple and opens up new applications based on the detection of ligand and receptors where both are unknown.
摘要翻译：公开了选择能够相互特异性结合的配体和受体分子的核酸序列的方法，其中编码配体或受体分子的核酸在宿主微生物中与表面分子如大肠杆菌大肠杆菌，使得配体或受体显示在宿主微生物的表面上。可复制的遗传单位，例如丝状噬菌体，用于向配体或受体显示候选结合配偶体，其中配体或受体与候选结合配偶体结合，该候选结合配偶体介导核酸从可复制遗传单位转移至微生物。该方法可以是高度选择性的，因为宿主微生物被修饰，使得它不显示表面分子而不是与配体或受体分子的融合。该方法快速简单，并且基于配体和受体的检测开启了新的应用，其中两者都是未知的。

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