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    • 2. 发明申请
    • QUANTIFYING NUCLEIC ACID IN SAMPLES
    • 在样品中定量核酸
    • WO2012076336A1
    • 2012-06-14
    • PCT/EP2011/070798
    • 2011-11-23
    • QIAGEN LAKE CONSTANCE GMBHQUINTEL, HaraldFEUERBACHER, Elmar, ChristianGRULER, RomanHUCKINS, Stuart, RonaldZAHR, DominikREBHOLZ, MarkusSCHILD, Robert
    • QUINTEL, HaraldFEUERBACHER, Elmar, ChristianGRULER, RomanHUCKINS, Stuart, RonaldZAHR, DominikREBHOLZ, MarkusSCHILD, Robert
    • G01J3/10G01J3/36G01J3/427G01N21/33
    • G01J3/42G01J3/10G01J3/36G01J3/427G01N21/33
    • A photometric device (18) for quantifying a nucleic acid in a sample (518) in a tube (58), comprises a light emission unit (28), a sample obtaining unit (38) for holding the tube (58) with the nucleic acid sample (518) and a detection unit (48) wherein the sample obtaining unit (38) is arranged in between the light emission unit (28) and the detection unit (48) In particular, the light emission unit (28) and the detection unit (48) are arranged to provide light through the sample obtaining unit (38) such that light of a first wavelength of about 230 nanometers and light of a second wavelength of about 260 nanomaters are simultaneously detectable within the detection unit (48). The photometric device (18) allows for efficiently analysing the nucleic acid sample by only considering invisible light. In particular, content of nucleic acid such as particularly a RNA or a DNA and a nucleic acid/salt ratio can simultaneously be determined such that efficiency of quantification of the nucleic acid sample (518) can be increased. Furthermore, since in addition thereto the sample obtaining unit (38) is arranged to hold the tube (58) or cuvette and the light directly passes the sample (518) being arranged in the tube (58) or cuvette, loss of sample, contamination of sample, need of additional sample treatment equipment such as pipettes or additional tubes as well as frequent cleaning of optics of the photometric device can be prevented or reduced.
    • 用于量化管(58)中的样品(518)中的核酸的光度测量装置(18)包括发光单元(28),用于将核(58)保持在核上的样品获得单元(38) 酸样品(518)和检测单元(48),其中样品获得单元(38)布置在发光单元(28)和检测单元(48)之间。具体地,发光单元(28)和 检测单元(48)被布置成通过样品获得单元(38)提供光,使得在检测单元(48)内同时可检测到约230纳米的第一波长的光和约260纳米的第二波长的光。 光度测量装置(18)允许通过仅考虑不可见光来有效地分析核酸样品。 特别地,可以同时确定诸如特别是RNA或DNA的核酸的含量和核酸/盐比,从而可以提高核酸样品(518)的定量效率。 此外,由于样品获取单元(38)除此之外还被配置成保持管(58)或试管,并且光直接通过布置在管(58)或试管中的样品(518),样品损失,污染 的样品,可以防止或减少额外的样品处理设备如移液管或附加管的需要,并且可以频繁地清洁光度测量装置的光学元件。