专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2005103234A1 A NEW METHOD OF EXPANDING CORD BLOOD CELLS 审中-公开
标题翻译：一种扩大牙髓细胞的新方法
公开(公告)号：WO2005103234A1
公开(公告)日：2005-11-03
申请号：PCT/CA2005/000628
申请日：2005-04-25
申请人： HÉMA-QUÉBEC , PROULX, Chantal , DUPUIS, Nicolas , LEMIEUX, Réal
发明人： PROULX, Chantal , DUPUIS, Nicolas , LEMIEUX, Réal
IPC分类号： C12N5/08
CPC分类号： C12N5/0647 , C12N5/0644 , C12N2500/02 , C12N2501/125 , C12N2501/145 , C12N2501/23 , C12N2501/26
摘要： Based on previous evidence suggesting positive effects of fever on in vivo hematopoiesis, the effect of hyperthermia on the expansion and differentiation of megakaryocytes (MKs) in ex vivo cultures of CB CD34-enriched cells has now been tested. Cells were cultured at 37°C or 39°C for 14 days in cytokine conditions optimized for MK development, and analyzed periodically by microscopy, flow cytometry and colony assays. Compared to 37°C, cultures maintained at 39°C produced much more total cells (5X), MK progenitors (9X) and total MKs (7X), and showed accelerated (3-4 days) and enhanced MK maturation with increased yields of proplatelets and platelets (11.7X). The increased number of CD34+ cells and myeloid progenitors in the 39°C cultures also suggested a general stimulatory effect of hyperthermia on the expansion of more primitive stem/progenitor cells and of cells of other lineages.
摘要翻译：基于先前证据表明发热对体内造血作用的积极影响，现在已经测试了高热对CB CD34富集细胞离体培养物中巨核细胞（MK）的扩增和分化的影响。细胞在37℃或39℃下在针对MK发育优化的细胞因子条件下培养14天，并通过显微镜，流式细胞术和菌落测定定期分析。与37℃相比，保持在39℃的培养物产生更多的总细胞（5X），MK祖细胞（9X）和总MK（7X），并显示加速（3-4天）并增强MK成熟，增加产量血小板和血小板（11.7倍）。在39℃培养物中CD34 +细胞和骨髓祖细胞数量的增加也表明热疗对更原始的干/祖细胞和其他谱系的细胞的扩增具有一般的刺激作用。

IPRDB

热门服务

关于我们

友情链接

联系方式