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    • 9. 发明申请
    • DETECTION OF CORROSION-INDUCING PROKARYOTES
    • 检测腐蚀诱导原核生物
    • WO2008066614A2
    • 2008-06-05
    • PCT/US2007/022147
    • 2007-10-17
    • MULTI-CHEM GROUP, LLCUNIVERSITY OF LOUISIANA AT LAFAYETTEMADRID, VanessaCHISTOSERDOV, AndreiCHAPMAN, MichaelPRICE, Brian
    • MADRID, VanessaCHISTOSERDOV, AndreiCHAPMAN, MichaelPRICE, Brian
    • C12Q1/68
    • C12Q1/689
    • A simple method for the collection of field samples for nucleic acid extraction and subsequential detection of microorganisms from various media such as aqueous samples, sediments and biofilms (sessile bacteria). This method is a quantitative polymerase chain reaction (PCR)-based technique for detection of SRP in various media (i.e., aqueous samples, sediments, biofilms). The principle of this technique is based on the fact that all prokaryotes (Bacteria and Archaea) able to carry out sulfate reduction possess a gene that encodes dissimilatory sulfite reductase (DSR), the key enzyme in the sulfate reduction pathway. Microbial induced corrosion is due to bacterial growth and thus, tests for the detection of bacterial growth in fluids involved in oil processing are routinely performed. Thus far, the widely accepted methods for detection of bacterial growth of microorganisms involved in corrosion are cultivation dependent and, therefore, these methods underestimate the number of microorganisms present in a sample and require long incubation times.
    • 用于收集来自各种介质如水样品,沉淀物和生物膜(无菌细菌)的微生物的核酸提取和后续检测的现场样品的简单方法。 该方法是一种基于定量聚合酶链反应(PCR)的技术,用于检测各种培养基(即水样,沉积物,生物膜)中的SRP。 该技术的原理是基于所有能够进行硫酸盐还原的原核生物(细菌和古细菌)都具有编码异硫酸盐还原酶(DSR)的基因,这是硫酸盐还原途径中的关键酶。 微生物诱导的腐蚀是由于细菌生长,因此常规地进行了涉及油加工的流体中细菌生长检测的检测。 到目前为止,被广泛接受的用于检测涉及腐蚀的微生物的细菌生长的方法是培养依赖性的,因此,这些方法低估了样品中存在的微生物数量,并且需要长的孵育时间。