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    • 2. 发明申请
    • NOVEL CELL WALL DECONSTRUCTION ENZYMES AND USES THEREOF
    • 新型细胞壁分解酶及其用途
    • WO2012093149A3
    • 2013-08-22
    • PCT/EP2012050130
    • 2012-01-05
    • DSM IP ASSETS BVVALORBEC SOC EN COMMANDITETSANG ADRIANPOWLOWSKI JUSTINBUTLER GREGORYSTORMS REGINALD
    • TSANG ADRIANPOWLOWSKI JUSTINBUTLER GREGORYSTORMS REGINALD
    • C12N9/24C12P7/06C12P19/00
    • C12N9/2402C12P7/06C12P19/00Y02E50/17
    • The present invention relates to a novel process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising a polypeptide having a) a polypeptide sequence as set forth in any one of SEQ ID Nos: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66 and 69; b) a polypeptide that is at least 80%, preferably at least 85%, more preferably at least 90%, even more preferably at least 95%, 96%, 97%, 98% or 99% homologous to the any one of SEQ ID Nos: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66 and 69; c) a polypeptide sequence encoded by nucleic acids sequence as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 and 68, or nucleic acids that are at least 80%, preferably at least 85%, more preferably at least 90%, even more preferably at least 95%, 96%, 97%, 98% or 99% homologous to any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 and 68; d) a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 and 68; or e) a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the reverse complement of a polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 and 68.
    • 本发明涉及一种将生物质或预处理的生物质降解成糖的新方法,其中使用的酶包括具有a)如SEQ ID No:3,6,9,12,15中任一项所示的多肽序列的多肽 ,18,21,24,27,30,33,36,39,42,45,48,51,54,57,60,63,66和69; b)与SEQ的任一个同源的至少80%,优选至少85%,更优选至少90%,甚至更优选至少95%,96%,97%,98%或99%的多肽 ID号:3,6,9,12,15,18,21,24,27,30,33,36,39,42,45,48,51,54,57,60,63,66和69; c)由SEQ ID NO:1,2,4,5,7,8,10,11,13,14,16,17,19,20,22中任一项所示的核酸序列编码的多肽序列 ,23,25,26,28,29,31,32,34,35,37,38,40,41,43,44,46,47,49,50,52,53,55,56,58,59 ,61,62,64,65,67和68,或至少80%,优选至少85%,更优选至少90%,甚至更优选至少95%,96%,97%的核酸, 与SEQ ID NO:1,2,4,5,7,8,10,11,13,14,16,17,19,20,22,23,25,26,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,2 28,29,31,32,34,35,37,38,40,41,43,44,46,47,49,50,52,53,55,56,58,59,61,62,64, 65,67和68; d)由在严格条件下与SEQ ID NO:2,4,5,7,8,10,11,13,14,16中任一项所示的多核苷酸杂交的核酸序列编码的多肽序列 ,17,19,20,22,23,25,26,28,29,31,32,34,35,37,38,40,41,43,44,46,47,49,50,52,53 ,55,56,58,59,61,62,64,65,67和68; 或e)由严格条件下杂交的核酸序列编码的多肽序列与如SEQ ID NO:1,2,4,5,7,8,10,11, 13,14,16,17,19,20,22,23,25,26,28,29,31,32,34,35,37,38,40,41,43,44,46,47,49, 50,52,53,55,56,58,59,61,62,64,65,67及68条。
    • 6. 发明申请
    • NOVEL CELL WALL DECONSTRUCTION ENZYMES OF THERMOMYCES LANUGINOSUS AND USES THEREOF
    • 新型细胞壁分解酶的酶解及其用途
    • WO2012130964A1
    • 2012-10-04
    • PCT/EP2012/055671
    • 2012-03-29
    • DSM IP ASSETS B.V.Valorbec sociéte en commanditeTSANG, AdrianPOWLOWSKI, JustinBUTLER, Gregory
    • TSANG, AdrianPOWLOWSKI, JustinBUTLER, Gregory
    • C12N9/42
    • D21C9/1036C07K14/37C08H6/00C08H8/00C10L1/023C11D3/38636C12N9/0061C12N9/1029C12N9/18C12N9/2402C12N9/2434C12N9/2437C12N9/2477C12P19/02C12P19/14D21C5/005Y02E50/16
    • The present invention relates to a process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising a polypeptide having a. a polypeptide sequence as set forth in any one of SEQ ID Nos: 3, 6, 9, 12, 15, 18, 21, 24, 27 and 30; b. a polypeptide that is at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%, 98% or 99% homologous to the any one of SEQ ID Nos: 3, 6, 9, 12, 15, 18, 21, 24, 27 and 30; c. a polypeptide sequence encoded by nucleic acids sequence as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28 and 29, or nucleic acids that are at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%, 98% or 99% homologous to any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28 and 29; a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28 and 29; or e. a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the reverse complement of a polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28 and 29.
    • 本发明涉及一种将生物质或预处理的生物质降解成糖的方法,其中使用的酶包括具有a的多肽。 如SEQ ID No:3,6,9,12,15,18,21,24,27和30中任一项所示的多肽序列; 湾 与下列任何一种的至少60%,优选至少70%,更优选至少80%,甚至更优选至少90%,95%,96%,97%,98%或99%同源的多肽 SEQ ID NO:3,6,9,12,15,18,21,24,27和30; C。 由SEQ ID NO:1,2,4,5,7,8,10,11,13,14,16,17,19,20,22,23中任一个所示的核酸序列编码的多肽序列 ,25,26,28和29,或至少60%,优选至少70%,更优选至少80%,甚至更优选至少90%,95%,96%,97%,98的核酸 与SEQ ID NO:1,2,4,5,7,8,10,11,13,14,16,17,19,20,22,23,25,26,28中的任一个同源的%或99% 和29; 由在严格条件下与如SEQ ID NO:2,4,5,7,8,10,11,13,14,16,17中任一项所示的多核苷酸杂交的核酸序列编码的多肽序列 ,19,20,22,23,25,26,28和29; 或e。 由在严格条件下与SEQ ID NO:2,4,5,7,8,10,11,13,14中任一项所示的多核苷酸的反向互补序列杂交的核酸序列编码的多肽序列 ,16,17,19,20,22,23,25,26,28及29条。
    • 7. 发明申请
    • NOVEL CELL WALL DECONSTRUCTION ENZYMES OF TALAROMYCES THERMOPHILUS AND USES THEREOF
    • TALAROMYCES热电偶的新型细胞壁分解酶及其用途
    • WO2012130950A1
    • 2012-10-04
    • PCT/EP2012/055654
    • 2012-03-29
    • DSM IP ASSETS B.V.VALORBEC SOCIÉTE EN COMMANDITETSANG, AdrianPOWLOWSKI, JustinBUTLER, Gregory
    • TSANG, AdrianPOWLOWSKI, JustinBUTLER, Gregory
    • C12P19/02C12P7/10C12N9/42
    • C12N9/2434C11D3/38636C11D3/38645C12P7/10C12P19/02D21C9/1036Y02E50/16
    • The present invention relates to a process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising a polypeptide having a. a polypeptide sequence as set forth in any one of SEQ ID Nos: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39 and 42; b. a polypeptide that is at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%, 98% or 99% homologous to the any one of SEQ ID Nos: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39 and 42; c. a polypeptide sequence encoded by nucleic acids sequence as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40 and 41, or nucleic acids that are at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%, 98% or 99% homologous to any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 1 1, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40 and 41; d. a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40 and 41; or e. a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the reverse complement of a polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40 and 41.
    • 本发明涉及一种将生物质或预处理的生物质降解成糖的方法,其中使用的酶包括具有a的多肽。 SEQ ID No:3,6,9,12,15,18,21,24,27,30,33,36,39和42中任一项所示的多肽序列; 湾 与下列任何一种的至少60%,优选至少70%,更优选至少80%,甚至更优选至少90%,95%,96%,97%,98%或99%同源的多肽 SEQ ID NO:3,6,9,12,15,18,21,24,27,30,33,36,39和42; C。 由SEQ ID NO:1,2,4,5,7,8,10,11,13,14,16,17,19,20,22,23中任一项所示的核酸序列编码的多肽序列 ,25,26,28,29,31,32,34,35,37,38,40和41,或至少60%,优选至少70%,更优选至少80%的核酸,甚至更多 优选至少90%,95%,96%,97%,98%或99%同源于SEQ ID NO:1,2,4,5,7,8,10,11,13,14, 16,17,19,20,22,23,25,26,28,29,31,32,34,35,37,38,40及41条; 天。 由在严格条件下与如SEQ ID NO:2,4,5,7,8,10,11,13,14,16,17中任一项所示的多核苷酸杂交的核酸序列编码的多肽序列 ,19,20,22,23,25,26,28,29,31,32,34,35,37,38,40及41条; 或e。 由在严格条件下与SEQ ID NO:2,4,5,7,8,10,11,13,14中任一项所示的多核苷酸的反向互补序列杂交的核酸序列编码的多肽序列 ,16,17,19,20,22,23,25,26,28,29,31,32,34,35,37,38,40及41条。
    • 9. 发明申请
    • NOVEL CELL WALL DECONSTRUCTION ENZYMES AND USES THEREOF
    • 新型细胞壁分解酶及其用途
    • WO2012093149A2
    • 2012-07-12
    • PCT/EP2012/050130
    • 2012-01-05
    • DSM IP ASSETS B.V.VALORBEC SOCIETE EN COMMANDITETSANG, AdrianPOWLOWSKI, JustinBUTLER, GregorySTORMS, Reginald
    • TSANG, AdrianPOWLOWSKI, JustinBUTLER, GregorySTORMS, Reginald
    • C12N1/00
    • C12N9/2402C12P7/06C12P19/00Y02E50/17
    • The present invention relates to a novel process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising a polypeptide having a) a polypeptide sequence as set forth in any one of SEQ ID Nos: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66 and 69; b) a polypeptide that is at least 80%, preferably at least 85%, more preferably at least 90%, even more preferably at least 95%, 96%, 97%, 98% or 99% homologous to the any one of SEQ ID Nos: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66 and 69; c) a polypeptide sequence encoded by nucleic acids sequence as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 and 68, or nucleic acids that are at least 80%, preferably at least 85%, more preferably at least 90%, even more preferably at least 95%, 96%, 97%, 98% or 99% homologous to any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 and 68; d) a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 and 68; or e) a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the reverse complement of a polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 and 68.
    • 本发明涉及一种将生物质或预处理的生物质降解成糖的新方法,其中使用的酶包括具有a)如SEQ ID No:3,6,9,12,15中任一项所示的多肽序列的多肽 ,18,21,24,27,30,33,36,39,42,45,48,51,54,57,60,63,66和69; b)与SEQ的任一个同源的至少80%,优选至少85%,更优选至少90%,甚至更优选至少95%,96%,97%,98%或99%的多肽 ID号:3,6,9,12,15,18,21,24,27,30,33,36,39,42,45,48,51,54,57,60,63,66和69; c)由SEQ ID NO:1,2,4,5,7,8,10,11,13,14,16,17,19,20,22中任一项所示的核酸序列编码的多肽序列 ,23,25,26,28,29,31,32,34,35,37,38,40,41,43,44,46,47,49,50,52,53,55,56,58,59 ,61,62,64,65,67和68,或至少80%,优选至少85%,更优选至少90%,甚至更优选至少95%,96%,97%的核酸, 与SEQ ID NO:1,2,4,5,7,8,10,11,13,14,16,17,19,20,22,23,25,26,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,29,2 28,29,31,32,34,35,37,38,40,41,43,44,46,47,49,50,52,53,55,56,58,59,61,62,64, 65,67和68; d)由在严格条件下与SEQ ID NO:2,4,5,7,8,10,11,13,14,16中任一项所示的多核苷酸杂交的核酸序列编码的多肽序列 ,17,19,20,22,23,25,26,28,29,31,32,34,35,37,38,40,41,43,44,46,47,49,50,52,53 ,55,56,58,59,61,62,64,65,67和68; 或e)由严格条件下杂交的核酸序列编码的多肽序列与如SEQ ID NO:1,2,4,5,7,8,10,11, 13,14,16,17,19,20,22,23,25,26,28,29,31,32,34,35,37,38,40,41,43,44,46,47,49, 50,52,53,55,56,58,59,61,62,64,65,67及68条。