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1. 发明申请

WO2012049066A2 REAL-TIME AMPLIFICATION AND MICRO-ARRAY BASED DETECTION OF NUCLEIC ACID TARGETS IN A FLOW CHIP ASSAY 审中-公开
标题翻译：流式芯片测定中的核酸目标的实时放大和基于微阵列的检测
公开(公告)号：WO2012049066A2
公开(公告)日：2012-04-19
申请号：PCT/EP2011/067455
申请日：2011-10-06
申请人： EPPENDORF AG , ALEXANDRE, Isabelle , DEROECK, Sven , REMACLE, Jose , PLUESTER, Wilhelm
发明人： ALEXANDRE, Isabelle , DEROECK, Sven , REMACLE, Jose , PLUESTER, Wilhelm
IPC分类号： B01L7/00 , B01L3/00 , G01N21/64 , C12Q1/68
CPC分类号： C12Q1/6837 , B01L3/502784 , B01L7/525 , B01L2300/0636 , B01L2300/0816 , B01L2300/088 , B01L2300/0883 , C12Q1/6851 , C12Q1/6876 , C12Q2561/113 , C12Q2565/629
摘要： The present method is related to a method for identification and/or quantification of at least one polynucleotide target compound present in a biological sample among possible other ones by its amplification in a cycling flow chip solution passing through different temperatures required for the amplification and its detection in real-time onto a micro-array of specific capture molecules.
摘要翻译：本方法涉及通过在通过扩增和检测所需的不同温度的循环流芯片溶液中的扩增来鉴定和/或定量存在于生物样品中的至少一种多核苷酸靶化合物在可能的其它样品中的方法实时地对特定捕获分子的微阵列。

2. 发明申请

WO0159098A9 METHOD FOR PURIFYING NUCLEIC ACIDS 审中-公开
标题翻译：方法提纯的NUCLEIC
公开(公告)号：WO0159098A9
公开(公告)日：2003-07-31
申请号：PCT/EP0101134
申请日：2001-02-02
申请人： EPPENDORF AG , KOLZAU THOMAS , KOEHN HEINZ GERHARD , PLUESTER WILHELM , ULBRICHT MATHIAS
发明人： KOLZAU THOMAS , KOEHN HEINZ GERHARD , PLUESTER WILHELM , ULBRICHT MATHIAS
IPC分类号： C12N15/10 , C07H1/08
CPC分类号： C12N15/1017
摘要： The invention relates to a method for purifying nucleic acids from a nucleic acid containing starting material by means of a porous membrane. According to the inventive method, the optionally pre-purified starting material is mixed with a binding buffer that contains a filler in a concentration sufficient to adjust the final concentration in the mixture of starting material and binding buffer above the value required for precipitating nucleic acids. The binding buffer containing the starting material is left to permeate the membrane, whereby the nucleic acids are selectively bound to the surface or in the pores of the membrane. The membrane is optionally washed, and the nucleic acids are optionally eluted from the membrane in an additional step.
摘要翻译：一种用于净化从nukleinsäurehaltigem起始材料的核酸的多孔膜，其中，被添加到任选预纯化原料与包含填充剂在被调节在起始原料和结合上述用于缓冲的混合物中的最终浓度的浓度的结合缓冲液中的方法核酸所需的值的析出，可以渗透的结合缓冲液，通过该膜，其中所述核酸被选择性地保留在表面上或在膜中，该膜的孔，如果需要的话含起始原料，洗涤并再次任选地结合的核酸在进一步的步骤从膜洗脱。

3. 发明申请

WO2012049066A3 REAL-TIME AMPLIFICATION AND MICRO-ARRAY BASED DETECTION OF NUCLEIC ACID TARGETS IN A FLOW CHIP ASSAY 审中-公开
标题翻译：实时扩增和基于微阵列的流动芯片测试中核酸靶标的检测
公开(公告)号：WO2012049066A3
公开(公告)日：2012-06-07
申请号：PCT/EP2011067455
申请日：2011-10-06
申请人： EPPENDORF AG , ALEXANDRE ISABELLE , DEROECK SVEN , REMACLE JOSE , PLUESTER WILHELM
发明人： ALEXANDRE ISABELLE , DEROECK SVEN , REMACLE JOSE , PLUESTER WILHELM
IPC分类号： B01L7/00 , B01L3/00 , C12Q1/68 , G01N21/64
CPC分类号： C12Q1/6837 , B01L3/502784 , B01L7/525 , B01L2300/0636 , B01L2300/0816 , B01L2300/088 , B01L2300/0883 , C12Q1/6851 , C12Q1/6876 , C12Q2561/113 , C12Q2565/629
摘要： The present method is related to a method for identification and/or quantification of at least one polynucleotide target compound present in a biological sample among possible other ones by its amplification in a cycling flow chip solution passing through different temperatures required for the amplification and its detection in real-time onto a micro-array of specific capture molecules.
摘要翻译：本方法涉及一种通过在循环流动芯片溶液中扩增而在生物样品中存在于生物样品中的至少一种多核苷酸靶标化合物进行鉴定和/或定量的方法，所述循环流动芯片溶液经过扩增和检测所需的不同温度实时到特定捕获分子的微阵列上。

4. 发明申请

WO9932080A3 DEVICE FOR TARGETED IMMOBILIZATION OF THE THROMBOGENIC INHIBITOR HIRUDINE ON POLYMER SURFACES 审中-公开
标题翻译：方法对聚合物表面THROMBOGENESEINHIBITORS水蛭素有针对性的固定化
公开(公告)号：WO9932080A3
公开(公告)日：1999-10-14
申请号：PCT/DE9803727
申请日：1998-12-18
申请人： HOECKER HARTWIG , LAHANN JOERG , PLUESTER WILHELM , KLEE DORIS
发明人： HOECKER HARTWIG , LAHANN JOERG , PLUESTER WILHELM , KLEE DORIS
IPC分类号： A61K38/58 , A61L33/12 , A61L33/00
CPC分类号： A61L33/128 , A61K38/58
摘要： Disclosed is a method for immobilizing the thrombogenic inhibitor hirudine on polymer surfaces of extracorporeal and/or intracorporeal medical objects by direct interaction or via appropriate spacer systems between functional groups of the hirudine and the corresponding functional groups of the polymers. The chemical and/or spatial environment of the hirudine functional groups is modified as regards their immobilization selectivity in a treatment step prior to immobilization. After immobilization, the chemical and/or spatial environment is no longer modified. The proposed procedure enables the functional groups needed for thrombin activity not to be bound during immobilization and are thus available for interacting with thrombin after immobilization. This causes an increased antithrombogenic and/or thrombocyte-repelling effect of the hirudine bound on the surfaces.
摘要翻译：在用于固定Thrombogeneseinhibitors水蛭素上体外的聚合物的表面和/或intracorporally到医用制品由直接或通过合适的Spacersysteme对应的聚合物的官能团的水蛭素的官能团之间的相互作用可以使用，提出了一种方法，该方法在固定化上游处理步骤中的一个，所述化学和/或水蛭素的官能团的空间环境中的Immobilisierungsselektivität方面发生变化，并且在固定之后，在化学和/或物理环境的变化将再次高。所提出的方法可以确保必要的官能团不会在凝血酶活性的固定化结合，并因此可用于提供固定之后与凝血酶的相互作用。这会导致接合面水蛭素的增加的抗血栓形成和/或血小板排斥效果。

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