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1. 发明申请

WO2006008653A9 LIPOLYTIC ENZYME USES THEREOF IN THE FOOD INDUSTRY 审中-公开
标题翻译：脂肪酶在食品工业中的应用
公开(公告)号：WO2006008653A9
公开(公告)日：2007-02-22
申请号：PCT/IB2005002602
申请日：2005-07-18
申请人： DANISCO , MIASNIKOV ANDREI , SOEE JOERN BORCH , MIKKELSEN JOERN DALGAARD , POVELAINEN MIRA , PITKANEN VIRVE
发明人： MIASNIKOV ANDREI , SOEE JOERN BORCH , MIKKELSEN JOERN DALGAARD , POVELAINEN MIRA , PITKANEN VIRVE
IPC分类号： C11B3/00 , A21D2/26 , A23C9/13 , A23L9/10 , A23L13/00 , A23L13/40 , A23L13/50 , A23L15/00 , A23L17/00 , A23L27/24 , A23L27/60 , A23L29/00 , C07F9/10 , C11C3/00 , C12N9/20 , C12N15/55 , C12P7/64
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： The invention also encompases the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to a acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ. ID. No.s 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID No. 3, 6, 9, 13, 15 or 17 or a nulceotide seqeunce which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Steptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID No. 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3; b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3 by the degeneration of the genetic code; and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID No. 3.
摘要翻译：本发明还包括在各种方法和用途中可使用从下列属之一获得的脂解酶：链霉菌属，棒状杆菌属和嗜热菌属，其中所述脂解酶能够水解糖脂或磷脂或从糖脂转移酰基或磷脂酰基受体。优选地，用于这些方法和用途的脂解酶包含如SEQ.ID.NO.1中任一所示的氨基酸序列。 ID。编号为4,5,7,8,12,14或16的氨基酸序列或与其具有至少70％同一性的氨基酸序列，或者包含如SEQ ID No.3,6,9,13,15或17所示的核苷酸序列或与其具有至少70％同一性的核苷酸序列。本发明还涉及能够水解至少半乳糖脂或能够将酰基从半乳糖脂转移到一个或多个酰基受体底物的脂肪分解酶，其中所述酶可以从链霉菌种获得并且包括含有氨基酸的脂解酶如SEQ ID No.4所示的氨基酸序列或与其具有至少60％同一性的氨基酸序列。该酶可以由选自以下的核酸编码：a）包含SEQ ID No.3所示的核苷酸序列的核酸; b）通过遗传密码的变性与核苷酸序列SEQ ID No.3相关的核酸; 和c）包含与SEQ ID No.3所示核苷酸序列具有至少70％同一性的核苷酸序列的核酸。

2. 发明申请

WO2006008653A3 LIPOLYTIC ENZYME USES THEREOF IN THE FOOD INDUSTRY 审中-公开
标题翻译：脂肪酶在食品工业中的应用
公开(公告)号：WO2006008653A3
公开(公告)日：2007-03-29
申请号：PCT/IB2005002602
申请日：2005-07-18
申请人： DANISCO , MIASNIKOV ANDREI , SOEE JOERN BORCH , MIKKELSEN JOERN DALGAARD , POVELAINEN MIRA , PITKANEN VIRVE
发明人： MIASNIKOV ANDREI , SOEE JOERN BORCH , MIKKELSEN JOERN DALGAARD , POVELAINEN MIRA , PITKANEN VIRVE
IPC分类号： C11B3/00 , A21D2/26 , A23C9/13 , A23L9/10 , A23L13/00 , A23L13/40 , A23L13/50 , A23L15/00 , A23L17/00 , A23L27/24 , A23L27/60 , A23L29/00 , C07F9/10 , C11C3/00 , C12N9/20 , C12N15/55 , C12P7/64
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： The invention also encompases the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to a acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ. ID. No.s 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID No. 3, 6, 9, 13, 15 or 17 or a nulceotide seqeunce which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Steptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID No. 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3; b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3 by the degeneration of the genetic code; and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID No. 3.
摘要翻译：本发明还包括使用可从各种方法获得的脂肪分解酶：链霉菌，棒状杆菌和热嗜热菌，其中所述脂肪分解酶能够水解糖脂或磷脂或从糖脂转移酰基或磷脂转化为酰基受体。优选地，用于这些方法和用途的脂肪分解酶包含SEQ ID NO：1中任一项所示的氨基酸序列。 ID。第4,5,7,8,12,14或16号氨基酸序列或与其具有至少70％同一性的氨基酸序列，或包含SEQ ID No.3,6,13,13,15或17所示的核苷酸序列或与其具有至少70％同一性的nucceotide seqonunce。本发明还涉及能够至少水解半乳糖脂或能够将酰基从半乳糖脂转移至一种或多种酰基受体底物的脂解酶，其中所述酶可从步态物种获得，并且包括脂肪分解酶，其包含氨基如SEQ ID No.4所示的酸序列或与其具有至少60％同一性的氨基酸序列。该酶可以由选自以下的核酸编码：a）包含SEQ ID No.3所示核苷酸序列的核酸; b）通过遗传密码的退化与SEQ ID No.3的核苷酸序列相关的核酸; 和c）包含与SEQ ID No.3所示核苷酸序列具有至少70％同一性的核苷酸序列的核酸。

3. 发明申请

WO2006008653A2 LIPOLYTIC ENZYME USES THEREOF IN THE FOOD INDUSTRY 审中-公开
标题翻译：蛋白
公开(公告)号：WO2006008653A2
公开(公告)日：2006-01-26
申请号：PCT/IB2005/002602
申请日：2005-07-18
申请人： DANISCO A/S , MIASNIKOV, Andrei , SØE, Jørn, Borch , MIKKELSEN, Jørn, Dalgaard , POVELAINEN, Mira , PITKANEN, Virve
发明人： MIASNIKOV, Andrei , SØE, Jørn, Borch , MIKKELSEN, Jørn, Dalgaard , POVELAINEN, Mira , PITKANEN, Virve
IPC分类号： C11B3/00 , C11C3/00 , C07F9/10 , C12N15/55 , C12N9/20 , C12P7/64 , A23C9/13 , A21D2/26
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： The invention also encompases the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to a acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ. ID. No.s 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID No. 3, 6, 9, 13, 15 or 17 or a nulceotide seqeunce which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Steptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID No. 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3; b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3 by the degeneration of the genetic code; and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID No. 3.
摘要翻译：本发明还包括使用可从各种方法获得的脂肪分解酶：链霉菌，棒状杆菌和热嗜热菌，其中所述脂肪分解酶能够水解糖脂或磷脂或从糖脂转移酰基或磷脂转化为酰基受体。优选地，用于这些方法和用途的脂肪分解酶包含SEQ ID NO：1中任一项所示的氨基酸序列。 ID。第4,5,7,8,12,14或16号氨基酸序列或与其具有至少70％同一性的氨基酸序列，或包含SEQ ID No.3,6,13,13,15或17所示的核苷酸序列或与其具有至少70％同一性的nucceotide seqonunce。本发明还涉及能够至少水解半乳糖脂或能够将酰基从半乳糖脂转移至一种或多种酰基受体底物的脂解酶，其中所述酶可从步态物种获得，并且包括脂肪分解酶，其包含氨基如SEQ ID No.4所示的酸序列或与其具有至少60％同一性的氨基酸序列。该酶可以由选自以下的核酸编码：a）包含SEQ ID No.3所示核苷酸序列的核酸; b）通过遗传密码的退化与SEQ ID No.3的核苷酸序列相关的核酸; 和c）包含与SEQ ID No.3所示核苷酸序列具有至少70％同一性的核苷酸序列的核酸。

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